Histopathology of <i>Verticillium dahliae</i> within mature roots of Russet Burbank potatoes

Perry, John; Evert, Ray F.

doi:10.1139/b83-384

Cited by 30 publications

(28 citation statements)

References 19 publications

(21 reference statements)

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“…This is the first report, to our knowledge, to use GFP to study V. dahliae, in planta, from the perspective of a compatible and incompatible interaction. While confirming many of the findings from earlier histochemical and immunoenzymatic studies in potato and cotton (6,21,22,43,44), this study also offered novel insights into V. dahliae pathogenesis from the early stages of root colonization through the advance stages of disease, including seed infection.…”

Section: Discussionsupporting

confidence: 81%

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Colonization of Resistant and Susceptible Lettuce Cultivars by a Green Fluorescent Protein-Tagged Isolate of Verticillium dahliae

Vallad

Subbarao²

2008

Phytopathology®

143

109

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Interactions between lettuce and a green fluorescent protein (GFP)-expressing, race 1 isolate of Verticillium dahliae, were studied to determine infection and colonization of lettuce cultivars resistant and susceptible to Verticillium wilt. The roots of lettuce seedlings were inoculated with a conidial suspension of the GFP-expressing isolate. Colonization was studied with the aid of laser scanning confocal and epi-fluorescence microscopes. Few differences in the initial infection and colonization of lateral roots were observed between resistant and susceptible cultivars. Hyphal colonies formed on root tips and within the root elongation zones by 5 days, leading to the colonization of cortical tissues and penetration of vascular elements regardless of the lettuce cultivar by 2 weeks. By 8 to 10 weeks after inoculation, vascular discoloration developed within the taproot and crown regions of susceptible cultivars well in advance of V. dahliae colonization. Actual foliar wilt coincided with the colonization of the taproot and crown areas and the eruption of mycelia into surrounding cortical tissues. Advance colonization of stems, pedicels, and inflorescence, including developing capitula and mature achenes was observed. Seedborne infection was limited to the maternal tissues of the achene, including the pappus, pericarp, integument, and endosperm; but the embryo was never compromised. Resistant lettuce cultivars remained free of disease symptoms. Furthermore, V. dahliae colonization never progressed beyond infected lateral roots of resistant cultivars. Results indicated that resistance in lettuce may lie with the plant's ability to shed infected lateral roots or to inhibit the systemic progress of the fungus through vascular tissues into the taproot.

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Section: Discussionsupporting

confidence: 81%

“…While numerous microscopic studies have examined the interaction of various hosts with V. dahliae, most focused on anatomic aspects of colonization (7,18,21,22,43,44,62). Few studies followed the colonization process through the entire disease cycle, and even fewer examined the role of race-specific resistance.…”

mentioning

confidence: 99%

Colonization of Resistant and Susceptible Lettuce Cultivars by a Green Fluorescent Protein-Tagged Isolate of Verticillium dahliae

Vallad

Subbarao²

2008

Phytopathology®

143

109

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“…The formation of a dark gum was observed to prevent hyphae from entering the root cortex of cotton (57). Whereas in potato, lignified cell wall appositions and lignitubers surrounded the invading hyphae at attempted penetration sites (111,112). Differences in the colonization pattern among resistant and susceptible crop cultivars have also been observed.…”

Section: Wwwannualreviewsorg • Verticillium Species 45mentioning

confidence: 95%

Diversity, Pathogenicity, and Management of Verticillium Species

Klosterman

Atallah

Vallad

et al. 2009

Annu. Rev. Phytopathol.

631

564

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The genus Verticillium encompasses phytopathogenic species that cause vascular wilts of plants. In this review, we focus on Verticillium dahliae, placing emphasis on the controversy surrounding the elevation of a long-spored variant as a new species, recent advances in the analysis of compatible and incompatible interactions, highlighted by the use of strains expressing fluorescent proteins, and the genetic diversity among Verticillium spp. A synthesis of the approaches to explore genetic diversity, gene flow, and the potential for cryptic recombination is provided. Control of Verticillium wilt has relied on a panoply of chemical and nonchemical strategies, but is beset with environmental or site-specific efficacy problems. Host resistance remains the most logical choice, but is unavailable in most crops. The genetic basis of resistance to Verticillium wilt is unknown in most crops, as are the subcellular signaling mechanisms associated with Ve-mediated, race-specific resistance. Increased understanding in each of these areas promises to facilitate management of Verticillium wilts across a broad range of crops.

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“…with various plant species, including olive, although mainly focused on anatomical aspects of the colonization process (for instance, Garber and Houston 1966;Perry and Evert 1983;Gerik and Huisman 1988;Rodríguez-Jurado 1993;Zhou et al 2006). Recently, the combination of biotechnological tools (construction of fluorescentlytagged V. dahliae derivatives) and powerful microscopic methodologies (confocal laser scanning microscopy [CLSM]), which allow direct in vivo observations of tissue samples, made it possible to examine in a very detailed way the V. dahliae colonization process in herbaceous hosts.…”

Section: Verticillium Wilt Of Olive Disease Cyclementioning

confidence: 99%

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

2010

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Olive (Olea europaea L.) is one of the first domesticated and cultivated tree species and has historical, social and economical relevance. However, its future as a strategic commodity in Mediterranean agriculture is threatened by diverse biotic (traditional and new/emerging pests and diseases) and abiotic (erosion, climate change) menaces. These problems could also be of relevance for new geographical areas where olive cultivation is not traditional but is increasingly spreading (i.e., South America, Australia, etc). One of the major constraints for olive cultivation is Verticillium wilt, a vascular disease caused by the soil-borne fungus Verticillium dahliae Kleb. In this review we describe how Verticillium wilt of olive (VWO) has become a major problem for olive cultivation during the last two decades. Similar to other vascular diseases, VWO is difficult to manage and single control measure are mostly ineffective. Therefore, an integrated disease management strategy that fits modern sustainable agriculture criteria must be implemented. Multidisciplinary research efforts and advances to understand this pathosystem and to develop appropriate control measures are summarized. The main conclusion is that a holistic approach is the best strategy to effectively control VWO, integrating biological, chemical, physical, and cultural approaches.

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Histopathology of Verticillium dahliae within mature roots of Russet Burbank potatoes

Cited by 30 publications

References 19 publications

Colonization of Resistant and Susceptible Lettuce Cultivars by a Green Fluorescent Protein-Tagged Isolate of Verticillium dahliae

Colonization of Resistant and Susceptible Lettuce Cultivars by a Green Fluorescent Protein-Tagged Isolate of Verticillium dahliae

Diversity, Pathogenicity, and Management of Verticillium Species

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

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