Histopathological and Serological Diagnosis of Avian Reticuloendotheliosis in Cross-bred Chicken Farms in Delta Egypt

El-Abasy, Moshira; El-Gohary, Abd El-Galiel A.; El-Sawy, Asia; Hafez, Hafez M.; El-Adawy, Hosny

doi:10.3923/ajava.2016.272.279

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…The positive percentage inside the farm flocks ranged from 40-100%, GMT ranged from (392-15524) and CV ranged from (14-156), indicating different individuals infected by REV, which may be related to the REV incidence in different regions or susceptibility to REV of different breeds flocks. The serology results were agreed with Moshira et al 2016, where the serological prevalence for the REV antibody ranged from 25-100 % at 12 th and 25 th weeks of age for crossbreed chicken farms, respectively. Zhao et al 2012, where Serum samples analysis revealed 32.16% samples positive for REV-antibody.…”

Section: Discussionsupporting

confidence: 73%

Highlight on Reticuloendotheliosis virus (REV) in commercial chicken flocks

2023

Egyptian Journal of Animal Health

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I n this study, the reticuloendotheliosis (REV) infection status was monitored through antibody determination and molecular detection in chickens in different provinces of Egypt. We investigated the occurrence and genetic characteristics of REVs in chickens' flocks from January 2019 to March 2021 as a PCR test examined 39 commercial chicken farms (27 layers and 12 broiler breeders) from 11 provinces of Egypt. Results showed six farms (15.4%) were REV-positive in 3 provinces (AL Sharqia, Al Menya, and Al Behera). Four REV-positive farms were genetically sequenced. 24 of these 39 farms (18 layers and 6 broiler breeders) were examined by ELISA test for antibodies detection against REV and ALV (subtype A and B). 22 farms (91.7%) were REV antibodies positive in all 11 provinces test-

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Section: Discussionsupporting

confidence: 73%

Highlight on Reticuloendotheliosis virus (REV) in commercial chicken flocks

2023

Egyptian Journal of Animal Health

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“…The primers used for this purpose were (5´-CATACCTGCAGCCAATGGTT-3´) and (3´ AATGTTGTACCGAAGTACT-5´). The Genbank accession no followed was KX278301 8 . PCR reaction was performed in a 50μl reaction mixture, containing 5µL PCR buffer, 3µL MgCl 2 , 1µL dNTPs, each primer 1µL, Taq DNA polymerase (Fermentas) 1µL and 1µL template DNA PCR conditions in the thermal cycler (Applied Biosystems 2720) were: initial denaturation at 95°C for 5 minutes; 32 cycles of denaturation at 95°C for 30 seconds; annealing at 55°C for 1 minute; primary extension at 72°C for 1 minute; final extension at 72°C for 6 minutes and final hold at 4°C.After completion of polymerase chain reaction the samples were loaded on the gel.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…Each well was loaded with 7.5 µL of sample alongside 3-5 µL of 100bp DNA ladder or marker. Gel was run at 120 volts for 40 minutes in gel electrophoresis instrument and then visualized by UV transilluminator 8 .…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Serological Investigation and Molecular Characterization of Reticuloendotheliosis Virus from Breeder Flocks in Pakistan by Polymerase Chain Reaction

2018

J LIAQUAT UNI MED HEALTH SCI

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Serological and molecular identification of Reticuloendotheliosis virus (REV) in chickens in Sudan

Alfaki

Hussien

Elsheikh

et al. 2019

Veterinary Medicine & Sci

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BackgroundReticuloendotheliosis virus (REV) is a gammaretrovirus that belongs to the family of Retroviridae. The infection can result in immunosuppression, runting syndrome, high mortality, acute reticular cell neoplasia or T‐ and/ or B‐cell lymphoma, in a variety of domestic and wild birds. The disease is widespread around the world. No related data have been reported in Sudan about the disease. The present study was conducted to determine the prevalence of REV antibodies and DNA in local and commercial breeds of chickens older than 20 weeks from June 2014 to February, 2017.MethodsA total of 460 sera samples and 150 (50 liver and 100 spleen) tissue samples were collected from local and commercial breeds of chickens older than 20 weeks and screened for anti‐REV antibodies in four states of Sudan using a commercial REV antibody ELISA test kit (IDEXX). Polymerase chain reaction (PCR) was performed to detect REV DNA in tissue samples in Khartoum State.ResultsThe results revealed that the overall seroprevalence of REV was 74.6% among local and commercial chicken breeds, but in commercial it was 79.5% (190/239) and 69.2% in local breeds (153/221). One hundred and fifty tissue samples of chickens (50 liver, 100 spleen) were tested using PCR for detection of REV using primer sets of the conserved region in envelope glycoprotein (env) gene with a band length of 850 bp. Five out of 50 (10%) liver samples were RE provirus DNA positive detected by PCR, whereas 15 out of 100 (15%) spleen samples were PCR positive. Univariate analysis revealed there was a difference (p ≤ 0.05) between locality and breed of chickens and seropositivity to REV.ConclusionsThe prevalence of the disease was high in Sudan and more studies are needed to evaluate the epidemiology and pathogenesis of the virus.

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Histopathological and Serological Diagnosis of Avian Reticuloendotheliosis in Cross-bred Chicken Farms in Delta Egypt

Cited by 3 publications

References 15 publications

Highlight on Reticuloendotheliosis virus (REV) in commercial chicken flocks

Highlight on Reticuloendotheliosis virus (REV) in commercial chicken flocks

Serological Investigation and Molecular Characterization of Reticuloendotheliosis Virus from Breeder Flocks in Pakistan by Polymerase Chain Reaction

Serological and molecular identification of Reticuloendotheliosis virus (REV) in chickens in Sudan

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