Histopathological correlation of cartilage swelling detected by magnetic resonance imaging in early experimental osteoarthritis

Calvo, Emílio; Palacios, Itziar; Delgado, Emilio; Sánchez‐Pernaute, Olga; Largo, Raquel; Egido, Jesús; Herrero‐Beaumont, Gabriel

doi:10.1016/j.joca.2004.07.007

Cited by 128 publications

(98 citation statements)

References 31 publications

(30 reference statements)

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“…In all cases, after reaching its maximum z-displacement, the tissue maintains its morphology until the end of the simulation (3600 s; figure 5). It is important to remark that the results obtained are consistent with recent experimental observations where abnormal swelling was detected when the tissue presented the first symptoms of disease [20,55,56].…”

Section: Z-displacementsupporting

confidence: 91%

“…Biologically, when OA progresses the collagen structure suffers a progressive deterioration and a sharp PG loss. Thus, the capacity of the collagen network to retain water is reduced, and the cation flux is clearly overpassed by the intake water flux, which leads to subsequent substrate swelling [20,55]. Note that for normal human knee cartilage, cation fluxes exceed 2 Â 10 25 mol s 21 .…”

Section: Cation Fluxesmentioning

confidence: 99%

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Altered swelling and ion fluxes in articular cartilage as a biomarker in osteoarthritis and joint immobilization: a computational analysis

Manzano

Doblaré

et al. 2015

J. R. Soc. Interface.

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In healthy cartilage, mechano-electrochemical phenomena act together to maintain tissue homeostasis. Osteoarthritis (OA) and degenerative diseases disrupt this biological equilibrium by causing structural deterioration and subsequent dysfunction of the tissue. Swelling and ion flux alteration as well as abnormal ion distribution are proposed as primary indicators of tissue degradation. In this paper, we present an extension of a previous three-dimensional computational model of the cartilage behaviour developed by the authors to simulate the contribution of the main tissue components in its behaviour. The model considers the mechano-electrochemical events as concurrent phenomena in a three-dimensional environment. This model has been extended here to include the effect of repulsion of negative charges attached to proteoglycans. Moreover, we have studied the fluctuation of these charges owning to proteoglycan variations in healthy and pathological articular cartilage. In this sense, standard patterns of healthy and degraded tissue behaviour can be obtained which could be a helpful diagnostic tool. By introducing measured properties of unhealthy cartilage into the computational model, the severity of tissue degeneration can be predicted avoiding complex tissue extraction and subsequent in vitro analysis. In this work, the model has been applied to monitor and analyse cartilage behaviour at different stages of OA and in both short (four, six and eight weeks) and long-term (11 weeks) fully immobilized joints. Simulation results showed marked differences in the corresponding swelling phenomena, in outgoing cation fluxes and in cation distributions. Furthermore, long-term immobilized patients display similar swelling as well as fluxes and distribution of cations to patients in the early stages of OA, thus, preventive treatments are highly recommended to avoid tissue deterioration.

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Section: Z-displacementsupporting

confidence: 91%

Section: Cation Fluxesmentioning

confidence: 99%

Altered swelling and ion fluxes in articular cartilage as a biomarker in osteoarthritis and joint immobilization: a computational analysis

Manzano

Doblaré

et al. 2015

J. R. Soc. Interface.

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“…Several studies on animals of early OA Figure 5 Micro-CT 3D models of a representative sample of each experimental group. Cartilage swelling characteristic of the first stages of OA can be clearly appreciated in OA samples, whereas diacerein-treated samples recover normal cartilage morphology like CTRL with magnetic resonance imaging and histomorphometry identified an initial phase of cartilage hypertrophy prior to its degeneration and loss [35,36]. The explanation has not been found yet, and it is not known whether it is the tissue expression to an inflammatory and repair phenomenon or if it represents a permanent damage of the joint tissue.…”

Section: Discussionmentioning

confidence: 96%

Effects of diacerein on cartilage and subchondral bone in early stages of osteoarthritis in a rabbit model

Permuy

López

Miño

et al. 2012

Bone

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Background: Osteoarthritis is thought to be the most prevalent chronic and disabling joint disease in animals and humans. At present, there is no ideal treatment option. The aim of this study was to assess the effects of the treatment with oral diacerein on articular cartilage, synovial membrane and subchondral bone in an experimental rabbit model of osteoarthritis by micro-CT evaluation and histological analysis. To this purpose, osteoarthritis was surgically induced on one knee of 16 rabbits using the contralateral knee as healthy controls. Treatment was started three weeks later and lasted eight weeks. Animals were divided into two groups for treatment: Placebo (treated daily with oral saline) and diacerein (treated orally with 1.5 mg/kg/day of diacerein).

