Histopathology and Immunohistochemical Expression of N-Methyl-N-Nitrosourea (NMU) Induced Mammary Tumours in Sprague-Dawley Rats

Saminathan, Mani; Rai, Ruchi; Dhama, Kuldeep; Ranganath, G.J.; Murugesan, Vignesh; Kannan, Kasthuri; Pavulraj, S.; Gopalakris, Arumugam; Suresh, C. Tyagi

doi:10.3923/ajava.2014.621.640

Cited by 9 publications

(8 citation statements)

References 20 publications

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“… 70 Our observation can be explained on the basis of the susceptibility of the mammary glands to the carcinogen on the age of animals and that the high susceptibility of female rats can be observed when the carcinogen is administered during the postnatal period, between 40 to 60 days of early puberty with highly proliferating terminal end buds (TEBs) in the mammary gland. 69 In agreement to a great extent with the prior studies, 67 our results indicated that all the developed tumors were in the posterior pairs of the mammary glands of the diseased rats. The authors reported that 36/44 of the diagnosed tumors in NMU-treated rats were developed in the abdominal inguinal mammary gland.…”

Section: Discussionsupporting

confidence: 92%

“…In agreement with the previous observation, our results showed a significant loss in weight gain in the positive control (diseased rats) as well as LRT treatment, which might be endorsed this effect to the tumor burden. 67 Rats showed a significant gain in body weight after administration of QMNPs either alone or in combination with LRT treatment. In a study performed previously by Najafabadi et al, 17 they demonstrated the effect of superparamagnetic iron oxide nanoparticles (SPION) and quercetin superparamagnetic iron oxide nanoparticles (QT-SPION) on body weight gain in treated rats.…”

Section: Discussionmentioning

confidence: 95%

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Synergistic Effect of Quercetin Magnetite Nanoparticles and Targeted Radiotherapy in Treatment of Breast Cancer

Askar

El-Nashar

Al-Azzawi

et al. 2022

Breast Cancer�(Auckl)

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Quercetin is a potent cancer therapeutic agent present in fruits and vegetables. The pharmaceutical uses of quercetin are limited due to many problems associated with low solubility, bioavailability, permeability, and instability. In addition, the high doses of quercetin show toxic effects in clinical and experimental studies. Therefore, a new strategy is warranted to overcome these problems without the use of toxic doses. The iron oxide nanoparticles can be used as a drug delivery system. This study aimed to prepare quercetin-conjugated magnetite nanoparticles (QMNPs) using biological simple nanoprecipitation and mediated by fungus Aspergillus oryzae. Also, we initiated in vitro and in vivo studies to determine whether QMNPs might sensitize breast cancer to radiotherapy treatment. The structural, morphological, and magnetic properties of the prepared nanoparticles were studied. The results indicated that QMNPs were spherical in shape and 40 nm in diameter. The in vitro studies showed that the incubation of MCF-7, HePG-2, and A459 cancer cells with QMNPs for 24 h effectively inhibited the growth of cancer cell lines in a concentration-dependent manner with IC50 values of 11, 77.5, and104 nmol/mL, respectively. The combination of QMNPs with irradiation (IR) potently blocked MCF-7 cancer cell proliferation and showed significant changes in the morphology of these cells as observed by bright-field inverted light microscopy. Focusing on the long-term toxicity of QMNPs (20 ml/kg), the assessment of hematological, hepatic, and renal markers indicated no toxic effect. Besides, QMNPs inhibited tumor growth and potently enhanced the lateral radiotherapy treatment in N-methyl-N-nitrosourea (MNU)-induced breast cancer in female white albino rats. These anticancer and radiosensitizing activities were ascribed to cytotoxicity, cell cycle arrest, immunomodulation, and efficiency through induction of apoptosis. In a conclusion, these observations suggest that the QMNPs combined with LRT could act as a potential targeted therapy in breast cancer.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 95%

Synergistic Effect of Quercetin Magnetite Nanoparticles and Targeted Radiotherapy in Treatment of Breast Cancer

Askar

El-Nashar

Al-Azzawi

et al. 2022

Breast Cancer�(Auckl)

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“…Food and water were available ad libitum and animals were weighed weekly during the experiment. Tumors were generated following published procedures by the administration of the carcinogens MNU [15,16] or DMBA as follows [17,18]. MNU was injected intraperitoneally at a dose of 50 mg/kg and a volume of 5 mL/kg body weight (n = 12).…”

