Histopathological Study Of Different Organs Of Charles Foster Rats Under The Exposure Of Pueraria tuberosa

Abstract

Objective: The present study was undertaken to investigate the safe doses of Pueraria tuberosa water extract (PTWE) on different organs .Methodology: Haematoxylin and eosin staining was used to study the morphological alterations in heart, intestine, testis, adrenal gland and spleen. Followed the OECD guidelines 407 of repeated toxicity with respect to the selection of dose and days for different organs. The selected doses of PTWE were 250, 500, 1000 and 2000 mg/kg bw and the durations selected were 7, 14, 21 and 28 days. Result:According to the obtained results, we have found no any types of adverse alteration in cardiac fibers of the heart, size and shapes in crypts and villi of intestine, semeniferous tubules and spermatozoa count were normal in testis, all three zones of adrenal gland were normally identified and no any adverse sign of pulps in spleen was seen in all treated groups of PTWE Conclusion:There was no any types of adverse morphological alteration found in any organs. The drug PTWE are safe at 1000 mg/kg bw upto 28 days and 2000 mg/ kg bw upto 21 days respectively.

Pueraria tuberosa: A Review on Traditional Uses, Pharmacology, and Phytochemistry

“…Aqueous tuber extract of the plant showed an elevated expression of nephrin and SOD and a declined expression of cysteinyl aspartate specific proteinase 3 (caspase-3), interleukin 6 (IL-6), nuclear factor kappa B (NF-κB), protein kinase C epsilon type (PKCε), tumor necrosis factor alpha (TNF-α), vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), and hypoxia-inducible factor 1-alpha in STZ-induced diabetic rats ( Srivastava et al, 2019 ). In another experiment, it has been shown that administration of P. tuberosa water extract in alloxan-induced rat diabetic model resulted in decrease in SGOT (serum glutamic oxaloacetic transaminase), SGPT (serum glutamic pyruvic transaminase), and alkaline phosphates level and improved deformed hepatocytes and significant decrease in blood glucose levels as well as apoptosis ( Pandey et al, 2019 ). The tuber extract contains different bioactive compounds that may act as agonists on glucagon-like peptide-1 hormone released from intestine and can also protect β-cells of the pancreas.…”