Histopathological study of porcine gastric mucosa with and without a spiral bacterium ("Gastrospirillum suis")

Mendes, Eulália; Queiroz, Dulciene Maria Magalhães; Rocha, Gifone A.; Nogueira, Ana Margarida Miguel Ferreira; Carvalho, A. C. T.; Lage, Andrey Pereira; Barbosa, A. J. A.

doi:10.1099/00222615-35-6-345

Cited by 52 publications

(46 citation statements)

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“…We also tried to culture the bacteria using various culture media under several conditions. However, the spiralled bacteria were not cultured, which is consistent with other reports [8,13].…”

Section: Discussionsupporting

confidence: 91%

“…The histological findings were similar to those reported in man and gnotobiotic piglets colonized by H. pylori [5,11]. There was also a significant association between the presence of Gastrospirillum suis and lymphoid follicles [8]. In some G. suispositive pigs, the fundic mucosa was normal despite the presence of pyloric gastritis.…”

Section: Discussionsupporting

confidence: 86%

“…Mendes et al [8] reported that tightly spiraled bacteria were seen in the pyloric mucosa of the stomach of 13 (10.8%) of 120 pigs and in the fundic mucosa of three (5.0%) out of 60 pigs. In this study, the tightly spiraled bacteria were observed only in the pyloric mucosa of the stomach of 4 (8.0%) of 50 pigs that appeared clinically healthy at slaughter.…”

Section: Discussionmentioning

confidence: 99%

“…Sci. 62 (7): [725][726][727][728][729] 2000 "Gastrospirillum suis", a tightly spiral bacterium, colonizes the gastric mucosa of swine [1,4,7,8]. By 16S rRNA sequencing, the bacterium is known to belong to the genus Helicobacter and is 99.5% similar to "G. hominis" type 1 [3,6].…”

mentioning

confidence: 99%

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Association of Tightly Spiraled Bacterial Infectrion and Gastritis in Pigs.

Park

Lee

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. Tightly spiral bacteria were observed only in the pyloric mucosa of 4 (8.0%) of 50 swine stomachs, mainly in the surface of epithelia, the gastric pits and the lumen of gastric glands. The presence of the spiral bacteria was significantly associated with chronic pyloric gastritis (p<0.05). Mean gastritis score of the bacteria-positive pyloric mucosa was 3.25 ± 0.25, whereas that of the bacterianegative pyloric mucosa was 2.37 ± 0.12. Parakeratosis and hyperkeratosis were spontaneously seen in the mucosa layer of pars oesophagea, regadless of the bacterial infection. Marked infiltration of mononuclear cells and granulocytes were seen in the cardiac mucosa, regardless of the bacterial infection. Mean gastritis score of the bacteria-positive cardiac mucosa was 3.27 ± 0.32, whereas that of the bacteria-negative cardiac mucosa was 2.84 ± 0.13. There was no significant difference between the bacteria-positive and negative cardiac mucosa (p>0.05). Inflammatory response in the fundic mucosa was rare (gastritis score=0.75 ± 0.08). The tightly spiraled bactera were not cultured with various culture media. These results suggest that the presence of tightly spiraled bacteria is associated with only the pyloric gastritis in pigs.KEY WORDS: chronic gastritis, gastric mucosa, swine, tightly spiraled bacteria.J. Vet. Med. Sci. 62 (7): [725][726][727][728][729] 2000 "Gastrospirillum suis", a tightly spiral bacterium, colonizes the gastric mucosa of swine [1,4,7,8]. By 16S rRNA sequencing, the bacterium is known to belong to the genus Helicobacter and is 99.5% similar to "G. hominis" type 1 [3,6]. A possible link between such bacteria and gastric diseases of pig has been evoked [1,12]. Such diseases contribute an important problem in veterinary medicine leading to significant economic consequences, with up to 2.5% of mortality in pigs due to gastrointestinal hemorrhage [14]. Ulceration of the gastric pars esophagus is also a common problem in intensive pig production [1,12]. Several factors have been suspected in the pathogenesis of these lesions, but the etiology still remains unknown.In the present report, we investigated naturally occurring swine gastritis in Korea. In addition, the gastritis scores in the cardiac, fundic, and pyloric mucosa of swine stomach were calculated and analyzed for an association of the gastritis with the tightly spiraled bacterial infection. MATERIALS AND METHODSSample preparation: Stomach samples were obtained from 50 apparently healthy pigs (about 5 months old) weighing about 100-110 kg after slaughter at a slaughterhouse in Suwon, Korea. The stomach samples were transported to the laboratory in individual ice box (4°C) and cut open along the greater curvature from the pylorus to the diverticulum, and the contents were discarded. The stomachs were then washed gently in tap water, taking care to remove food particles only. The mucosal surfaces were examined and assessed for macroscopic lesions.Histopathological and microbiological examination: Fragments of the pars esophagea, cardia, fu...

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Association of Tightly Spiraled Bacterial Infectrion and Gastritis in Pigs.

Park

Lee

et al. 2000

The Journal of Veterinary Medical Science

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“…Studies on this subject are scarce, partly because of the lack of an appropriate animal model. Swine are frequently colonised by a spiral bacterium that was recently shown to belong to the Helicobacter genus by 16S ribosomal RNA sequencing and corresponds to H. heilmannii type 1 (Hh1), a potential human pathogen [11,12]. These spiral bacteria have been observed in the gastric mucosa of patients with gastric symptoms and presenting with histologically proven gastritis [13].…”

Section: Introductionmentioning

confidence: 99%

Increased gastric emptying induced by Helicobacter heilmannii type 1 infection in rats

Duval-Araujo¹,

Queiroz

Magnago

et al. 2000

Journal of Medical Microbiology

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The association between Helicobacter pylori infection and gastric motility abnormalities is still controversial, partly because of the lack of an appropriate animal model. H. heilmannii type 1 (Hh1), a spiral bacterium that infects the stomach of both man and pigs, easily colonises and induces an in¯ammatory response in the gastric mucosa of rodents. For these reasons, the present study investigated the relationship between gastric motility in rats experimentally infected with Hh1 and correlated the results with serum gastrin and gastric somatostatin concentrations, as these hormones seem to be involved in gastric motility. Ten rats were inoculated with gastric mucus from an Hh1-positive pig and 10 animals with gastric mucus from an Hh1-negative pig (control group). After 56 days, gastric emptying was studied in vivo by scintigraphy. The animals were then killed, blood samples were collected for serum gastrin measurement, strips of the gastric wall were obtained for an in-vitro motor study and fragments of the gastric antrum were obtained for somatostatin content evaluation, Hh1 diagnosis and histological study. There was a signi®cant increase in gastric emptying in the test group compared with the controls as demonstrated by the in-vivo and in-vitro studies. Serum gastrin levels were signi®cantly higher and somatostatin levels were lower in the test group than in the controls. In addition, infected animals showed evidence of gastritis on histological examination. Gastric motility is altered in rats infected with Hh1, a fact possibly related to concurrent abnormalities of gastrin and somatostatin secretion.

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