Histopathological, immunohistochemical, and image analytic parameters characterizing the stromal component in primary and recurrent giant cell tumor of bone

Saxena, Charu Chandra; Safaya, Rajni; Madan, Neha Kawatra; Khan, Shah Alam; Iyer, Venkateswaran K.

doi:10.1016/j.jcot.2015.09.006

Cited by 7 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the neoplastic cells, which demonstrated high mitotic figures, are mononuclear. Moreover, CGCGs do not reveal, unlike GCT, high stromal cellularity or necrosis [16].…”

Section: Discussionmentioning

confidence: 81%

Idle gossip about validity of intralesional steroid injection in treating central giant cell granuloma

Alerraqi¹

2016

Horm Stud

View full text Add to dashboard Cite

Treating central giant cell granuloma (CGCG) via intralesional injection of steroids was proven effective in some cases. Later, clinicians have preferred this treatment modality to surgical intervention for it avoids gnathic/bony disfigurement. Advocating this non-surgical therapy, several papers and reviews have recommended putting it into action as the first line of treatment, alone or combined with other treatment options, especially in treating aggressive CGCG. Other authors have reported inaccurate information to validate this approach. This paper scrutinizes, in retrospect, the infelicities about treating CGCG via intralesional injection of steroids and concludes that intralesional steroid injection is useful ONLY in treating non-aggressive CGCG and peripheral giant cell granulomas.

show abstract

“…However, the neoplastic cells, which demonstrated high mitotic figures, are mononuclear. Moreover, CGCGs do not reveal, unlike GCT, high stromal cellularity or necrosis [16].…”

Section: Discussionmentioning

confidence: 81%

Idle gossip about validity of intralesional steroid injection in treating central giant cell granuloma

Alerraqi¹

2016

Horm Stud

View full text Add to dashboard Cite

show abstract

“…All sections were counterstained with hematoxylin. Specific expression markers were examined and photographed using a light microscope, and color densities were quantified using the ImageJ software as described elsewhere [22, 23].…”

Section: Methodsmentioning

confidence: 99%

Flemingia philippinensis Flavonoids Relieve Bone Erosion and Inflammatory Mediators in CIA Mice by Downregulating NF-κB and MAPK Pathways

Sun

Xing

Zeng

et al. 2019

Mediators of Inflammation

View full text Add to dashboard Cite

Background. The dry root of Flemingia philippinensis has been widely used in the treatment of rheumatism, arthropathy, and osteoporosis in traditional Chinese medicine; the therapeutic effects of Flemingia philippinensis are associated with antiarthritis in traditional Chinese medicine theory. This study was undertaken to investigate the mechanism of bone erosion protection and anti-inflammatory effect of Flemingia philippinensis flavonoids (FPF) in collagen-induced arthritis (CIA) in mice. Methods. Flavonoids were extracted from the dry root of Flemingia philippinensis. Collagen-induced arthritis in C57BL/6 mice was used as a rheumatoid arthritis model, and the mice were orally fed with FPF prior to induction to mimic clinical prophylactic therapy for a total of 39 days. After treatment, histology and immunohistochemistry staining were performed, and the levels of anti-collagen type II (CII) antibody and inflammatory mediators, as well as the key proteins of nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, were detected in the samples taken from ankle joints, plasma, and paws. Results. FPF administration significantly suppressed the paw swelling and arthritic score in CIA mice. FPF reduced inflammatory infiltration and pannus formation, articular cartilage destruction and osteoclast infiltration, and the expression of MMP-9 and cathepsin K in the ankle joint. FPF inhibited plasma anti-CII antibody levels and the production of inflammatory cytokines and chemokines in CIA paws. FPF treatment suppressed the activation of NF-κB as indicated by downregulating the phosphorylation of NF-κB p65 and mitogen-activated protein kinases in CIA paws. Additionally, FPF significantly inhibited inflammation signaling by suppressing the activation of activator protein-1 subset and signal transducers and activators of transcription 3 (STAT3). Conclusions. Our data suggest that FPF might be an active therapeutic agent for rheumatoid arthritis and the preventive effect of FPF on arthritis is attributable to an anti-inflammatory effect on CIA by preventing bone destruction, regulating inflammatory mediators, and suppressing NF-κB and MAPK signaling pathways.

show abstract

“…The expression level of mTOR, p-mTOR, AKT, p-AKT, GSK-3β and p-GSK-3β proteins in rat brain tissue was measured by IHC (Saxena et al, 2016;Sun et al, 2019). Briefly, sections of brain tissue were dewaxed and rehydrated.…”

Section: Immunohistochemistry (Ihc) Assaymentioning

confidence: 99%

Baicalin attenuated substantia nigra neuronal apoptosis in Parkinson's disease rats via the mTOR/AKT/GSK-3β pathway

2019

Journal of Integrative Neuroscience

View full text Add to dashboard Cite

This focus of our research is to investigate the protective effect of Baicalin on apoptosis and mTOR/AKT/GSK-3β pathway in substantia nigra neurons in a rat model for Parkinson's disease, induced by 6-Hydroxydopamine. Thirty healthy female Sprague-Dawley rats were randomly divided into control group, model group, and Baicalin group. The Parkinson model was established by injecting 6-Hydroxydopamine into the right substantia nigra of rats in model and Baicalin group. The rats in Baicalin group were intragastrically administered with Baicalin (25 mg/kg/day) for four weeks. At the same time, the rats in control and model groups were intragastrically administered with equivalent solvents. We observed the rat turns, rotation speed and left forelimb usage. The protein expression levels of α-SYN, mTOR, AKT, and GSK-3β in substantia nigra were detected by immunohistochemistry and Western blotting. Compared with model group, Baicalin significantly reduced the number of rotation speeds and neuron apoptosis (P < 0.001, respectively). However, the left forelimb use rate was notably increased after treatment with Baicalin (P < 0.001, respectively). Also, Baicalin decreased the expression levels of α-SYN, mTOR, AKT, and GSK-3β in rats when compared with those in model group (P < 0.001, respectively).

show abstract

Histopathological, immunohistochemical, and image analytic parameters characterizing the stromal component in primary and recurrent giant cell tumor of bone

Cited by 7 publications

References 20 publications

Idle gossip about validity of intralesional steroid injection in treating central giant cell granuloma

Idle gossip about validity of intralesional steroid injection in treating central giant cell granuloma

Flemingia philippinensis Flavonoids Relieve Bone Erosion and Inflammatory Mediators in CIA Mice by Downregulating NF-κB and MAPK Pathways

Baicalin attenuated substantia nigra neuronal apoptosis in Parkinson's disease rats via the mTOR/AKT/GSK-3β pathway

Contact Info

Product

Resources

About