Histopathological changes in tumor budding between biopsy and resected specimens from patients treated with preoperative S‐1 chemotherapy for oral cancer
Abstract:Background
S‐1 is an oral anticancer agent containing tegafur, a prodrug of 5‐fluorouracil (5‐FU). Preoperative S‐1 chemotherapy is performed to prevent tumor proliferation before surgery in oral squamous cell carcinoma (OSCC). The aim of this study was to evaluate the effects of preoperative S‐1 chemotherapy on tumor budding.
Methods
We examined 248 cases of OSCC and evaluated the budding scores in biopsy and resected specimens by keratin immunohistochemistry.
Results
In S‐1‐untreated patients, the budding sc… Show more
“…All six papers included were retrospective, and were divided into two groups based on the aim of the study (Tab. II) 48,49,[58][59][60][61] :…”
Section: Resultsmentioning
confidence: 99%
“…Comparison of tissue markers on biopsy and post-operative samples. Three papers [58][59][60] compared the values of biomarkers measured on patient's biopsy and post-operative samples from the same patients in order to evaluate their concordance and reliability. Almangush et al 58 reviewed the slides of 100 patients (81 = Stage I-II) with SCC of the mobile tongue treated with surgical excision.…”
Section: Resultsmentioning
confidence: 99%
“…III) and LVI, and found that the depth of infiltration measured on biopsies was lower than that on post-operative samples, while there was a good correlation of budding score in pre-treatment and post-treatment samples. Seki Soda et al 60 recently analysed the relationship between TBS in biopsies and in resected specimens of 248 patients with OSCC and the effect of pre-operative chemotherapy on TBS. The authors did not report staging, but pointed out that 141 of 248 patients received preoperative chemotherapy.…”
“…All six papers included were retrospective, and were divided into two groups based on the aim of the study (Tab. II) 48,49,[58][59][60][61] :…”
Section: Resultsmentioning
confidence: 99%
“…Comparison of tissue markers on biopsy and post-operative samples. Three papers [58][59][60] compared the values of biomarkers measured on patient's biopsy and post-operative samples from the same patients in order to evaluate their concordance and reliability. Almangush et al 58 reviewed the slides of 100 patients (81 = Stage I-II) with SCC of the mobile tongue treated with surgical excision.…”
Section: Resultsmentioning
confidence: 99%
“…III) and LVI, and found that the depth of infiltration measured on biopsies was lower than that on post-operative samples, while there was a good correlation of budding score in pre-treatment and post-treatment samples. Seki Soda et al 60 recently analysed the relationship between TBS in biopsies and in resected specimens of 248 patients with OSCC and the effect of pre-operative chemotherapy on TBS. The authors did not report staging, but pointed out that 141 of 248 patients received preoperative chemotherapy.…”
Tumor Budding (TB) represents a single cancer cell or a small cluster of less than five cancer cells on the infiltrative tumor front. Accumulating evidence suggests TB is an independent prognostic factor in oral squamous cell carcinoma (OSCC). However, its exact role is not yet elucidated, and a standardized scoring system is still necessary. The study aims to extensively review the literature data regarding the prognostic role of TB in OSCC. The results of TB are an independent prognostic factor of poor survival outcomes in OSCC. To date, the manual detection of hematoxylin and eosin-staining or pancytokeratin-immunostaining sections are the most commonly used methods. Between the several cut-offs, the two-tier system with five buds/field cut-offs provides better risk stratification. The prognostic role of the BD model in predicting survival outcomes was extensively validated; however, the inclusion of DOI, which is already a staging parameter, encouraged other authors to propose other models, integrating TB count with other adverse risk factors, such as the tumor–stroma ratio and tumor-infiltrated lymphocytes. The prognostic relevance of TB in OSCC highlights its evaluation in daily pathological practice. Therefore, the TB detection method and the TB scoring system should be validated based on tumor stage and site.
BackgroundIt has been reported in oral squamous cell carcinoma (OSCC) that myeloid‐derived suppressor cells infiltrate tumor tissues. This study examined whether S‐1 chemotherapy changes immune cell populations in the tumor microenvironment.MethodsWe examined 71 patients with of OSCC, including 51 patients who received preoperative S‐1 chemotherapy. Immunohistochemistry for PD‐L1, CD8, forkhead box protein 3 (FOXP3), and CD15 was performed using biopsy and resected specimens.ResultsThe numbers of CD8+, FOXP3+, and CD15+ cells in resected specimens were significantly decreased by S‐1 chemotherapy. The reduction of the proportion of CD15+ cells significantly differed between responders and nonresponders. Most responders were distributed into the group with low PD‐L1 expression and a low density of CD8+ cells before chemotherapy. Furthermore, many patients with recurrence exhibited a high density of CD15+ cells in biopsy specimens.ConclusionPreoperative S‐1 chemotherapy can potentially improve prognosis by reducing CD15+ cells in the tumor microenvironment.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.