Histopathological, genotoxic, and behavioral damages induced by manganese (II) in adult zebrafish

Rodrigues, Gabriela Zimmermann Prado; Staudt, Luciane Beatris Mentges; Moreira, Marina Griebeler; Santos, Thainá Garbino dos; Souza, Mateus Santos de; Lúcio, Caio Júnior; Panizzon, Jenifer; Kayser, Juliana Machado; Simões, Leonardo Airton Ressel; Ziulkoski, Ana Luíza; Bonan, Carla Denise; Oliveira, Diogo Lösch de; Gehlen, Günther

doi:10.1016/j.chemosphere.2019.125550

Cited by 18 publications

(10 citation statements)

References 47 publications

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“…Controlled environmental conditions for laboratory animals provide a basis for successful animal husbandry and welfare and reproducible results. For zebrafish, husbandry studies included the roles of anesthesia and euthanasia, 46 diseases and pathogens, 47,48 behavior, [20][21][22][23]49,50 housing, 39,45,[49][50][51] nutrition, 52,53 and reproduction. [54][55][56] In this study, we explored concentrations of metals and metalloids in different CRWS that are used in biomedical zebrafish research facilities.…”

Section: Discussionmentioning

confidence: 99%

“…Controlled environmental conditions for laboratory animals provide a basis for successful animal husbandry and welfare and reproducible results. For zebrafish, husbandry studies included the roles of anesthesia and euthanasia, 46 diseases and pathogens, 47 , 48 behavior, 20–23 , 49 , 50 housing, 39 , 45 , 49–51 nutrition, 52 , 53 and reproduction. 54–56 …”

Section: Discussionmentioning

confidence: 99%

“…13,14 Conversely, high concentrations can also lead to severe symptoms and disorders in reproduction, growth and development, 13,[15][16][17] metabolism, 10,18,19 and behavior. [20][21][22] The toxicity of particular metals is typically defined by their concentration of free ions in water, which is highly dependent on environmental parameters such as water hardness, conductivity, temperature, [23][24][25][26] and pH. 27,28 Environmental agencies such as the Environmental Protection Agency (EPA, USA) and the Australia, New Zealand Environment, and Conservation Council (ANZECC) developed guidelines for freshwater and saltwater levels for various metals (Table 1).…”

Section: Introductionmentioning

confidence: 99%

“… 13 , 14 Conversely, high concentrations can also lead to severe symptoms and disorders in reproduction, growth and development, 13 , 15–17 metabolism, 10 , 18 , 19 and behavior. 20–22 The toxicity of particular metals is typically defined by their concentration of free ions in water, which is highly dependent on environmental parameters such as water hardness, conductivity, temperature, 23–26 and pH. 27 , 28 …”

Section: Introductionmentioning

confidence: 99%

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A Systematic Analysis of Metal and Metalloid Concentrations in Eight Zebrafish Recirculating Water Systems

et al. 2021

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Metals and metalloids are integral to biological processes and play key roles in physiology and metabolism. Nonetheless, overexposure to some metals or lack of others can lead to serious health consequences. In this study, eight zebrafish facilities collaborated to generate a multielement analysis of their centralized recirculating water systems. We report a first set of average concentrations for 46 elements detected in zebrafish facilities. Our results help to establish an initial baseline for trouble-shooting purposes, and in general for safe ranges of metal concentrations in recirculating water systems, supporting reproducible scientific research outcomes with zebrafish.

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Section: Discussionmentioning

confidence: 99%

“…Controlled environmental conditions for laboratory animals provide a basis for successful animal husbandry and welfare and reproducible results. For zebrafish, husbandry studies included the roles of anesthesia and euthanasia, 46 diseases and pathogens, 47 , 48 behavior, 20–23 , 49 , 50 housing, 39 , 45 , 49–51 nutrition, 52 , 53 and reproduction. 54–56 …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Systematic Analysis of Metal and Metalloid Concentrations in Eight Zebrafish Recirculating Water Systems

et al. 2021

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“…The manganese chloride reagent (MnCl 2 4H 2 O, LabSynth®, 98% purity) was used to prepare a stock solution, which was subsequently diluted in the aquariums to reach concentrations of 0.5 and 4.0 mg L -1 of MnCl 2 (in which each 1 mg L -1 of MnCl 2 corresponds to 0.28 mg L -1 of manganese since manganese represents 27.8% of the mass of MnCl 2 4H 2 0), according to a study previously published (Rodrigues et al 2020). Water changes, both in the acclimatization and exposure and recovery periods, occurred every 48 hours.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of reversal and transposition generational damages caused by manganese (II) in zebrafish

Rodrigues

Santos

Finkler

et al. 2021

Preprint

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Although manganese (Mn) is a common water pollutant, little is known about the potential for the trans-generational transfer of biological effects in fish exposed to low concentrations in aquatic organisms, or the persistence of these effects. The aim was to identify when biological effects were firstly observed in the brain, liver and intestinal tissues of zebrafish exposed to environmentally relevant concentrations of Mn and whether these effects were subject to reversal when exposures ceased. We also aimed to assess whether biological effects observed in zebrafish exposed to Mn for 21-days were also observed in the early life stages of the F1 generation. Behavioral changes were observed in the animals exposed for 14 days to MnCl2 (4.0 mg L-1). There was also an oscillation in the mitochondrial activity and TUNEL positive cells in the telencephalon of exposed fish from all treatments. These results indicated that brain neurogenesis in zebrafish occurred with greater magnitude and speed than the neuronal Mn damage. Histopathological changes were observed in the intestine and liver of zebrafish exposed to Mn for 72 h and 21 days. Behavioral alterations and intestinal histopathological alterations were reversed after the recovery period, although hepatocyte vacuolization persisted. Behavioral alterations were also observed in the early developmental stages of F1 generation larvae of zebrafish parents exposed to Mn, but this profile was normalized throughout the development. In addition to the direct toxic effects of low concentrations of Mn, some changes, such as hepatocyte vacuolization persisted in zebrafish even after the toxicant removal.

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Evaluation of transgenerational effects caused by metals as environmental pollutants in Daphnia magna

Rodrigues

Finkler

Garcia

et al. 2020

Environ Monit Assess

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Histopathological, genotoxic, and behavioral damages induced by manganese (II) in adult zebrafish

Cited by 18 publications

References 47 publications

A Systematic Analysis of Metal and Metalloid Concentrations in Eight Zebrafish Recirculating Water Systems

A Systematic Analysis of Metal and Metalloid Concentrations in Eight Zebrafish Recirculating Water Systems

Evaluation of reversal and transposition generational damages caused by manganese (II) in zebrafish

Evaluation of transgenerational effects caused by metals as environmental pollutants in Daphnia magna

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