Histopathological Study of Endoscopic Biopsies

Thapa, Rupendra; Lakhey, M; Yadav, Pradeep Kumar; Kandel, Prakash; Aryal, Choodamani; Subba, Kamana

doi:10.31729/jnma.2116

Cited by 13 publications

(22 citation statements)

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“…Majority of cases were inflammatory or benign in nature and chronic non-specific esophagitis (53.33%) was the commonest diagnosis. All neoplastic cases (20%) were squamous cell carcinoma, similar to studies done by Krishnappa R et al 5 , Abilash SC et al 10 and Sheikh BA et al 11 In our study, gastric biopsies constituted the majority of cases (90.12% Thapa R et al 13,14 Twenty seven cases of gastric malignancies were diagnosed on histopathology as gastric adenocarcinoma in line with other studies (Table: 6). The common site of involvement was antrum of the stomach similar as in the other studies.…”

Section: Discussionsupporting

confidence: 90%

Histopathological spectrum of upper gastrointestinal endoscopic biopsies

Hirachand

Sthapit

Gurung

et al. 2018

Jnl. BP Koirala Inst. Health Sci.

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Background: Upper gastrointestinal tract disorders are one of the most commonly encountered problems in the clinical practice. A variety of disorders can affect the upper gastrointestinal tract. Endoscopy, in combination with biopsy, plays an important role in the exact diagnosis for further management.Objectives: To determine the spectrum of histopathological lesions of upper gastrointestinal tract.Methods: A prospective study was conducted in the Department of Pathology, Kathmandu Medical College and Teaching Hospital, Nepal from January 2015 to December 2016 (2 years).Results: A total 243 endoscopic biopsies were evaluated. Out of which, 219 cases were from gastric, 15 were from esophagus and 9 were from duodenum. Among the gastric biopsies, 77 cases (35.16%) were chronic active gastritis and 27 cases (12.33%) were malignant. The most common malignancy was adenocarcinoma. Among the 15 oesophageal biopsies, 12 cases (80%) were of non-neoplastic and 3 cases (20%) were of neoplastic nature. The most common malignancy was squamous cell carcinoma. Among 9 cases of duodenum biopsies, all were non-neoplastic, of which chronic non-specific duodenitis (66.66%) was the commonest.Conclusion: Endoscopy is incomplete without histopathological examination of biopsy and so, the combinations of methods play an important role in diagnosis and management of upper gastrointestinal tract disorders.Journal of BP Koirala Institute of Health Sciences, Vol. 1, No. 1, 2018, Page: 67-74

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Section: Discussionsupporting

confidence: 90%

Histopathological spectrum of upper gastrointestinal endoscopic biopsies

Hirachand

Sthapit

Gurung

et al. 2018

Jnl. BP Koirala Inst. Health Sci.

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“…We got 25 cases of chronic gastritis from 55 gastric biopsies, of which superficial inflammation was seen in 5 cases (9.09%) which is comparable with the study by Thapa et al 2013 [12] . Among the non-neoplastic lesions (30 cases), highest number of cases in gastritis fell under the group of chronic non-specific gastritis accounting for 40% cases.…”

Section: Discussionsupporting

confidence: 87%

Comparative Study of Platelet Indices in Normal Pregnant and Non-Pregnant Women in a Tertiary Care Hospital in Northern India

