Histopathological image analysis of chemical-induced hepatocellular hypertrophy in mice

Asaoka, Yoshiji; Togashi, Yuko; Mutsuga, Mayu; Imura, Naoko; Miyoshi, Takefumi; Miyamoto, Yohei

doi:10.1016/j.etp.2015.12.005

Cited by 16 publications

(29 citation statements)

References 33 publications

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“…By integrating the RBX technology, cross‐polarized‐mode processing of subsurface red and brown features enables visualization of conditions such as rosacea, spider veins, melasma, and acne. Image‐Pro Plus (IPP ® ) is an image analysis software that has been widely used in many fields such as oral science, ophthalmology, pathology, and dermatology . Recently, IPP ® has been introduced into the field of beauty and cosmetics.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of two kinds of skin imaging analysis software: VISIA^® from Canfield and IPP^® from Media Cybernetics

Wang

Shu

et al. 2018

Skin Research and Technology

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Section: Discussionmentioning

confidence: 99%

“…Plus (IPP ® ) is an image analysis software that has been widely used in many fields such as oral science, 15,16 ophthalmology, 17 pathology, 18,19 and dermatology. 20 Recently, IPP ® has been introduced into the field of beauty and cosmetics.…”

Section: Correlations Between Parameters Analyzed By the Software Amentioning

confidence: 99%

Comparison of two kinds of skin imaging analysis software: VISIA^® from Canfield and IPP^® from Media Cybernetics

Wang

Shu

et al. 2018

Skin Research and Technology

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“…Marcos et al (2016) quantified hepatocytes, Kupffer cells and hepatic stellate cells in the rat liver, thereby combining the advantages of stereology with cytometric analysis of cell ploidy. However, other methods and techniques have also been used for the quantification of hepatocytes, namely, automated image analysis, cell proliferation assays, protein concentration measurements, DNA content methods and cytochrome P450 content methods (Carlile et al 1997;Stegemann et al 2000;Haga et al 2005;Sohlenius-Sternbeck, 2006;Deng et al 2009;Miyaoka et al 2012;Garrido et al 2013;Best et al 2015;Asaoka et al 2016). Many of these techniques are based on the use of single cell suspensions or liver homogenates.…”

Section: Histological Quantification Of Hepatocytesmentioning

confidence: 99%

Stereological analysis of size and density of hepatocytes in the porcine liver

et al. 2016

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The porcine liver is frequently used as a large animal model for verification of surgical techniques, as well as experimental therapies. Often, a histological evaluation is required that include measurements of the size, nuclearity or density of hepatocytes. Our aims were to assess the mean number-weighted volume of hepatocytes, the numerical density of hepatocytes, and the fraction of binuclear hepatocytes (BnHEP) in the porcine liver, and compare the distribution of these parameters among hepatic lobes and macroscopic regions of interest (ROIs) with different positions related to the liver vasculature. Using disector and nucleator as design-based stereological methods, the morphometry of hepatocytes was quantified in seven healthy piglets. The samples were obtained from all six hepatic lobes and three ROIs (peripheral, paracaval and paraportal) within each lobe. Histological sections (thickness 16 μm) of formalin-fixed paraffin-embedded material were stained with the periodic acid-Schiff reaction to indicate the cell outlines and were assessed in a series of 3-μm-thick optical sections. The mean number-weighted volume of mononuclear hepatocytes (MnHEP) in all samples was 3670 ± 805 μm (mean ± SD). The mean number-weighted volume of BnHEP was 7050 ± 2550 μm . The fraction of BnHEP was 4 ± 2%. The numerical density of all hepatocytes was 146 997 ± 15 738 cells mm of liver parenchyma. The porcine hepatic lobes contained hepatocytes of a comparable size, nuclearity and density. No significant differences were identified between the lobes. The peripheral ROIs of the hepatic lobes contained the largest MnHEP with the smallest numerical density. The distribution of a larger MnHEP was correlated with a larger volume of BnHEP and a smaller numerical density of all hepatocytes. Practical recommendations for designing studies that involve stereological evaluations of the size, nuclearity and density of hepatocytes in porcine liver are provided.

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“…It is known that there are substantial intra‐ and interobservation variations between experts (Asaoka et al . ). Computer‐assisted image analysis refers to the field of using computer algorithms to extract quantitative information from digital images of sections (Kårsnäs ).…”

Section: Introductionmentioning

confidence: 97%

“…The analysis of tissue sections depends on the experience and expertise of histopathologists, and it is laborious and difficult to grade in a reproducible manner. It is known that there are substantial intra-and interobservation variations between experts (Asaoka et al 2016). Computerassisted image analysis refers to the field of using computer algorithms to extract quantitative information from digital images of sections (K arsn€ as 2014).…”

mentioning

confidence: 99%

Optimization of fixation methods for image analysis of the hepatopancreas in whiteleg shrimp, Penaeus vannamei (Boone)

Cervellione

McGurk

Silva

et al. 2016

Journal of Fish Diseases

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Pathology in penaeid shrimps relies on histology, which is subjective, time-consuming and difficult to grade in a reproducible manner. Automated image analysis is faster, objective and suitable for routine screening; however, it requires standardized protocols. The first critical step is proper fixation of the target tissue. Bell & Lightner's (A Handbook of Normal Penaeid Shrimp Histology, 1988, The World Aquaculture Society, Baton Rouge) fixation protocol, widely used for routine histology of paraffin sections, is not optimized for image analysis, and no protocol for frozen sections is described in the available literature. Therefore, the aim of this study was to optimize fixation of the hepatopancreas (HP) from whiteleg shrimp (Penaeus vannamei) for both paraffin and frozen sections using a semiquantitative scoring system. For paraffin sections, four injection volumes and three injection methods were compared, for frozen sections, four freezing methods and four fixation methods. For paraffin sections, optimal fixation was achieved by increasing threefold the fixative volume recommended by Bell and Lightner, from 10% to 30% of the shrimp body weight, combined with single injection into the HP. Optimal fixation for frozen sections was achieved by freezing the cephalothorax with liquid nitrogen, followed by fixation of the section with 60% isopropanol. These optimized methods enable the future use of image analysis and improve classical histology.

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Histopathological image analysis of chemical-induced hepatocellular hypertrophy in mice

Cited by 16 publications

References 33 publications

Comparison of two kinds of skin imaging analysis software: VISIA^® from Canfield and IPP^® from Media Cybernetics

Comparison of two kinds of skin imaging analysis software: VISIA^® from Canfield and IPP^® from Media Cybernetics

Stereological analysis of size and density of hepatocytes in the porcine liver

Optimization of fixation methods for image analysis of the hepatopancreas in whiteleg shrimp, Penaeus vannamei (Boone)

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Histopathological image analysis of chemical-induced hepatocellular hypertrophy in mice

Cited by 16 publications

References 33 publications

Comparison of two kinds of skin imaging analysis software: VISIA® from Canfield and IPP® from Media Cybernetics

Comparison of two kinds of skin imaging analysis software: VISIA® from Canfield and IPP® from Media Cybernetics

Stereological analysis of size and density of hepatocytes in the porcine liver

Optimization of fixation methods for image analysis of the hepatopancreas in whiteleg shrimp, Penaeus vannamei (Boone)

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Product

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Comparison of two kinds of skin imaging analysis software: VISIA^® from Canfield and IPP^® from Media Cybernetics

Comparison of two kinds of skin imaging analysis software: VISIA^® from Canfield and IPP^® from Media Cybernetics