Histopathological evaluation of potential impact of β-tricalcium phosphate (HA+ β-TCP) granules on healing of segmental femur bone defect

Abstract

Histopathological evaluation of β-tricalcium phosphate (HA+ β-TCP) granules demonstrated that it has properties to heal segmental femur bone defect in rat. In this study, 27 male white rats were examined. Rats were divided into tree groups. Surgical procedures were done after IP administration of ketamine 5 % and xylazine HCL 2 %. Then an approximately 5-mm long, 3-mm deep and 2-mm wide bone defect was created in the femur of one of the hind limbs using a No. 0.14 round bur. After inducing the surgical wound, all rats were colored and randomly divided into three experimental groups of nine animals each: Group 1 received medical pure β-tricalcium phosphate granules, group 2 received hydroxyapatite and third group was a control group with no treatment. Histopathological evaluation was performed on days 15, 30 and 45 after surgery. On day 45 after surgery, the quantity of newly formed lamellar bone in the healing site in β-TCP group was better than onward compared to HA and control groups. In conclusion, β-tri calcium phosphate (β-TCP) granules exhibited a reproducible bone-healing potential (Fig. 10

Guided bone regeneration with osteoconductive grafts and PDGF: A tissue engineering option for segmental bone defect reconstruction

“…9,37,38 Studies have confirmed the reliability of rat models for reporting qualitative and quantitative histological assessment of bone healing and regeneration, in both membranous (calvarium) and endochondral (femur) bone defects. 8,23,25,33,34,37,39,40 Although the effectiveness of GBR for treating SBD and the adjunctive role of PDGF have not been studied extensively, based on an earlier in-vivo radiographic study, the present authors reported new bone formation in rat femoral SBD after 2-4 weeks of GBR with alloplastic bone substitute and PDGF. 41 This study envisaged assessment of long term bone healing after GBR of segmental defects along with adjunct PDGF.…”

“…21,32 Alloplastic beta-TCP has been used an osteoconductive scaffold for regeneration of small to medium bone defects in orthopedic and dental surgery, and are capable of indirect osteoinductivity through entrapment of growth factors and osteoprogenitor cells. 6,11,39,45 Since beta-TCP resorbs faster than osteoconductive allografts and xenografts, it enables early bone formation within the grafted site and mineralization through hastened release of calcium and phosphorus ions. 8,46 The ability of beta-TCP to enhance GBR and peri-implant bone healing have been reported by several experimental studies.…”

“…8,46 The ability of beta-TCP to enhance GBR and peri-implant bone healing have been reported by several experimental studies. 8,23,24,32,39,47 Using beta-TCP in rat femoral defects, Eftekhari et al reported new bone formation within 4-weeks and significantly greater bone formation than with hydroxyapatite by 8-weeks. 39 While similar results were observed in the present study too, addition of PDGF to beta-TCP resulted in even better qualitative and quantitative bone formation, than with beta-TCP alone (Figures 3, 4 and 5).…”

The Influences of Different Ratios of Biphasic Calcium Phosphate and Collagen Augmentation on Posterior Lumbar Spinal Fusion in Rat Model

“… 39 40 As previously reported, β-TCP is biodegradable and more easily resorbed than HA. 41 In an ideal situation, it is better that a biodegradable bone substitute is slowly resorbed and replaced by natural bone. 41 In cases that percentage of HA particle was excessive, new bone formation within graft materials might be partly inhibited.…”

“… 41 In an ideal situation, it is better that a biodegradable bone substitute is slowly resorbed and replaced by natural bone. 41 In cases that percentage of HA particle was excessive, new bone formation within graft materials might be partly inhibited. 39 40 Therefore, although appropriate BCP ratio was deemed important, it has yet to be determined.…”