Histopathological Classification of Lesions associated with Natural Paratuberculosis Infection in Cattle

González, J.; Geijó, Mariví; García-Pariente, Carlos; Verna, Andrea Elizabeth; Corpa, J. M.; Reyes, Luís E.; Ferreras, M.C.; Juste, Ramón A.; Marı́n, Juan Francisco Garcı́a; Pérez, Valentín Pérez

doi:10.1016/j.jcpa.2005.04.007

Cited by 131 publications

(227 citation statements)

References 23 publications

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“…First of all, clinical signs and lesions observed in deer with the multibacillary form of the disease match the lesion descriptions for cattle and small ruminants in multibacillary cases (Buergelt et al 1978, Clarke 1997, Huda and Jensen 2003, Gonzalez et al 2005, Mackintosh et al 2007. However, lesions in the large intestine with diffuse inflammatory infiltration and AFB was observed as well as multifocal granulomas in liver, probably due to more susceptibility of the deer or may be a more pathogenic Map strain.…”

Section: Discussionsupporting

confidence: 64%

“…Paratuberculosis diagnosis in deer is based on Map detection in faeces, postmortem and histopathological examination of tissues (specially the lower part of the jejunum, ileum, ileocecal valve and its associated lymph nodes) (Clarke 1997). Histopathological assessment includes different grading systems and disease classification, such as paucibacillary and multibacillary, already described for several species with paratuberculosis (Carrigan and Seaman 1990, Clarke and Little 1996, Perez et al 1996, Gonzalez et al 2005, Balseiro et al 2008, Clark et al 2010. However, the histopathological observation of lesions is insufficient to confirm the disease, especially due to the similarity of lesions caused by Map and M. avium subsp.…”

Section: Introductionmentioning

confidence: 99%

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Paratuberculosis or avian tuberculosis in red deer with chronic diarrhea?

et al. 2014

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SUMMARYMycobacterium avium subsp. paratuberculosis (Map) is the etiological agent of paratuberculosis. In Chile, information about Map isolation from both domestic ruminant and wildlife species has been accumulating, but it has extended to other species. In Chile, deer farming activity has been focused in hunting and meat production. No paratuberculosis surveillance has been reported for these source herds. In the present study, chronic diarrhea and poor body condition in some animals belonging to a deer farm was informed. Four adult affected hinds were euthanized under suspicion of paratuberculosis. In all animals macroscopic and microscopic lesions consistent with paratuberculosis were observed. Map-positive culture results from faecal and tissue samples, complemented with positive-PCR-results from fixed tissues are informed. Evidence has been found on cattle and deer populations being connected since molecular characterization of the Map isolated from deer lack of variation between the cattle control strain. The latter could indicate that both species share the same bacteria, suggesting there is interspecies transmission. Complementary diagnostic methods were accurate to diagnose paratuberculosis and to differentiate the clinical deer case from other mycobacterial infection. The epidemiological findings suggest that the infection in the deer farm could be transmitted from a cattle herd, making this the first reported case of paratuberculosis in deer farm in Chile.Key words: deer, histopathology, paratuberculosis, PCR. RESUMENMycobacterium avium subsp. paratuberculosis (Map) es el agente etiológico de la patuberculosis. En Chile, antecedentes del aislamiento de Map tanto en rumiantes domésticos como en especies de vida silvestre se han ido acumulando a través del tiempo, sin embargo, esto tiene que estar afectando también a otras especies. En Chile, la crianza de ciervos se ha focalizado en dos objetivos: cotos de caza y producción de carnes exóticas, no existiendo un programa de vigilancia ni siendo reportada esta enfermedad en rebaños de este tipo. Este trabajo reporta la presencia de diarrea crónica y pobre condición corporal en algunos animales pertenecientes a una granja de ciervos donde se sospechó la presencia de paratuberculosis. Cuatro ciervos adultos fueron eutanaziados bajo esta sospecha. Todos los animales presentaron lesiones macro y microscópicas compatibles con paratuberculosis. Resultados positivos al cultivo bacteriológico a partir de heces y muestras de tejido, además de PCR a partir de tejido fijado evidencian la infección. De acuerdo a los resultados de caracterización molecular de las cepas aisladas de los ciervos que no muestran variación entre cepas control de bovino, se evidencia que ambas poblaciones estarían conectadas. Complementariamente, los métodos diagnósticos utilizados fueron muy precisos para diagnosticar paratuberculosis y para diferenciar casos clínicos en ciervos de otras micobacteriosis. Las características epidemiológicas descritas en este estudio sugieren que la infecció...

