Histopathologic parameters in chronic rhinosinusitis with nasal polyposis: impact on quality of life outcomes

Soy, Fatih Kemal; Pınar, Ercan; İmre, Abdülkadir; Çallı, Çağlar; Çallı, Aylin Örgen; Öncel, Semih

doi:10.1002/alr.21183

Cited by 30 publications

(39 citation statements)

References 25 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Eosinophils are a major inflammatory cell in eosinophilic rhinosinusitis, 36 a more severe form of sinonasal inflammation that is proving difficult to treat. 37,38 Mast cells were found throughout the OE epithelium after LL-37 treatment in a dose dependent manner. Interestingly, both LL-37 and mast cells are both highly elevated in patients with sinonasal inflammation.…”

Section: Discussionmentioning

confidence: 92%

Topical cathelicidin (LL‐37) an innate immune peptide induces acute olfactory epithelium inflammation in a mouse model

Alt

Qin

Pulsipher

et al. 2015

Int Forum Allergy Rhinol

View full text Add to dashboard Cite

Background Cathelicidin (LL-37) is an endogenous innate immune peptide that is elevated in patients with chronic rhinosinusitis (CRS). The role of LL-37 in olfactory epithelium (OE) inflammation remains unknown. We hypothesized that 1) LL-37 topically delivered would elicit profound OE inflammation, and 2) LL-37 induced inflammation is associated with increased infiltration of neutrophils and mast cells. Methods To test our hypothesis we challenged C57BL/6 mice intranasally with increasing concentrations of LL-37. At 24 hours tissues were examined histologically and scored for inflammatory cell infiltrate, edema, and secretory hyperplasia. In separate experiments, fluorescently conjugated LL-37 was instilled and tissues were examined at 0.5 and 24 hours. To test our last hypothesis, we performed tissue myeloperoxidase (MPO) assays for neutrophil activity and immunohistochemistry for tryptase to determine the mean number of mast cells per mm2. Results LL-37 caused increased inflammatory cell infiltrate, edema, and secretory cell hyperplasia of the sinonasal mucosa with higher LL-37 concentrations yielding significantly more inflammatory changes (p < 0.01). Fluorescent LL-37 demonstrated global sinonasal epithelial binding and tissue distribution. Further, higher concentrations of LL-37 led to significantly greater MPO levels with dose-dependent increases in mast cell infiltration (p < 0.01). Conclusions LL-37 has dramatic inflammatory effects in the OE mucosa that is dose-dependent. The observed inflammatory changes in the olfactory mucosa were associated with the infiltration of both neutrophils and mast cells. Our biologic model represents a new model to further investigate the role of LL-37 in OE inflammation.

show abstract

Section: Discussionmentioning

confidence: 92%

Topical cathelicidin (LL‐37) an innate immune peptide induces acute olfactory epithelium inflammation in a mouse model

Alt

Qin

Pulsipher

et al. 2015

Int Forum Allergy Rhinol

View full text Add to dashboard Cite

show abstract

“…The presence of goblet cells and subepithelial thickening in perioperative specimens from CRS patients correlates with some bothersome nasal symptoms after ESS [49]. The thickening of the basal membrane adversely affects the patients' quality of life, as measured by the Rhinosinusitis Disability Index (RDI) and the Short Form-36 (SF-36) General Health Survey quality of life questionnaires, and correlates with the disease severity in patients with CRS [50]. However, although information that is obtainable from light microscopy can serve as valuable prognostic indicators, routine H&E staining is suboptimal in distinguishing among the various CRS subgroups [79].…”

Section: Biological Markers Of Difficult-to-treat Severe Chronic Rhinmentioning

confidence: 99%

Clinical and Biological Markers of Difficult-to-Treat Severe Chronic Rhinosinusitis

López-Chacón

Mullol

Pujols

2015

Curr Allergy Asthma Rep

View full text Add to dashboard Cite

Chronic rhinosinusitis (CRS) is a chronic inflammatory disease of the upper airways of which two major phenotypes exist, CRS without nasal polyps (CRSsNP) and CRS with nasal polyps (CRSwNP). Some patients with CRS have suboptimal response to current guideline treatments. These patients remain severe and uncontrolled by treatment and have a poor quality of life. It is highly important to identify both clinical and biological markers, so-called biomarkers, in this subset of patients. The presence of nasal polyps and comorbidity with asthma and with aspirin-exacerbated respiratory disease (AERD) are the most common clinical traits that have been associated to difficult-to-treat severe CRS. In addition to clinical traits, numerous biological markers, with known etiopathogenic roles in CRS, have been associated to difficult-to-treat or recalcitrant CRS. This review summarizes the existing knowledge of the clinical and biological markers associated to difficult-to-treat or uncontrolled severe CRS.

show abstract

“…Rhinosinusitis is a common medical condition with remarkable symptoms and a noticeable impact on quality of life . The term rhinosinusitis reflects the concurrent inflammatory and infectious processes that affect the nasal passages and the contiguous paranasal sinuses …”

Section: Introductionmentioning

confidence: 99%

“…Documentation of persistent mucosal inflammation is the key to the diagnosis of CRS. In cases of CRS, it has been shown that pathological changes occur in the ciliary epithelium …”

Section: Introductionmentioning

confidence: 99%

Treatment of rhinosinusitis and histopathology of nasal mucosa: A controlled, randomized, clinical study

et al. 2016

View full text Add to dashboard Cite

show abstract

Histopathologic parameters in chronic rhinosinusitis with nasal polyposis: impact on quality of life outcomes

Abstract: For most patients QOL significantly improved after surgery. Mucosal eosinophilia did not correlate with the absolute change of the RSDI. However, thickening of basal membrane adversely affects symptoms of the patients and correlates with the disease severity.

Cited by 30 publications

References 25 publications

Topical cathelicidin (LL‐37) an innate immune peptide induces acute olfactory epithelium inflammation in a mouse model

Topical cathelicidin (LL‐37) an innate immune peptide induces acute olfactory epithelium inflammation in a mouse model

Clinical and Biological Markers of Difficult-to-Treat Severe Chronic Rhinosinusitis

Treatment of rhinosinusitis and histopathology of nasal mucosa: A controlled, randomized, clinical study

Contact Info

Product

Resources

About