Histopathological evaluation of collagen fibers using picrosirius red stain and polarizing microscopy in oral squamous cell carcinoma

Manjunatha, Bhari Sharanesha; Agrawal, Aditi; Shah, Vandana

doi:10.4103/0973-1482.154061

Cited by 28 publications

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“…As well as in our study other authors has used this method in various tissues to quantify collagen, for example Singh and colleagues (2012) [ 52 ] in their study about role of connective tissue stroma on biological behavior of odontogenic cysts, Geary and colleagues (2015) [ 53 ] in study of tendon repair, Manjunatha and colleagues (2015) [ 54 ] in oral squamous cell carcinoma, Alves and colleagues (2015) [ 55 ] in a study of the splenic collagen polymorphism, and many others.…”

Section: Discussionmentioning

confidence: 80%

Structural and Ultrastructural Characteristics of Bone-Tendon Junction of the Calcaneal Tendon of Adult and Elderly Wistar Rats

et al. 2016

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Tendons are transition tissues that transfer the contractile forces generated by the muscles to the bones, allowing movement. The region where the tendon attaches to the bone is called bone-tendon junction or enthesis and may be classified as fibrous or fibrocartilaginous. This study aims to analyze the collagen fibers and the cells present in the bone-tendon junction using light microscopy and ultrastructural techniques as scanning electron microscopy and transmission electron microscopy. Forty male Wistar rats were used in the experiment, being 20 adult rats at 4 months-old and 20 elderly rats at 20 months-old. The hind limbs of the rats were removed, dissected and prepared to light microscopy, transmission electron microscopy and scanning electron microscopy. The aging process showed changes in the collagen fibrils, with a predominance of type III fibers in the elderly group, in addition to a decrease in the amount of the fibrocartilage cells, fewer and shorter cytoplasmic processes and a decreased synthetic capacity due to degradation of the organelles involved in synthesis.

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Section: Discussionmentioning

confidence: 80%

Structural and Ultrastructural Characteristics of Bone-Tendon Junction of the Calcaneal Tendon of Adult and Elderly Wistar Rats

et al. 2016

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“…They concluded that the collagen fibers were more disorganized in moderately to poorly differentiated OSCC. [ 9 ]…”

Section: Discussionmentioning

confidence: 99%

“…Various authors have studied the birefringence property of collagen fibers,[ 3 4 5 ] but studies which evaluated the other aspects of collagen fibers (orientation, arrangement, density, packing and area fraction) in OSCC are only a handful. [ 6 7 8 9 10 ] Apart from collagen fibers, the elastic fibers also are an integral component of the extracellular matrix (ECM). As per the literature, there is an interaction between the tumor cells and elastic fibers, but its effect on the progression of carcinoma is questionable.…”

Section: Introductionmentioning

confidence: 99%

Stromal fibers in oral squamous cell carcinoma: A possible new prognostic indicator?

Kardam¹,

Mehendiratta²,

Rehani³

et al. 2016

J Oral Maxillofac Pathol

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Background:Many studies have been carried out to study the role of extracellular matrix proteins, growth factors and matrix metalloproteinases on tumor invasion. However, literature related to the analysis of connective tissue fibers in varying grades of oral squamous cell carcinoma (OSCC) is very limited.Aim:To analyze the changes in collagen and elastic fibers in varying grades of (OSCC).Settings and Design:This retrospective study was carried out using a light and polarizing microscope.Materials and Methods:Three sections each were cut from fifty samples of varying grades of OSCC and ten samples of control followed by staining with H and E, Picrosirius-Red and Verhoeff–Van Gieson. Qualitative and quantitative analysis of collagen and elastic fibers were accomplished using set criteria.Statistical Analysis:Data were entered into the Statistical Package for Social Sciences (SPSS) version 13.5 for analysis.Results:A change in colors of collagen fibers was seen on progressing from well to poorly differentiated OSCC. Thin collagen fibers predominantly exhibited greenish yellow, but the thick fibers exhibited a variety of colors. As the grade of OSCC progressed, collagen fibers were loosely packed haphazardly arranged. Statistically insignificant results were obtained for quantitative analysis of collagen and qualitative analysis of elastic fibers.Conclusion:The collagen fibers undergo a change in color, orientation and packing in the stroma of varying grades of OSCC. The uniqueness of this study lies in the exploration of elastic fibers in OSCC which has not been done so far.

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“…OSCC is composed of two distinct components: (a) the malignant epithelial cells; and (b) the stroma in which they are dispersed . One of the major aspects of tumor cell invasion and metastasis is the interaction between cancer cells and the extracellular matrix (ECM) component . The ECM is composed of a ground substance made up of proteoglycans, glycoproteins, and water, and the fibrous component, including collagen and elastic fibers .…”

Section: Introductionmentioning

confidence: 99%

Histochemical analysis of collagen reorganization at the invasive front of oral squamous cell carcinoma tumors

Alrani

Acharya

et al. 2017

J of Invest & Clin Dent

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Histopathological evaluation of collagen fibers using picrosirius red stain and polarizing microscopy in oral squamous cell carcinoma

Abstract: An observable stromal change with the progression of neoplasm was evinced with picrosirius red stain in different thickness of collagens with a significant change in the arrangement from the early stage to the advanced stage according to tumorogenesis.

Cited by 28 publications

References 0 publications

Structural and Ultrastructural Characteristics of Bone-Tendon Junction of the Calcaneal Tendon of Adult and Elderly Wistar Rats

Structural and Ultrastructural Characteristics of Bone-Tendon Junction of the Calcaneal Tendon of Adult and Elderly Wistar Rats

Stromal fibers in oral squamous cell carcinoma: A possible new prognostic indicator?

Histochemical analysis of collagen reorganization at the invasive front of oral squamous cell carcinoma tumors

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