Histopathologic and Ultrastructural Changes in Seminiferous Tubules of Adult Male Albino Rats Following Daily Administration of Different Doses of Tadalafil

Eid, Abulfotooh M.; Dine, Fatma Mohamed Magdy Badr El; Nabil, Iman

doi:10.1016/j.urology.2015.12.038

Cited by 6 publications

(7 citation statements)

References 27 publications

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“…Therefore, further studies are warranted to elucidate the optimal dose and duration of tadala l administration to maximize e cacy without possible adverse effects. Furthermore, there is concern regarding the long-term use of PDE5is because some reports have shown that chronic administration of tadala l to rats may result in degenerative changes in the testis and a decline in semen parameters [55]. Therefore, additional studies regarding the long-term adverse effects of tadala l itself on the normal or pathological testes will be required.…”

Section: Discussionmentioning

confidence: 99%

The long-term protective effect of tadalafil on spermatogenesis following testicular ischemia-reperfusion injury in a rat model

Kim¹,

Lee²,

Yoon³

et al. 2020

Preprint

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Background Testicular torsion is a urological emergency in which misdiagnosis and inappropriate treatment can lead to testicular atrophy and male infertility owing to ischemia-reperfusion injury (IRI). Although experimental studies of testicular torsion have been preceded, promising therapeutic agents based on the long-term effect for spermatogenesis have not been identified in testicular ischemia reperfusion injury (IRI) animal model. Tadalafil, one of the phosphodiesterase-5 inhibitors commonly used in the treatment of erectile dysfunction, has recently reported a protective effect against IRI in several organs. In this study, we evaluated the long-term protective effect of tadalafil for spermatogenesis in a rat testicular IRI model. Methods Forty-eight adolescent Sprague–Dawley rats were divided into 6 groups (A-F). Sham operation was performed in group A. Group B received surgical 720-degree torsion of the left testis without any medication. Groups C, D, E, and F were operated surgical torsion with tadalafil at varying doses (0.3 mg/kg, 1.0 mg/kg) and durations (single or daily administration for 4 weeks). Detorsion was performed after 3 hour of torsion in all rats except the sham group. Four weeks after operation, both testes were evaluated of spermatogenesis using Johnsen scoring. To evaluate the protective effect of tadalafil against oxidative stress by IRI, malondialdehyde (MDA) and superoxide dismutase (SOD) level were analyzed via ELISA in both testes 4 hour after detorsion in the same experiments as in group A, B, and C. Results For the evaluation of spermatogenesis according to doses, the groups with high-dose tadalafil showed a higher Johnsen scores than low-dose counterparts. The groups with daily administration for 4weeks were observed a higher Johnsen scores than those given a single administration. Furthermore, molecular markers (MDA and SOD) related with oxidative stress and histopathologic findings showed remarkable improvement after tadalafil administration. Conclusion Tadalafil alleviated long-term deterioration of spermatogenesis and oxidative stress by restoring antioxidant status after testicular IRI rat model. Furthermore, it demonstrated a protective effect against testicular IRI in a time- and dose-dependent manner.

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Section: Discussionmentioning

confidence: 99%

The long-term protective effect of tadalafil on spermatogenesis following testicular ischemia-reperfusion injury in a rat model

Kim¹,

Lee²,

Yoon³

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…In the experimental model, it has been documented that rats exposed to increasing doses for 12 weeks (equivalent of 5, 10, and 20 mg daily) showed worsening of sperm parameters and a dose-dependent histopathological degenerative change of the seminiferous tubules and also in Johnsen's score (qualitative evaluation of spermatogenesis) was significantly lower in the animal treated with a dose of 1.8 mg/kg (equivalent to 20 mg) [15]. …”

Section: Discussionmentioning

confidence: 99%

“…The results of our study are not comparable for different reasons: different doses used (5 mg versus 20 mg), duration of the treatment (six months versus acute administration), and different clinical model (in our study, we enrolled patients with MAGI). In pathophysiological terms, the different modulations of sperm motility obtained 1 and 2 hours after administration suggest a pharmacological mechanism that could involve the secretion of prostate and seminal vesicles and not the epididymal phase of maturation [15]. Moreover, it should also be considered that Tadalafil in the therapeutic range plays also an inhibitory role on isoenzyme 11, involved in the modulation of spermatogenesis [17].…”

