Histopathological and ultrastructural indices for the assessment of glyphosate-based herbicide cytotoxicity in decapod crustacean hepatopancreas

Melo, Madson Silveira de; Santos, Thiciane Patrycia Gonçalves dos; Jaramillo, Michael L.; Nezzi, Luciane; Müller, Yara Maria Rauh; Nazari, Evelise Maria

doi:10.1016/j.aquatox.2019.03.007

Cited by 41 publications

(6 citation statements)

References 40 publications

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“…The tissue structure of fresh crayfish was determined using a previously reported method, with slight modification. 19,20 Crayfish tissues were fixed with 4% paraformaldehyde for over 24 h. The tissue samples were trimmed with a scalpel and dehydrated with different concentrations of alcohol (75% for 4 h, 85% for 2 h, 90% for 2 h, 95% for 1 h and 100% for 30 min). Tissue samples were treated with transparent xylene, embedded in paraffin and cut into sections with a thickness of 4 μm.…”

Section: Microstructure Assaymentioning

confidence: 99%

Effects of high hydrostatic pressure on peelability and quality of crayfish(Procambarus clarkii)

Shen

Qiu

et al. 2023

J Sci Food Agric

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BACKGROUNDPeeling of crayfish is a very important process in production. Crayfish peeling by machine can increase production efficiency and enhance the safety in production process. The tight muscle‐shell attachment causes difficulty in peeling freshly caught crayfish. However, few studies have explored the changes in crayfish quality under favourable shell‐loosening treatments.RESULTSIn this study, the shell‐loosening properties of crayfish and changes in crayfish quality, microstructure, and protein fluorescent features were investigated after high hydrostatic pressure treatment (HHP). New methods were established to measure the peeling performance of crayfish, which are peelabibity and meat yelid rate (MYR). The normalization of peelability and MYR were verified by different weights of crayfish tails and different treatments. The peeling effect of HHP‐treated crayfish was evaluated by a new quantitative measurement method, and meat yield rate (MYR) was calculated. The results showed that all the HHP treatments reduced crayfish peeling work and increased MYR. The HHP treatment provided better crayfish quality in terms of texture and color and enlarged the shell‐loosening gap. Among all HHP treatments, 200 MPa treatment exhibited a lower peeling work , higher MYR, and an expansion of the shell‐loosening gap reaching up to 573.8 um. At the same time, 200MPa treatment can maintain the quality of the crayfish.CONCLUSIONThe findings outlined above suggest that high pressure is a promising method used to loosen crayfish shells. 200 MPa is an optimal HHP treatment condition for crayfish peeling, exhibiting a promising application in industrial processing.This article is protected by copyright. All rights reserved.

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Section: Microstructure Assaymentioning

confidence: 99%

Effects of high hydrostatic pressure on peelability and quality of crayfish(Procambarus clarkii)

Shen

Qiu

et al. 2023

J Sci Food Agric

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“…De Melo et al. (2019) evaluated cellular responses of the hepatopancreas detoxification process in invertebrates. Prawn Macrobrachium potiuna were exposed as model invertebrate to various concentration levels of glyphosate‐based herbicides.…”

Section: Toxicologymentioning

confidence: 99%

Pesticides and herbicides

Choudri

Charabi

Al‐Nasiri

et al. 2020

Water Environment Research

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This paper highlights a review of scientific papers published in the year 2019 regarding pesticides and herbicides. The scientific review presented in this paper includes the presence and occurrence of pesticides and herbicides in the environment. The entire review divided into different sections, which are grouped into four main sections. Each of these sections provides studies conducted on toxicology, ecological risk assessment, strategies of treatment, policies, modeling, and guidelines regarding pesticides and herbicides management.

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“…The active ingredient of these herbicides, the glyphosate molecule (N‐(phosphomethyl)glycine), inhibits the enzyme 5‐enolpyruvyl‐3‐phosphate synthase, which is present in the shikimate pathway and is responsible for synthesizing essential aromatic amino acids for plant development 6,7 . Although this enzyme is not found in animals, several studies demonstrate GBH's disruptive potential in non target organisms such as crustaceans (decrease in reproduction), 8,9 annelids (increase of enzyme superoxide dismutase), 10 fish (increase in mortality, decrease in cell viability, inhibited mitochondrial complex enzymatic activity, and ultrastructural changes in the ovarian follicular), 11–13 amphibians (morphogenesis abnormalities, micronuclei formation, and erythrocyte nuclear abnormalities), 14,15 rats (decrease in total counting sperm and the weight of seminal vesicle), 16,17 and humans (sperm motility) 18,19 …”

Section: Introductionmentioning

confidence: 99%

Impact of exposure to glyphosate‐based herbicide on morphological and physiological parameters in embryonic and larval development of zebrafish

Durante,

Hollmann,

Nazari

2023

Environmental Toxicology

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Glyphosate‐based herbicides (GBH) have been commonly used in agriculture to inhibit weed growth and increase yields. However, due to the high solubility of these herbicides in water, they can reach aquatic environments, by infiltration, erosion, and/or lixiviation, affecting non target organisms. Thus, this study aimed to characterize the toxicity of GBH Roundup WG® (RWG®) during the embryonic and larval development of Danio rerio. Embryos (3 hours post fertilization, hpf–until hatching) and larvae (3 days post fertilization, dpf to 6 dpf) were exposed to concentrations of 0.065 and 6.5 mg L−1. They were evaluated for survival, hatching, spontaneous movements, heartbeat, morphology, and morphometry by in vivo photographs in microscope, cell proliferation and apoptosis by immunohistochemistry, and exploratory behavior and phototropism by video recording. Our results showed an increase in embryo and larvae mortality in those exposed to 0.065 mg L−1, as well as a reduction in spontaneous embryo movements. The larval heartbeats showed a decrease at 4 dpf in the group exposed to 0.065 mg L−1 and an increase at 5 and 6 dpf in both exposed groups. Cell proliferation was reduced in both groups exposed in embryos and only in the 0.065 mg L−1 group in larvae, while cell death increased in embryos exposed to 6.5 mg L−1. These results demonstrated the toxic effect of low concentrations of the herbicide RWG® during embryonic and larval development of non target organisms, as well as the importance of constantly reviewing acceptable limits for exposure in natural environments.

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Histopathological and ultrastructural indices for the assessment of glyphosate-based herbicide cytotoxicity in decapod crustacean hepatopancreas

Cited by 41 publications

References 40 publications

Effects of high hydrostatic pressure on peelability and quality of crayfish(Procambarus clarkii)

Effects of high hydrostatic pressure on peelability and quality of crayfish(Procambarus clarkii)

Pesticides and herbicides

Impact of exposure to glyphosate‐based herbicide on morphological and physiological parameters in embryonic and larval development of zebrafish

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