Histopathological Characterization of Cutaneous Delayed-type Hypersensitivity and Correlations with Intestinal Pathology and Systemic Immune Responses in Sheep with Paratuberculosis

Plain, Karren M.; Begg, Douglas J.; Whittington, Richard J.

doi:10.1016/j.jcpa.2015.05.005

Cited by 8 publications

(4 citation statements)

References 37 publications

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“…The pathogenesis of MAP infection in ruminants and other species shows typical Th1/Th2 type of immune profile [ 34 – 37 ]. Recently, a study showed that the histopathology of the cutaneous delayed type hypersensitivity response were almost similar as the stage of intestinal pathology of MAP infection [ 38 ]. Moreover, this also matched well with the systemic humoral and cellular immunity.…”

Section: Discussionmentioning

confidence: 99%

Systems Analysis of Early Host Gene Expression Provides Clues for Transient Mycobacterium avium ssp avium vs. Persistent Mycobacterium avium ssp paratuberculosis Intestinal Infections

et al. 2016

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It has long been a quest in ruminants to understand how two very similar mycobacterial species, Mycobacterium avium ssp. paratuberculosis (MAP) and Mycobacterium avium ssp. avium (MAA) lead to either a chronic persistent infection or a rapid-transient infection, respectively. Here, we hypothesized that when the host immune response is activated by MAP or MAA, the outcome of the infection depends on the early activation of signaling molecules and host temporal gene expression. To test our hypothesis, ligated jejuno-ileal loops including Peyer’s patches in neonatal calves were inoculated with PBS, MAP, or MAA. A temporal analysis of the host transcriptome profile was conducted at several times post-infection (0.5, 1, 2, 4, 8 and 12 hours). When comparing the transcriptional responses of calves infected with the MAA versus MAP, discordant patterns of mucosal expression were clearly evident, and the numbers of unique transcripts altered were moderately less for MAA-infected tissue than were mucosal tissues infected with the MAP. To interpret these complex data, changes in the gene expression were further analyzed by dynamic Bayesian analysis. Bayesian network modeling identified mechanistic genes, gene-to-gene relationships, pathways and Gene Ontologies (GO) biological processes that are involved in specific cell activation during infection. MAP and MAA had significant different pathway perturbation at 0.5 and 12 hours post inoculation. Inverse processes were observed between MAP and MAA response for epithelial cell proliferation, negative regulation of chemotaxis, cell-cell adhesion mediated by integrin and regulation of cytokine-mediated signaling. MAP inoculated tissue had significantly lower expression of phagocytosis receptors such as mannose receptor and complement receptors. This study reveals that perturbation of genes and cellular pathways during MAP infection resulted in host evasion by mucosal membrane barrier weakening to access entry in the ileum, inhibition of Ca signaling associated with decreased phagosome-lysosome fusion as well as phagocytosis inhibition, bias toward Th2 cell immune response accompanied by cell recruitment, cell proliferation and cell differentiation; leading to persistent infection. Contrarily, MAA infection was related to cellular responses associated with activation of molecular pathways that release chemicals and cytokines involved with containment of infection and a strong bias toward Th1 immune response, resulting in a transient infection.

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Section: Discussionmentioning

confidence: 99%

Systems Analysis of Early Host Gene Expression Provides Clues for Transient Mycobacterium avium ssp avium vs. Persistent Mycobacterium avium ssp paratuberculosis Intestinal Infections

et al. 2016

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“…Sin embargo, a medida que avanza la enfermedad, la HTR disminuye y puede estar ausente cuando se presenta sintomatología. Posteriormente, los anticuerpos detectan la HTR y generalmente aumenta a medida que empeoran las lesiones en el animal (Gulliver et al, 2015).…”

Section: Patogenia Y Transmisiónunclassified

Paratuberculosis bovina, revisión de literatura

et al. 2021

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La paratuberculosis bovina (PTB) es una enfermedad crónica causada por el bacilo Mycobacterium avium subespecie paratuberculosis (MAP). Tiene distribución a nivel mundial y genera pérdidas económicas para los productores por el bajo rendimiento productivo de los animales y el amplio periodo de incubación, en el cual es excretada a través de la materia fecal sin signos clínicos. Así mismo, se reporta que los animales más susceptibles de contraer la enfermedad son los terneros. Entre los métodos de diagnóstico utilizados para la MAP, están PCR, ELISA, cultivo bacteriano de materia fecal, el examen para detección de MAP en muestras de tejido y la prueba de hipersensibilidad cutánea retardada Johnina intradérmica. La MAP es una bacteria con posible potencial zoonótico por su relación con la enfermedad de Crohn. Debido a que no hay tratamiento para la PTB, se deben intensificar los mecanismos de control en las producciones.

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“…Those with subclinical disease and thus no clinical symptoms can remain undetected for years during which time, as carriers of MAP, they can shed the infectious agent into the external environment [5, 6]. Successful control of paratuberculosis has been difficult because commonly used diagnostic tests are not accurate until the later stage of infection [7, 8].…”

Section: Introductionmentioning

confidence: 99%

Magnetic Separation Methods for the Detection ofMycobacterium aviumsubsp.paratuberculosisin Various Types of Matrices: A Review

Husakova

Dziedzinská

Slaná

2017

BioMed Research International

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The main reasons to improve the detection of Mycobacterium avium subsp. paratuberculosis (MAP) are animal health and monitoring of MAP entering the food chain via meat, milk, and/or dairy products. Different approaches can be used for the detection of MAP, but the use of magnetic separation especially in conjunction with PCR as an end-point detection method has risen in past years. However, the extraction of DNA which is a crucial step prior to PCR detection can be complicated due to the presence of inhibitory substances. Magnetic separation methods involving either antibodies or peptides represent a powerful tool for selective separation of target bacteria from other nontarget microorganisms and inhibitory sample components. These methods enable the concentration of pathogens present in the initial matrix into smaller volume and facilitate the isolation of sufficient quantities of pure DNA. The purpose of this review was to summarize the methods based on the magnetic separation approach that are currently available for the detection of MAP in a broad range of matrices.

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Histopathological Characterization of Cutaneous Delayed-type Hypersensitivity and Correlations with Intestinal Pathology and Systemic Immune Responses in Sheep with Paratuberculosis

Cited by 8 publications

References 37 publications

Systems Analysis of Early Host Gene Expression Provides Clues for Transient Mycobacterium avium ssp avium vs. Persistent Mycobacterium avium ssp paratuberculosis Intestinal Infections

Systems Analysis of Early Host Gene Expression Provides Clues for Transient Mycobacterium avium ssp avium vs. Persistent Mycobacterium avium ssp paratuberculosis Intestinal Infections

Paratuberculosis bovina, revisión de literatura

Magnetic Separation Methods for the Detection ofMycobacterium aviumsubsp.paratuberculosisin Various Types of Matrices: A Review

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