Histopathologic Findings Following Experimental Equine Herpesvirus 1 Infection of Horses

Abstract

Histopathological differences in horses infected with equine herpesvirus type 1 (EHV-1) of differing neuropathogenic potential [wild-type (Ab4), polymerase mutant (Ab4 N752), EHV-1/4 gD mutant (Ab4 gD4)] were evaluated to examine the impact of viral factors on clinical disease, tissue tropism and pathology. Three of 8 Ab4 infected horses developed Equine Herpesvirus Myeloencephalopathy (EHM) requiring euthanasia of 2 horses on day 9 post-infection. None of the other horses showed neurologic signs and all remaining animals were sacrificed 10 weeks post-infection. EHM horses had lymphohistiocytic vasculitis and lymphocytic infiltrates in the lungs, spinal cord, endometrium and eyes. EHV-1 antigen was detected within the eyes and spinal cord. In 3/6 of the remaining Ab4 infected horses, 4/9 Ab4 N752 infected horses, and 8/8 Ab4 gD4 infected horses, choroiditis was observed. All males had interstitial lymphoplasmacytic and/or histiocytic orchitis and EHV-1 antigen was detected. In conclusion, only animals sacrificed due to EHM developed overt vasculitis in the CNS and the eye. Mild choroiditis persisted in many animals and appeared to be more common in Ab4 gD4 infected animals. Finally, we report infiltrates and changes in the reproductive organs of all males associated with EHV-1 antigen. While the exact significance of these changes is unclear, these findings raise concern for long-term effects on reproduction and prolonged shedding of virus through semen.

Respiratory and Reproductive Tract Disorders Causing Herpesviruses in Animals

“…Other lesions include lung edema, thymic and hepatic necrosis; haemorrhages on various organs (myocardium, kidneys and spleen). Histologic pictures revealed also the presence of Intranuclear inclusion bodies in above mentioned tissues (Holz et al 2019).…”

Archaic connectivity between the sulfated heparan sulfate and the herpesviruses – An evolutionary potential for cross-species interactions

“…Other clinical studies provide varying evidence for the specific effects of HSV infection on sperm parameters and highlight that these effects may be exacerbated by common antiviral treatments [83] , [84] , [85] , [86] . Even so, the vulnerability of male reproductive organs or function and HSV infection has been documented in several mammalian species, including mice [87] , [88] , horses [89] , and dogs [90] . Where species appear to differ from one another is the manifestation of reproductive toxicity, with some mammalian species showing direct testicular damage, others show changes primarily in the health, quantity, or viability of sperm.…”

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infection with Equine Herpesvirus‐1

“…After experimental infection with the so-called neuropathogenic strains, an incidence of neurological sign of 25% (9/36), 36% (5/14) or 38% (3/8) was found (Allen, 2008;Brosnahan et al, 2012;Holz et al, 2019), but the small number of involved horses can bias the results.…”