Section: Research Animals and Tumor Inductionmentioning

confidence: 99%

Impact of Attenuation Correction on Quantification Accuracy in Preclinical Whole-Body PET Images

et al. 2020

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Background: Whole-body PET images can be obtained by using the "step-and-shoot" (SaS) method (using multiple bed positions) or continuous bed motion (CBM). As transmission scans are not always feasible, an alternative method where attenuation data can be generated via emission-based attenuation correction (AC) maps is of interest. The aim of this preclinical study was to investigate the influence of the acquisition method and AC on the quantitation accuracy of [ 18 F]FDG-PET. Methods: [ 18 F]FDG-PET phantom images were acquired using either SaS or CBM. Transmission scans were recorded for the SaS method using a 57 Co-point source. Emission-based attenuation sinograms were obtained from the images after segmentation and inverse Fourier rebinning. PET images were reconstructed without AC, transmission based (TX-AC) and emission-based (EM-AC) attenuation correction. Moreover, [ 18 F]FDG-PET scans of rats bearing mammary carcinomas acquired using either SaS or CBM were analyzed retrospectively and quantification in tissues was compared. Results: Phantom recovery coefficients (R C) varied greatly, ranging from 0.49 ± 0.01 to 1.15 ± 0.07, dependent on acquisition method, reconstruction algorithm and AC method. In CBM acquired images, EM-AC improved quantification accuracy when compared to no-AC images in the phantom studies (R C 0.79 ± 0.02 vs. 0.49 ± 0.01, respectively) and in tumors of rats (DMBA model: 1.16±0.42 SUV vs. 0.86±0.28 SUV, respectively). Conclusion: The method of AC has a strong influence on the quantification of [ 18 F]FDG. Our data indicates that EM-AC improves quantification in images obtained by CBM and SaS. However, the obtained values were still underestimated when compared to TX-AC corrected images.

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“…Female rats were injected intraperitoneally with (MNU) at a dose rate of (50 mg/kg body weight) five times periodically at 50, 70, 80, 90 and 110 days of age (Saminathan et al, 2014).…”

Section: Induction Of Mammary Tumormentioning

confidence: 99%

Effect of natural nanoparticles products on hepato-renal functions in breast carcinoma female rats

Zaid

Ahmed

Badwi

et al. 2017

Benha Veterinary Medical Journal

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The aim of this study was to evaluate the effect of natural nano-composite products in combination with doxorubicin chemotherapy treatment on serum biochemical profile in mammary cancer induced in fifty female rats divided as following; control normal n= 10, mammary carcinogenesis Induced group with MNU (50 mg/ kg. b. wt. / I.P dose) n=40 which divided into three subgroups (10 rats in each group): mammary carcinoma non-treated gp, doxorubicin treated group (33 mg /kg. b .wt. / I.P. dose), basic nano-curcumin (35 mg/ kg. b. wt. /oral dose) + doxorubicin treated group and basic nano-brown algae (70 mg /kg. b .wt. / oral dose) + doxorubicin treated group. Blood urea nitrogen (BUN) and serum creatinine level, AST and ALT activities were determined during the course of chemotherapy treatment among different groups. The level of BUN, creatinine and AST activity were more than normal reference range in doxorubicin chemotherapy treatment, basic nano-curcumin and basic nano-brown algae; while the value of serum ALT was reported to be within the normal reference range in case of treatment by using basic nano-curcumin with doxorubicin chemotherapy. In conclusion, the obtained results suggest that, treatment with chemotherapy and basic nano-curcumin in combination improve serum biochemical profile in breast carcinoma induced in rats.

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Histopathology and Immunohistochemical Expression of N-Methyl-N-Nitrosourea (NMU) Induced Mammary Tumours in Sprague-Dawley Rats

Cited by 9 publications

References 20 publications

Synergistic Effect of Quercetin Magnetite Nanoparticles and Targeted Radiotherapy in Treatment of Breast Cancer

Synergistic Effect of Quercetin Magnetite Nanoparticles and Targeted Radiotherapy in Treatment of Breast Cancer

Impact of Attenuation Correction on Quantification Accuracy in Preclinical Whole-Body PET Images

Effect of natural nanoparticles products on hepato-renal functions in breast carcinoma female rats

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