Histopathological abnormalities of prolonged alloxan‐induced diabetes mellitus in rabbits

Abstract

The objective of this study was to investigate the prolonged complications of untreated diabetes on histomorphology of rabbits. Diabetes mellitus was experimentally induced in one group of New Zealand white male rabbits by intraperitoneal administration of four doses of alloxan @ 80 mg/kg b.w. at weekly intervals following 12 h fasting. Other group of rabbits served as healthy controls that received isotonic saline in a similar manner. The establishment of diabetes mellitus was confirmed by fasting blood glucose levels. For histomorphological study of different organs, 50% of the animals were killed after 7 weeks and the rest after 26 weeks. Routine haematoxylin and eosin stain and Gomori's modified stain were used. The blood glucose level of diabetic rabbits increased significantly throughout the experimental period. The peak values for blood sugar were on the sixth week of the study. Further, histomorphological alterations were recorded in pancreas, kidneys, lungs, heart and brain in diabetic rabbits. However, mild changes were observed in gastrointestinal tract with proliferation of yeasts in the stomach. With the progress of untreated diabetes, the histoanatomical alterations intensify and extend to almost all organs of the body and appear to increase the susceptibility of gastric mucosa to yeast cell proliferation.

Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats

“…The liver plays a significant role in maintaining glucose and fat metabolism [37]. It is reported that some degenerative changes in hepatocyte are lipolysis and vacuolation [31,33,38]. Our study observed, similar to other studies, lipid accumulation in liver due to degenerative changes and fat vacuoles in the cytoplasm of hepatocytes (Fig.…”

“…Hystopathological examination of the pancreas in the diabetic group (Fig. 3c-d) indicated the mononuclear cell infiltration of interlobular septum and vacuolizations depended on glycogen degeneration in the ductal epithelium and islets of Langerhans, in similar to previous studies [31][32][33].…”

The Association between Diffuse Myocardial Fibrosis on Cardiac Magnetic Resonance T1 Mapping and Myocardial Dysfunction in Diabetic Rabbits

“…Diabetic rabbits are known to exhibit myocardial fibrosis1314. Therefore, in this study, we established a diabetic rabbit model and continuously observed the changes in cardiac function and the degree of diffuse interstitial fibrosis.…”

Impaired glucose tolerance plus hyperlipidaemia induced by diet promotes retina microaneurysms in New Zealand rabbits

“…2001; Sochor et al. 1979; Mir & Darzi 2009). Retinopathy seems to be a consequence of glucose toxicity and increased oxidative stress.…”