Histopathology of the Endometrium

Dallenbach-Hellweg, Gisela

doi:10.1007/978-3-642-96249-3

Cited by 46 publications

(65 citation statements)

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“…In the 1970's, Dallenbach-Hellweg et al . [17] proposed that the leukocytes were differentiated from undifferentiated endometrial stromal cells. In 2005, using an allogeneic transplantation model, Matsuura-Sawada et al .…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of Leukocytes, Vascularization and Matrix Metalloproteinases in Human Menstrual Xenograft Model

Guo

He²,

Xu³

et al. 2011

PLoS ONE

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In our previous study, menstrual-like changes in mouse were provoked through the pharmacologic withdrawal of progesterone with mifepristone following induction of decidualization. However, mouse is not a natural menstruation animal, and the menstruation model using external stimuli may not truly reflect the occurrence and development of the human menstrual process. Therefore, we established a model of menstruation based on human endometrial xenotransplantation. In this model, human endometrial tissues were transplanted subcutaneously into SCID mice that were ovarectomized and supplemented with estrogen and progestogen by silastic implants with a scheme imitating the endocrinological milieu of human menstrual cycle. Morphology, hormone levels, and expression of vimentin and cytokeratin markers were evaluated to confirm the menstrual-like changes in this model. With 28 days of hormone treatment, transplanted human endometrium survived and underwent proliferation, differentiation and disintegration, similar to human endometrium in vivo. Human CD45+ cells showed a peak of increase 28 days post-transplantation. Three days after progesterone withdrawal, mouse CD45+ cells increased rapidly in number and were significantly greater than human CD45+ cell counts. Mouse CD31+ blood vascular-like structures were detected in both transplanted and host tissues. After progesterone withdrawal, the expression levels of matrix metalloproteinases (MMP) 1, 2, and 9 were increased. In summary, we successfully established a human endometrial xenotransplantation model in SCID mice, based on the results of menstrual-like changes in which MMP-1, 2 and 9 are involved. We showed that leukocytes are originated from in situ proliferation in human xenografts and involved in the occurrence of menstruation. This model will help to further understand the occurrence, growth, and differentiation of the endometrium and the underlying mechanisms of menstruation.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of Leukocytes, Vascularization and Matrix Metalloproteinases in Human Menstrual Xenograft Model

Guo

He²,

Xu³

et al. 2011

PLoS ONE

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“…These patients had given their informed consent and their clinical characteristics are shown in table I. The day of cycle was determined by the clinical data and was compared with the histological data [10,21]. All the endometrial specimens were examined by the same anatomo-pathologist.…”

Section: Cell Culturesmentioning

confidence: 99%

“…Biopsies were performed in all periods of the menstrual cycle (table I), but mainly after day 14. These diagnostic procedures were undertaken for myomas [10], menometrorragias with endometrial hyperplasy [7], polyps [5], endometriosis and infertility (4 and 4).…”

Section: Cell Culturesmentioning

confidence: 99%

Normal human endometrial cells in culture: Characterization and immortalization of epithelial and stromal cells by SV 40 large T antigen

Merviel

Degeorges

Salat-Baroux

et al. 1995

Biology of the Cell

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Thirty endometrial biopsies were cultured in order to separate stromal and epithelial cells. Using epidermal growth factor (EGF), cortisol, cholera toxin, insulin with 5% horse serum for epithelial cells or a medium with 20% fetal calf serum for stromal cells, we could specifically enrich endometrial culture in epithelial or stromal cells and culture them for 1 or 2 months. These cultures retained the phenotypic characteristics of epithelial (cytokeratins, mucin HMFG 1) and stromal (vimentin, smooth muscle actin, desmin) lineage by immunostaining analysis. Epithelial and stromal cultures from one individual were respectively immortalized by the SV 40 large T antigen. The immortalized cell lines kept the phenotype of the normal cells from which they derived.

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“…She is well 4% years after the operation. -Hellweg (1975) states: "The histological diagnosis of a mature well-differentiated adeno-carcinoma in women under 40 years of age must be made with great caution." She goes on to say: "Growths, which histologically would be classified after the menopause as unequivocal adenocarcinomas, in young women are able to regress and therefore should be reported as adenomatous hyperplasia."…”

Section: Case Imentioning

confidence: 99%

Endometrial Carcinoma in Young Women

Östör

Adam

Gutteridge

et al. 1982

Aust NZ J Obst Gynaeco

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Endometrial carcinoma in young women is uncommon. The majority of cases occur in women who have been taking oestrogen-containing oral contraceptives, in those with the Stein-Leventhal syndrome, or in those with gonadal dysgenesis treated by long-term oestrogen replacement therapy. We have observed 4 women under 40 years of age with adenocarcinoma of the uterus in whom chromosomal abnormalities associated with Turner's syndrome were confirmed only after the diagnosis of carcinoma had been made. None of these had received replacement oestrogen therapy. It is postulated that chromosomal abnormalities in young women with endometrial carcinoma are more common than previously thought. As the disease in this group appears to behave in a benign fashion, a conservative approach to therapy is advocated.

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Histopathology of the Endometrium

Cited by 46 publications

References 0 publications

Comprehensive Analysis of Leukocytes, Vascularization and Matrix Metalloproteinases in Human Menstrual Xenograft Model

Comprehensive Analysis of Leukocytes, Vascularization and Matrix Metalloproteinases in Human Menstrual Xenograft Model

Normal human endometrial cells in culture: Characterization and immortalization of epithelial and stromal cells by SV 40 large T antigen

Endometrial Carcinoma in Young Women

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