Histopathological Studies of Experimentally Infected Shing, <i>Heteropneustes fossilis</i>with <i>Aeromonas hydrophila</i> Bacteria

Abstract

Heteropneustes fossilis (shing) of 35g body weight were experimentally infected with Aeromonas hydrophila by two different methods: viz. intraperitonial and intramuscular injection. A standard dose of infection (6.4 × 10 7 CFU/ml) was selected based on predetermined LD 50 . Each method gave rise to the mortality of shing up to 85%. Clinical signs of fish included injury, hemorrhage and large ulcerative lesions on the body at the injected area. In kidney, liver and intestine pathologically, massive atrophy and focal necrosis were found. Hemorrhage, Vacuolation and atrophy of hepatic sinusoids represented by necrosis of the sinusoidal lining cells, degeneration of hepatic tissue and distribution of bacterial cell all over the tissue were found in liver. Atrophy, hemorrhage, villi missing and missing of epithelium were found in intestine of the experimentally infected shing. Bacterial cells were distributed in the whole hematopoietic tissue including the renal tubules. Tissue abscess characterized by focal necrosis, hemorrhage and Vacuolation were also found in the kidney of the infected shing. But the above symptoms were not found in the organs of the apparently healthy shing species.

ANTIBIOTIC RESISTANT MOTILE AEROMONADS INDUCED SEPTICEMIA IN PHILIPPINE CATFISH Clarias batrachus (Linnaeus, 1758) FINGERLINGS

“…Focal necrosis and cellular degeneration were observed in the kidney of A. hydrophila infected Pangasianodon hypophthalmus (Ly et al, 2009, Faruk et al, 2012. In contrast, Islam et al (2008) noted diffuse necrosis along with massive atrophid hematopoietic tissue in A. hydrophila infected Heteropneustes fossilis. Studies have also documented the presence of melano-macrophage centres in P. hypophthalmus (Faruk et al, 2012) and in estuarine catfish Arius maculates (Alagappan et al, 2009) during A. hydrophila infection, which was not observed in the present study.…”

Characterization of Histopathological and Ultrastructural Changes in Channel Catfish Experimentally Infected with Virulent Aeromonas hydrophila

“…There have been a few histopathological studies of A. hydrophila in catfish ( Bach et al, 1978 ; Grizzle and Kiryu, 1993 ; Alagappan et al, 2009 ; Islam et al, 2013 ). These studies have focused mostly on clinical signs and description of internal lesions after experimental injection of fish.…”

Pathogenicity of the bacterial isolate Aeromonas hydrophila to catfishes, carps and perch

“…Mostofa et al (2008) studied experimental pathogenesis of A. hydrophila bacteria in the same fish. Islam et al (2008) studied histopathological changes in experimentally infected shing with the same bacteria. Lately the bacteria A. hydrophila was isolated from Thai pangus Pangasianodon hypophthalmus (Siddik, 2009) ) and from carps Labeo rohita, Catla catla and Cirrhinus cirrhosus, perch Anabas testudineus, catfishes Heteropneustes fossilis and Clarias batrachus and eel Mastacembalus armatus (Ahmed, 2009).…”