Histopathologic Biomarkers

Hinton, David E.; Baumann, Paul C.; Gardner, George R.; Hawkins, William E.; Hendricks, Jerry D.; Murchelano, Robert A.; Okihiro, Mark S.

doi:10.1201/9781351070270-5

Cited by 41 publications

(26 citation statements)

References 1 publication

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“…An original and innovative objective of this study was to explore whether individuals with hepatic lesions had lower metabolites' levels, because of a less efficiency of their liver to metabolize phthalate parents. The prevalent histopathological characteristics identified in eel from Mediterranean lagoons were hepatocellular necrosis and parenchymal lysis, cellular hyperplasia associated to lipid vacuolization and fibrosis who are interpreted as nonspecific responses to stress as they have been described in fish exposed to a wide spectrum of pollutants (Hinton et al, 1992). The lipoid degeneration (fatty liver) is one of the major changes observed here.…”

Section: Histological Abnormalitiessupporting

confidence: 61%

Phthalate metabolites in the European eel (Anguilla anguilla) from Mediterranean coastal lagoons

Fourgous

Chevreuil

Alliot

et al. 2016

Science of The Total Environment

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The levels and fate of phthalate metabolites have been poorly evaluated in fish, despite their potential ecotoxicological impacts. The present study aims to characterize the levels of phthalate metabolites in muscle tissue of yellow eels (Anguilla anguilla) from two coastal Mediterranean lagoons, during three sampling periods. Nine phthalate metabolites were detected in >70% of the samples. Slightly higher levels of phthalate metabolites were detected in March and June compared to October, suggesting possible seasonal variations in environmental release and/or phthalate metabolization process by eels. The large sample size (N=117) made it possible to explore correlations between phthalate metabolites' levels and individual parameters, such as body length, age, body condition and hepatic histo-pathologies. Body length and estimated age poorly correlated with phthalate metabolites, suggesting that eels did not accumulate phthalates during growth, contrary to persistent compounds. Eels presented different grades of hepatic fibrosis and lipidosis. A negative correlation was found between the severity of these pathologies in the liver and the sum of phthalate metabolites levels, supporting the hypothesis that eels with damaged liver are less able to metabolize xenobiotics.

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Section: Histological Abnormalitiessupporting

confidence: 61%

Phthalate metabolites in the European eel (Anguilla anguilla) from Mediterranean coastal lagoons

Fourgous

Chevreuil

Alliot

et al. 2016

Science of The Total Environment

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“…Histopathological alterations such as lesions and tissue disturbances in the liver, ovary, skeleton, and skin have been used as common biomarkers in ecotoxicological studies . Ezemonye and Enuneku reported excessive bile secretion and dilation of sinusoids in the liver of Hoplobatrachus occipitalis exposed to Cd and hemorrhages in the liver of Bufo maculatus exposed to lead.…”

Section: Introductionmentioning

confidence: 99%

Heavy metal–induced toxicity in the Indian green frog: Biochemical and histopathological alterations

Jayawardena

Angunawela

Wickramasinghe

et al. 2017

Enviro Toxic and Chemistry

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Heavy metal contamination may have adverse effects on wetland biota, particularly on amphibians. Severe immunotoxic effects elicited in Euphlyctis hexadactylus (Indian green frog) because of metal exposure (Cd, Cr, Cu, Pb, and Zn) in the Bellanwila-Attidiya Sanctuary, a polluted urban wetland in Sri Lanka, provided the rationale for the present study. We evaluated the biochemical and histopathological effects of this metal contamination with a reference E. hexadactylus population and a laboratory exposure group that was subjected to 28 d of exposure to a mixture of Cd, Cr, Cu, Pb, and Zn (5 ppm in each mixture). A histopathological scoring for the semiquantification of tissue damage was established. Results of the biochemical and histopathological markers were remarkably consistent between the 2 exposure scenarios, providing validation for the heavy metal exposure hypothesis. Damage to liver, kidney, lung, and skin of metal-exposed E. hexadactylus quantified multiple impairments absent in the reference frogs. Liver injuries complemented significantly elevated aspartate transaminase (AST), alanine transaminase (ALT), γ-glutamyl transferase (γ-GT), and alkaline phosphatases in frog liver homogenate, indicating hepatocellular leakage and loss of functional and structural integrity of the hepatocyte membrane in both field- and laboratory-exposed frogs. Significant elevation of Kupffer cell hypertrophy, pigmentation, inflammatory cell infiltrates and hepatic inflammation, extramedullary hematopoiesis, karyocytomegaly of hepatocytes (p < 0.05) of the liver, and degeneration of epithelia and necrosis of the lung, manifested as impairments in both metal exposure scenarios. Significantly reduced serum total protein and albumin and significantly elevated urea and creatinine in metal-exposed frogs were indicative of hepatic and renal dysfunction, respectively. The present study affirms histopathology-related biochemical alterations as potential biomarkers for heavy metal toxicity in amphibians. Environ Toxicol Chem 2017;36:2855-2867. © 2017 SETAC.

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“…Histopathological alternations are biomarkers of the effect of exposure to environmental stressors, revealing prior alternations in physiological and/or biochemical function (Hinton et al, 1992). Tissue injuries and damages in organs can result in the reduced survival, growth and fitness, the low reproductive success or increase of susceptibility to pathological agents.…”

Section: Introductionmentioning

confidence: 99%

Histopathological Changes in the Liver of Etroplus Suratensis (Pearlspot) Exposed to Selected Insecticide, Lambda-Cyhalothrin

Vidhya¹,

Tjprc²

2019

IJZR

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Histopathological investigations on different tissues of fish are valuable tools for toxicology studies and monitoring water pollutions. Contamination of water by pesticides, either directly or indirectly, can lead to fish kills, reduced fish productivity, or elevated concentrations of undesirable chemicals in edible fish tissue which can affect the health of humans consuming these fish. Fishes were randomly selected for histopathological observations by sampling after 60 days of pesticide exposure. No histopathological effects were observed in the control group. Many alterations in the liver such as necrosis, damaged hepatocytes, lymphatic aggregation, degeneration, rupture of the hepatocytes, haemorrhage, coagulative necrosis, accumulation of blood cells, displacement of the nucleus and completely damaged hepatic cells were noticed in pesticide-treated groups.

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Histopathologic Biomarkers

Cited by 41 publications

References 1 publication

Phthalate metabolites in the European eel (Anguilla anguilla) from Mediterranean coastal lagoons

Phthalate metabolites in the European eel (Anguilla anguilla) from Mediterranean coastal lagoons

Heavy metal–induced toxicity in the Indian green frog: Biochemical and histopathological alterations

Histopathological Changes in the Liver of Etroplus Suratensis (Pearlspot) Exposed to Selected Insecticide, Lambda-Cyhalothrin

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