Histopathological effect and stress response of mantle proteome following TBT exposure in the Hooded oyster Saccostrea cucullata

Impacts of the combined exposure to seawater acidification and arsenic on the proteome of Crassostrea angulata and Crassostrea gigas

“…This mitochondrial enzyme is an important player in the TCA cycle, and a potential oxidative stress biomarker (Bota et al, 2002). Khondee et al (2016) also observed increased Achd abundance in C. gigas exposed to TBT and explained their results as a stimulation of the energy flow through the TCA cycle to fuel energetically demanding responses towards xenobiotic exposure. Because Achd is sensitive to oxidative stress (Cherkasov et al, 2007), its transcriptional upregulation has also been discussed as a compensation mechanism to counteract ROS-mediated self-inactivation in Argopecten irradians scallops under hypoxia (Ivanina et al, 2016).…”

“…Alterations in the expression levels of cytoskeletal proteins is a common response observed in marine organisms exposed to environmental stress (Tomanek, 2014). Altered levels of Actin have also been reported in other oyster species (S. cucculata and S. glomerata) exposed to different types of contaminants (Muralidharan et al, 2012;Thompson et al, 2012;Khondee et al, 2016;Melwani et al, 2016), and could be indicative of cytoskeleton rearrangement to prevent oxidative damage (Dailianis et al, 2009).…”

“…For instance, increased expression of Severin has been described in C. gigas under thermal and hypercapnic stress (Dineshram et al, 2015;Harney et al, 2016), and in S. glomerata exposed to highly polluted sites (Melwani et al, 2016). Likewise, altered abundance of Gelsolin-like proteins was described in S. cucculata exposed to TBT (Khondee et al, 2016), and Pecten maximus under hypoxia (Artigaud et al, 2015). In addition the importance of cytoskeletal proteins on Actin dynamics regulation, several other biological functions have been linked to Gelsolin superfamily proteins, which include modulation of cell motility, cortical shape changes, apoptosis and phagocytosis (Silacci et al, 2004;Li et al, 2009).…”

“…Hence Aldh is an important enzyme in the oxidative stress response, by mitigating deleterious effects of aldehyde formation in processes such as lipid peroxidation (Marchitti et al, 2008;Singh et al, 2013). Several studies have demonstrated increased Aldh expression in bivalve species in response to environmental stress, including S. cucculata exposed to TBT (Khondee et al, 2016) and M. galloprovincialis under thermal stress (Tomanek, 2012). Apart from aldehyde detoxification, another role of Aldh is to provide reducing equivalents (NADPH) to increase reactive oxygen species (ROS) scavenging capacity by the glutathione system (Tomanek, 2014(Tomanek, , 2015.…”

“…gigas presented higher expression levels of Enolase in Low pH exposure compared to Low pH + As (Table 2). Other studies have described increased expression of Enolase in bivalves, such as Mytilus edulis under thermal stress (Péden et al, 2016) and S. cucculata exposed to TBT (Khondee et al, 2016). Glycolysis stimulation by Enolase has been discussed as a mechanism to increase carbohydrate metabolism in bivalves experiencing energetically demanding conditions (Tomanek, 2014;Artigaud et al, 2015).…”