Histopathological study of the kidney after administration of methanolic extract of roots of &amp;lt;i&amp;gt;Leptadenia hastata&amp;lt;/i&amp;gt; in the Albino Wistar rat

Diallo, Mama Sy; Bah, Fatoumata; Dial, Chérif; Diop, Cheikh; Faye, Oumar; Seck, Matar

doi:10.4236/ojpathology.2020.102007

Cited by 1 publication

(1 citation statement)

References 8 publications

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“…At the renal level, the lesions observed were a retraction of the flocculus in the glomerular chamber and a discrete vacuolization of the tubular epithelium which could be at the origin the elevation of the creatinine level. These observations have been also found previously by Diallo, et al [26,27].…”

Section: Functional and Anatomopathological Disorders: Liver Kidneys ...supporting

confidence: 90%

In vitro and in vivo Evaluation of the Toxicity Roots of Leptadeniahastata (PER) Decne. Cytotoxicity on Caco2 and HepG2 Cells, Acute and Subacute Toxicity on Wistar Rats

Fatoumata¹,

Aimée²,

Sy³

et al. 2022

J Toxicol Risk Assess

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Background : Nowadays, the use of medicinal plants, for treatment of several pathologies, continues to gain grounds throughout the world. Leptadeniahastata, like other medicinal plants, isused as therapeutic agent for several pathological conditions including diabetes mellitus, diarrhoea, and prostatitis, among other, however, limited data on its toxicity are available.This study aims to evaluate the safety of the L. hastata root extract by in vitro and in vivo tests.Methods: Cytotoxicity and possible apoptosis on Caco 2 and HepG 2 cells were assessed by MTT, Neutral Red, LDH activity and DNA fragmentation assays. The in vivo study was carried out in albino Wistar rats by administering the methanolic extract of L. hastata by gavages, followed by determination of the Lethal Dose 50 (LD50), and the No Observable Adverse Effect Level (NOAEL) to get an idea of its acute and subacutetoxicity. Results:The current study showed a low cytotoxicity effect with an IC 50 greater than 200 μg/mL regardless of cell type and without apoptosis. The acute toxicity of L.hastata's roots is practically low since the LD 50 is greater than 2000 mg/kg. However, the results showed a sub-acute toxicity that manifested itself in deaths and disorders of the digestive system, particularly of the stomach. Although mortality of animals were recorded from the first week as well as inflammation and mutilation of the limbs. Biological and histological changes in the liver and kidneys were also observed. The histological changes detected in the liver were mainly necrotic areas, but also hepatic degeneration, microangiopathy lesions and neutrophil infiltrate. In the kidney, minimal tubular vacuolation and glomerular retraction were observed. The stomach showed only epithelial scaling. The NOAEL was estimated to be 100 mg/ kg for our study. Conclusion:Even though medicinal plants are generally considered to be safe; however, mispractice in its use could be harmful to health; as effects observed could be attributed to the action of L. hastata roots' extracts.

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Section: Functional and Anatomopathological Disorders: Liver Kidneys ...supporting

confidence: 90%

In vitro and in vivo Evaluation of the Toxicity Roots of Leptadeniahastata (PER) Decne. Cytotoxicity on Caco2 and HepG2 Cells, Acute and Subacute Toxicity on Wistar Rats

Fatoumata¹,

Aimée²,

Sy³

et al. 2022

J Toxicol Risk Assess

View full text Add to dashboard Cite

show abstract

Histopathological study of the kidney after administration of methanolic extract of roots of <i>Leptadenia hastata</i> in the Albino Wistar rat

Cited by 1 publication

References 8 publications

In vitro and in vivo Evaluation of the Toxicity Roots of Leptadeniahastata (PER) Decne. Cytotoxicity on Caco2 and HepG2 Cells, Acute and Subacute Toxicity on Wistar Rats

In vitro and in vivo Evaluation of the Toxicity Roots of Leptadeniahastata (PER) Decne. Cytotoxicity on Caco2 and HepG2 Cells, Acute and Subacute Toxicity on Wistar Rats

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