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“…A RM destaca-se por ser uma excelente técnica para avaliar alterações osteocondrais, porém, algumas anormalidades morfológicas da cartilagem só podem ser visibilizadas em lesões bem estabelecidas (Calvo et al 2004). De fato, isto constituiu uma característica importante da RM na atual avaliação experimental, pois alguns detalhes a respeito da regularidade e do preenchimento da superfície articular diferiram das avaliações macro e microscó-picas.…”

Section: Discussionunclassified

Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

et al. 2017

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ABSTRACT. - This study aimed to determine the characteristics and applicability of magnetic resonance imaging (MRI) in the evaluation of autogenous osteochondral graft in intact or macerated format, with or without insulin-like growth factor type 1 (IGF-1) used in repair of cartilage lesions induced in rabbits. Nine New Zealand rabbits were used, in which 18 stifle joints underwent grafting procedure in the femoral trochlear groove. These were divided into four groups, referred as intact osteochondral graft + IGF-1 (n=5), intact osteochondral graft + saline solution (n=4), macerated osteochondral graft + IGF-1 (n=5) and macerated osteochondral graft + saline solution (n=4). Animals were euthanized 12 weeks after surgery and the joints were subjected to MRI using a high magnetic field scanner of 1.5 Tesla. In addition, samples of grafting sites were subjected to anatomopathological examination. The MRI was effective as a noninvasive method to evaluate the repair tissue in osteochondral grafts in articular cartilage of the femur of rabbits by providing complementary data to macroscopic and histological examinations. Through these images and anatomopathological examinations satisfactory results were observed in relation to the repair process of autogenous osteochondral grafts in cartilage of rabbits, regardless of its format or the addition of IGF-1. RESUMO.-Este trabalho teve o objetivo de determinar as características e a aplicabilidade do exame de ressonância magnética na avaliação de enxerto ostecondral autógeno, em formato íntegro ou macerado, associado ou não ao fator de crescimento semelhante à insulina tipo 1 (IGF-1), utilizado no reparo de lesões induzidas na cartilagem articular de coelhos. Foram utilizados 9 coelhos da linhagem Nova Zelândia, em que as 18 articulações fêmoro-tíbio-patelares foram submetidas à enxertia osteocondral autógena no sulco troclear femoral. Estas foram divididas em quatro grupos, denominados como enxerto osteocondral íntegro + IGF-1 (n=5), enxerto osteocondral íntegro + solução fisioló-gica (n=4), enxerto osteocondral macerado + IGF-1 (n=5) e enxerto osteocondral macerado + solução fisiológica (n=4). Os animais foram eutanasiados em 12 semanas após a cirurgia e as articulações foram submetidas ao exame de ressonância magnética utilizando um aparelho scanner de 1,5 Tesla de alto campo magnético. Além disso, amostras dos locais de enxertia foram submetidas aos exames anatomopatológicos. O exame de ressonância magnética mostrou--se eficaz como um método não invasivo para avaliação do tecido de reparação em enxertos osteocondrais na cartilagem articular do fêmur de coelhos, fornecendo dados complementares aos exames macroscópicos e histológicos. Por

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Histopathological correlation of cartilage swelling detected by magnetic resonance imaging in early experimental osteoarthritis

Cited by 128 publications

References 31 publications

Altered swelling and ion fluxes in articular cartilage as a biomarker in osteoarthritis and joint immobilization: a computational analysis

Altered swelling and ion fluxes in articular cartilage as a biomarker in osteoarthritis and joint immobilization: a computational analysis

Effects of diacerein on cartilage and subchondral bone in early stages of osteoarthritis in a rabbit model

Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

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