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Section: Discussionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Paratuberculosis or avian tuberculosis in red deer with chronic diarrhea?

et al. 2014

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“…In ovine paratuberculosis, Pe´rez et al (1996) described small ''tuberculoid'' granulomas in the ileocaecal lymphoid tissue (Peyer's patches). This type of lesion, also referred as a ''focal lesion'', was later reported in goats (Corpa et al, 2000) and cattle (Gonza´lez et al, 2005). A ''multibacillary'' form, in which macrophages were filled with numerous mycobacteria, was also described (Carrigan and Seaman, 1990; Pe´rez et al, 1996; Clarke, 1997;Corpa et al, 2000;Gonza´lez et al, 2005).…”

Section: Introductionmentioning

confidence: 92%

“…Paratuberculosis is well studied in domestic ruminants, but there are relatively few reports in free-ranging deer (Jessup and Williams, 1999;Pavlik et al, 2000;Alvarez et al, 2005), infection in such animals having been first diagnosed in five free-ranging fallow deer (Dama dama) in Spain in 1997 (Marco et al, 2002). Classification of lesions has been described in sheep (Stamp and Watt, 1954;Carrigan and Seaman, 1990;Pe´rez et al, 1996), goats (Paliwal et al, 1985;Corpa et al, 2000) and cattle (Buergelt et al, 1978;Gonza´lez et al, 2005). In ovine paratuberculosis, Pe´rez et al (1996) described small ''tuberculoid'' granulomas in the ileocaecal lymphoid tissue (Peyer's patches).…”

Section: Introductionmentioning

confidence: 99%

Histopathological Classification of Lesions Observed in Natural Cases of Paratuberculosis in Free-ranging Fallow Deer (Dama dama)

Balseiro

Marı́n

Solano

et al. 2008

Journal of Comparative Pathology

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SummaryNinety-five adult fallow deer, legally hunted in the Regional Hunting Reserve of El Sueve (Northern Spain), were subjected to a post-mortem examination for paratuberculosis, samples being taken from the proximal and distal jejunum, proximal and distal ileum, ileocaecal valve and associated lymph nodes. The lesions were divided into four categories. Focal lesions (n ¼ 19 cases) consisted of small granulomas, mainly in the jejunal and ileal lymph nodes. Multifocal lesions (n ¼ 4) consisted of well-demarcated granulomas in the intestinal lymphoid tissue and also in the intestinal lamina propria. Diffuse multibacillary lesions (n ¼ 2) were characterized by a severe granulomatous enteritis and lymphadenitis. Macrophages and numerous Langhans giant cells containing many mycobacteria were present, resulting in macroscopical changes in the normal gut morphology. These changes were found from the proximal jejunum to the ileocaecal valve, but lesions were always particularly severe in the distal jejunum. In diffuse intermediate (multibacillaryelymphocytic) lesions (n ¼ 3) the infiltrate consisted of lymphocytes, macrophages and Langhans giant cells, with small numbers of mycobacteria. Mycobacterium avium subspecies paratuberculosis was identified by a polymerase chain reaction technique. The widespread occurrence of paratuberculosis in fallow deer in this Reserve represents a potential source of infection for other susceptible species.

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“…The relative number of AFB is given the lowest weight in this system since both paucibacillary and pleuribacillary forms of JD are recognized in some species affected. Alternative lesion grading systems based on classification of lesions of natural cases of paratuberculosis in sheep (Perez et al, 1996), goat (Corpa et al, 2000) and cattle (Gonzalez et al, 2005), granuloma counting for sheep (Juste et al, 1994), and for deer have been proposed. on these ovine strains, it is critical that each isolate used be genotyped to allow future comparison.…”

Section: Long-term Ovine Map Challenge Modelmentioning

confidence: 99%

Experimental challenge models for Johne's disease: A review and proposed international guidelines

Hines

Stabel

Sweeney

et al. 2007

Veterinary Microbiology

120

140

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An international committee of Johne's disease (JD) researchers was convened to develop guidelines for JD challenge studies in multiple animal species. The intent was to develop and propose international standard guidelines for models based on animal species that would gain acceptance worldwide. Parameters essential for the development of long-term and short-term infection models were outlined and harmonized to provide a ''best fit'' JD challenge model for cattle, goats, sheep, cervids, and mice. These models will be useful to study host-pathogen interactions, host immunity at the local and systemic level, and for evaluating vaccine candidates and therapeutics. The consensus guidelines herein list by animal species strains of Mycobacterium avium subsp. paratuberculosis used, challenge dose, dose frequency, age of challenge, route of challenge, preparation of inoculum, experimental animal selection, quality control, minimal experimental endpoints and other parameters. #

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Histopathological Classification of Lesions associated with Natural Paratuberculosis Infection in Cattle

Cited by 131 publications

References 23 publications

Paratuberculosis or avian tuberculosis in red deer with chronic diarrhea?

Paratuberculosis or avian tuberculosis in red deer with chronic diarrhea?

Histopathological Classification of Lesions Observed in Natural Cases of Paratuberculosis in Free-ranging Fallow Deer (Dama dama)

Experimental challenge models for Johne's disease: A review and proposed international guidelines

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