Somani

Patil

2018

APALM

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This study was conducted to determine the frequency as well as to study the spectrum of the histopathological lesions of upper GI tract. The clinical symptoms, endoscopic findings, and histopathological findings were also correlated. Methods All the details of patients like age, sex, presenting symptoms, site of biopsy, and endoscopic findings were retrieved from the computer database of the hospital. Endoscopies were performed using a large channel flexible endoscope Pentax EG-2770K with EPK-i processor attached to SONY Television screen (monitor) and biopsies were taken by double-bite biopsy forceps equipped with a needle-spike. These biopsies were received in properly labeled and tightly closed container containing 10% formalin, which were then examined grossly for the number and appearance. After adequate fixation, entire biopsy was processed routinely and embedded in paraffin with mucosal surface uppermost. Five micron thick sections were cut perpendicular to this surface using rotary microtome and 3-4 serial sections were prepared on each slide. Sections were stained with routine Hematoxyline and Eosin stain, and mounted with cover slips using Distyrene Plasticizer Xylene (DPX) as mountant. Special stains were done wherever necessary like periodic-Schiff (PAS) and Gomori's methanamine silver (GMS) stain for fungus, reticulin stain to judge the degree of atrophy in gastric mucosa, and Giemsa stain for identification of organisms like Helicobacter pylori. Results The upper GI endoscopic biopsies constituted 0.93% of all surgical pathology specimens received during the period of study. Amongst all the biopsies received, maximum (55%) were from stomach, followed by esophagus (39%) and then, duodenum (6%). Though upper GI lesions are found in almost all the age groups, in the present study, it was seen that maximum biopsies (80%) were obtained from

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“…Although it was varied between studies (16.3–70.5 %) [14–19], we confirmed the prevalence of H. pylori infection is 38.4 % (56/146) using several diagnostic test that significantly related to source of drinking water [20]. The majority of strains are so-called Western-type- cagA in Nepal as similar to typical South Asian patterns [20].…”

Section: Introductionsupporting

confidence: 74%

Emerging Helicobacter pylori levofloxacin resistance and novel genetic mutation in Nepal

et al. 2016

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BackgroundThe prevalence of Helicobacter pylori antibiotic susceptibility in the Nepalese strains is untracked. We determined the antibiotic susceptibility for H. pylori and analyzed the presence of genetic mutations associated with antibiotic resistance in Nepalese strains.ResultsThis study included 146 consecutive patients who underwent gastroduodenal endoscopy in Kathmandu, Nepal. Among 42 isolated H. pylori, there was no resistance to amoxicillin and tetracycline. In contrast, similar with typical South Asian patterns; metronidazole resistance rate in Nepalese strains were extremely high (88.1 %, 37/42). Clarithromycin resistance rate in Nepalese strains were modestly high (21.4 %, 9/42). Most of metronidazole resistant strains had highly distributed rdxA and frxA mutations, but were relative coincidence without a synergistic effect to increase the minimum inhibitory concentration (MIC). Among strains with the high MIC, 63.6 % (7/11) were associated with frameshift mutation at position 18 of frxA with or without rdxA involvement. However, based on next generation sequencing data we found that one strain with the highest MIC value had a novel mutation in the form of amino acid substituted at Ala-212, Gln-382, Ile-485 of dppA and Leu-145, Thr-168, Glu-117, Val-121, Arg-221 in dapF aside from missense mutations in full-length rdxA. Mutations at Asn-87 and/or Asp-91 of the gyrA were predominantly in levofloxacin-resistant strains. The gyrB mutation had steady relationship with the gyrA 87–91 mutations. Although three (44.4 %) and two (22.2 %) of clarithromycin resistant strains had point mutation on A2143G and A2146G, we confirmed the involvement of rpl22 and infB in high MIC strains without an 23SrRNA mutation.ConclusionsThe rates of resistance to clarithromycin, metronidazole and levofloxacin were high in Nepalese strains, indicating that these antibiotics-based triple therapies are not useful as first-line treatment in Nepal. Bismuth or non-bismuth-based quadruple regimens, furazolidone-based triple therapy or rifabutin-based triple therapy may become alternative strategy in Nepal.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0873-6) contains supplementary material, which is available to authorized users.

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Histopathological Study of Endoscopic Biopsies

Cited by 13 publications

References 0 publications

Histopathological spectrum of upper gastrointestinal endoscopic biopsies

Histopathological spectrum of upper gastrointestinal endoscopic biopsies

Comparative Study of Platelet Indices in Normal Pregnant and Non-Pregnant Women in a Tertiary Care Hospital in Northern India

Emerging Helicobacter pylori levofloxacin resistance and novel genetic mutation in Nepal

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