Histopathological and molecular evaluation of Holstein-Friesian cows postpartum: Toward an improved understanding of uterine innate immunity

Chapwanya, Aspinas; Meade, Kieran G.; Doherty, Michael L.; Callanan, John J.; Mee, John F.; O’Farrelly, Cliona

doi:10.1016/j.theriogenology.2009.01.006

Cited by 132 publications

(105 citation statements)

References 43 publications

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“…Bacterial colonization of the uterine lumen produces immune response stimulation. Polymorphonuclear cells inflame the endometrium, installing subclinical endometritis without systemic signs that delay uterine involution (7).…”

Section: Introductionmentioning

confidence: 99%

Endometrial cytology as a diagnostic tool for subclinical endometritis in beef heifers

Moscuzza

Alvarez²,

Gutierrez³

et al. 2015

Turk J Vet Anim Sci

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The obstetric assistance of dystocia in field conditions promotes greater bacterial contamination of the uterus, causing subclinical endometritis. The aim of this study was to determine the occurrence of subclinical endometritis in beef heifers receiving professional calving assistance, and to evaluate endometrial cytology as a diagnostic technique compared to uterine biopsy. A group of 829 Angus heifers were assisted at calving. Dystocia was classified based on difficulty at assistance, which was estimated as complex or simple. At the end of the calving season, 93 dystocic heifers were reported (10.7%). The percentage of simple resolution and complex resolution was 88.2% and 11.8%, respectively. After 60 days of calving, all animals underwent a gynecological examination, endometrial cytology, and uterine biopsy. In all, 38.9% of heifers presented subclinical endometritis diagnosed by endometrial cytology (sensitivity = 78.6%, specificity = 95.8%). There was a significant association (r = 0.8528) between uterine cytology and uterine biopsy. No associations were observed between endometrial cytology or biopsy and calf viability, or between endometrial cytology or biopsy and calving resolution. Endometrial cytology is a noninvasive technique with high specificity and repeatability for the diagnosis of subclinical endometritis postpartum in dystocic beef heifers.

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Section: Introductionmentioning

confidence: 99%

Endometrial cytology as a diagnostic tool for subclinical endometritis in beef heifers

Moscuzza

Alvarez²,

Gutierrez³

et al. 2015

Turk J Vet Anim Sci

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“…Chapwanya et al, (2009) reported that the severity of uterine inflammation mirrors the molecular expression of inflammatory genes. The results from Experiment 1 suggest that collecting a single EB from either the injured or non-injured horn is indicative of endometrial health.…”

Section: Discussionmentioning

confidence: 99%

“…However, the recovery of the endometrial sample does not always guarantee that all the biopsies are readable or considered of good quality. For the diagnosis of endometritis in cattle, the pathologist assesses the epithelium continuity and infiltration of inflammatory cells, vascular congestion, the integrity of uterine glands and cellular infiltration in submucosa layers (Chapwanya et al, 2009, Meira et al, 2012. Thus, if one of these components is absent, the sample is considered unreadable and difficult to interpreted (Bonnett et al, 1991a).…”

Section: Collection Of Endometrial Tissuementioning

confidence: 99%

“…This procedure allows the morphological diagnosis of endometrial lesions by histopathology (Chapwanya et al, 2009, Meira et al, 2012, the expression of inflammatory genes during the oestrous cycle or postpartum , Galvao et al, 2011 and the detection of biomarkers and pathways responsible for endometrial receptivity and pregnancy success by means of molecular techniques (Salilew-Wondim et al, 2010, Salilew-Wondim et al, 2012. To ensure successful pregnancy outcomes from AI and ET technologies, assuring high heifer fertility is optimal is crucial for a successful breeding season.…”

Section: Introductionmentioning

confidence: 99%

“…Histologically, endometritis is characterised by increased infiltration of inflammatory cells associated with glandular and vascular changes (Chapwanya et al, 2010). Although the diagnosis of endometritis by histopathology is not difficult, defined criteria and uniformity in the mode of assessment were lacking in more subjective assessment methods (Singh et al, 1983, Bonnett et al, 1991b, McDougall et al, 2007, Chapwanya et al, 2009 Questions remain as to the impact of endometrial biopsy on later fertility and this study aims to assess endometrial morphological changes in heifers after biopsy using the small jaw Storz® device. A biopsy was taken at Day 7 post-oestrus of the preceding cycle or at Day 4 of the ongoing cycle then assessment of microscopic changes on Day 7 after oestrus (either 10 or 3 days after biopsy).…”

Section: Introductionmentioning

confidence: 99%

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Effect of endometrial biopsy on uterine health in tropically adapted beef cattle

Garzon¹,

Orlando²

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In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

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Uterine Infection in Bovines: An Update

Kumaresan

Praveen

Manimaran

et al. 2022

Current Concepts in Bovine Reproduction

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Histopathological and molecular evaluation of Holstein-Friesian cows postpartum: Toward an improved understanding of uterine innate immunity

Cited by 132 publications

References 43 publications

Endometrial cytology as a diagnostic tool for subclinical endometritis in beef heifers

Endometrial cytology as a diagnostic tool for subclinical endometritis in beef heifers

Effect of endometrial biopsy on uterine health in tropically adapted beef cattle

Uterine Infection in Bovines: An Update

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