Histopathological effects, responses of oxidative stress, inflammation, apoptosis biomarkers and alteration of gene expressions related to apoptosis, oxidative stress, and reproductive system in chlorpyrifos-exposed common carp ( Cyprinus carpio L.)

Altun, Serdar; Özdemir, Selçuk; Arslan, Harun

doi:10.1016/j.envpol.2017.06.085

Cited by 75 publications

(21 citation statements)

References 84 publications

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“…The gene expression results showed an increase in expression of iNOS and NOX4 pro-oxidant genes and a decrease in expression of Gpx antioxidant gene. iNOS and NOX4 play a central role in toxicity of environmental toxicants through catalyzing the production of nitric oxide (NO) and superoxide free radicals (Altun et al, 2017). Consistent with our results, different studies have shown that high expression and activation of iNOS plays a crucial role in testicular dysfunction and apoptosis through overproduction of NO oxidant (Banerjee et al, 2016;Altun et al, 2017).…”

Section: Discussionsupporting

confidence: 90%

“…iNOS and NOX4 play a central role in toxicity of environmental toxicants through catalyzing the production of nitric oxide (NO) and superoxide free radicals (Altun et al, 2017). Consistent with our results, different studies have shown that high expression and activation of iNOS plays a crucial role in testicular dysfunction and apoptosis through overproduction of NO oxidant (Banerjee et al, 2016; Altun et al, 2017). One of the main sources of ROS production in numerous tissue types is NOXs, and the role of NOXs in induction of oxidative stress in different diseases has been shown (Choi et al, 2014; Manea and Simionescu, 2012; Pourgholamhossein et al, 2018; Sedeek et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

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Mancozeb induces testicular dysfunction through oxidative stress and apoptosis: Protective role of N-acetylcysteine antioxidant

et al. 2018

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Mancozeb (MZB) is one of the fungicides used in pest control programs that might affect human health including reproductive system. The aim of this study was to demonstrate the mechanisms through which MZB induces testicular tissue damage and the probable protective effect of N-acetylcysteine (NAC), a modified amino acid, with antioxidant property, against MZB toxicity in an animal model. Male albino mice ( n = 8) were exposed to different doses of MZB (250 and 500 mg/kg/day) by oral gavage without or with NAC (200 mg/kg, twice/week) for 40 days. Sub-chronic MZB dose-dependently decreased sperm motility and count. Exposure to MZB increased lipid peroxidation and protein carbonyl, while it reduced antioxidant enzymes activities, total antioxidant capacity, and glutathione content. The histopathological examination clearly showed deleterious changes in the testicular structure. At the molecular levels, the results of quantitative real time-poly chain reaction (qRT-PCR) showed that MZB upregulated oxidative stress markers inducible nitric oxide synthase (iNOS) and NADPH oxidase 4 (NOX4) and downregulated expression of the glutathione peroxidase 1 (Gpx1) gene as one of the most important antioxidant enzymes. MZB also induced apoptosis dose-dependently in the testes as determined by the terminal dUTP nick-end labeling assay and immunoblotting. NAC administration decreased the mRNA levels of both iNOS and NOX4 with a concomitant increase in Gpx1 expression. It also significantly decreased MZB-induced oxidative stress and apoptosis. Collectively, the present study showed MZB-induced oxidative damage in testes leading to apoptosis. It revealed that antioxidants such as NAC can mitigate oxidant injury induced by the dithiocarbamate pesticides in the reproductive system.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Mancozeb induces testicular dysfunction through oxidative stress and apoptosis: Protective role of N-acetylcysteine antioxidant

et al. 2018

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“…The oxidative damage on exposed and control samples were significantly different (P<0.05); being the carbonyl index 2.7-fold higher in samples exposed to CPF than those used as control. These results showed the capacity of CPF to promote oxidative damage on muscle proteins, which are in accordance with the results observed on myosin protein (section 3.1) and other assays performed in biological models such as rat and fish (Altun, Özdemir, & Arslan, 2017;Owumi & Dim, 2019). Although the mechanism that promotes protein carbonylation induced by CPF is still unknown, it could be related to its high lipophilicity (Kim et al, 2016).…”

Section: Carbonylation Induced By Chlorpyrifos On Chicken Breast Proteinssupporting

confidence: 86%

In vitro oxidation promoted by chlorpyrifos residues on myosin and chicken breast proteins

Lázaro¹,

Mora

Méndez-Cuadro

et al. 2020

Food Chemistry

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Organophosphate pesticides are frequently used to eliminate or prevent insects in poultry. However, their residues may continue in meat after slaughtering. In this study, proteomics and peptidomics approaches were used to evaluate their oxidative impact on myosin and chicken breast proteins under in vitro conditions. Myosin protein was exposed to diazinon and chlorpyrifos showing an increase in its oxidation by increasing times, especially with chlorpyrifos.Then, chicken breast was contaminated with chlorpyrifos to evaluate carbonylation and the effect of simulated gastrointestinal digestion. Proteins were isolated using size-exclusionchromatography and identified by mass spectrometry in tandem. Myosin, β-enolase, CK-M-type and actin were identified as main proteins susceptible to oxidation. Also, oxidised peptides obtained before and after simulated gastrointestinal digestion were identified.Collagen peptides the most susceptible to oxidation. These results suggest that the presence of chlorpyrifos residues on meat could have a negative effect on its final quality and nutritional value.

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“…In the present study common carp, Cyprinus carpio, has been chosen as model species for 129 its economic importance worldwide and its use as bioindicator species in environmental 130 pollution studies due its resistance to heavily polluted habitats (Altun et al 2017, Rajeshkumar 131 et al 2017. Its availability and ease to handle makes it also a suitable species for 132 transplantation studies for micropollutant bioaccumulation (Bervoets et al 2009, Schoenaers 133 et al 2016, Delahaut et al 2019b).…”

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confidence: 99%

Investigating the effects of a sub-lethal metal mixture of Cu, Zn and Cd on bioaccumulation and ionoregulation in common carp, Cyprinus carpio

et al. 2020

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14The aquatic environment is continuously under threat because it is the final receptor and sink 15 of waste streams. The development of industry, mining activities and agriculture gave rise to 16 an increase in metal pollution in the aquatic system. Thus a wide occurrence of metal mixtures 17 exists in the aquatic environment. The assessment of mixture stress remains a challenge 18 considering that we can not predict the toxicity of a mixture on the basis of single compounds.35 common carp are able to cope with these low metal concentrations, at least during a one week 36 exposure.

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Histopathological effects, responses of oxidative stress, inflammation, apoptosis biomarkers and alteration of gene expressions related to apoptosis, oxidative stress, and reproductive system in chlorpyrifos-exposed common carp ( Cyprinus carpio L.)

Cited by 75 publications

References 84 publications

Mancozeb induces testicular dysfunction through oxidative stress and apoptosis: Protective role of N-acetylcysteine antioxidant

Mancozeb induces testicular dysfunction through oxidative stress and apoptosis: Protective role of N-acetylcysteine antioxidant

In vitro oxidation promoted by chlorpyrifos residues on myosin and chicken breast proteins

Investigating the effects of a sub-lethal metal mixture of Cu, Zn and Cd on bioaccumulation and ionoregulation in common carp, Cyprinus carpio

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