Section: Discussionmentioning

confidence: 99%

Chronic Administration of Tadalafil Improves the Symptoms of Patients with Amicrobic MAGI: An Open Study

Vignera

Condorelli

Mongioì

et al. 2017

International Journal of Endocrinology

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Aim of this study was to evaluate the effects of pharmacological treatment with Tadalafil 5 mg daily on symptoms and quality of sperm parameters in selected patients with amicrobic MAGI (male accessory gland inflammation). 120 patients with amicrobic MAGI (mean age 27.0 ± 6.0 years) with mild-moderate ED (erectile dysfunction) according to IIEF-5 (International Index of Erectile Function 5 Items) scores underwent pharmacological treatment with Tadalafil 5 mg daily for six months. Before and after treatment these patients were evaluated through IIEF-5, semen analysis (according to WHO Criteria, 2010), SI-MAGI (Structured Interview about Male Accessory Gland Inflammation), and ultrasound evaluation. Patients with PVE (prostate-vesciculo-epididymitis) showed a significant increase in the percentage of spermatozoa with total (16.0 ± 8.0 versus 30.0 ± 6.0%) and progressive motility (8.00 ± 10.0 versus 25.0 ± 6.00%). It was a significant reduction of the number of patients with complicated ultrasound forms (30.0 versus 52.0) and a significant increase of the number of patients with uncomplicated ultrasound form (90.0 versus 68.0). Finally, there was a significant reduction in the percentage of patients with alterations of sexual function different from DE, such as premature ejaculation (4.00 versus 8.00%), painful ejaculation (4.00 versus 10.0%), delayed ejaculation (12.50 versus 8.00%), and decreased libido (10.0 versus 25.0%).

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“…118,119 Khalaf and co-workers showed that administration of tadalafil daily for 6 months in rats significantly reduced spermatogenesis and quantitative sperm motility. 120 Another study 121 in adult Wister rats reported that daily tadalafil treatment resulted in increased alertness, and that compared with placebo or a 5 mg dose, sperm motility/ morphology decreased significantly in animals treated with equivalent human doses of 10 mg (0.9 mg/kg) or 20 mg (1.8 mg/kg). The 20 mg dose reduced sperm count substantially.…”

Section: Pde5is and Spermatozoal Qualitative And Quantitative Paramet...mentioning

confidence: 99%

“…The 20 mg dose reduced sperm count substantially. 121 A study on 200 men with or without erectile dysfunction double-blindly randomized to daily use of vardenafil (20 mg), sildenafil (100 mg), or placebo for 6 months has concluded that vardenafil does not impair sperm concentration, total sperm count per ejaculate, sperm morphology, motility, or reproductive hormones levels. 122 Findings in our laboratory 37 have suggested very vividly that in vivo administration of avanafil either alone either in combination with micronutrient supplements increase sperm membrane permeability and subsequently sperm ability to undergo hyperactivation under biochemical conditions known to induce sperm capacitation.…”

Section: Pde5is and Spermatozoal Qualitative And Quantitative Paramet...mentioning

confidence: 99%

PDE5 inhibitors and male reproduction: Is there a place for PDE5 inhibitors in infertility clinics or andrology laboratories?

et al. 2022

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The objective of this review study is to evaluate the therapeutic role of PDE5 inhibitors (PDE5is) in the amelioration of oligoasthenospermia in infertile males. PDE5is have a beneficial influence on the secretory function of the Leydig and Sertoli cells, the biochemical environment within the seminiferous tubule, the contractility of the testicular tunica albuginea, and the prostatic secretory function. In several studies, the overall effect of sildenafil and vardenafil increased quantitative and qualitative sperm motility. Furthermore, some studies indicate that PDE5is influence positively the sperm capacity to undergo capacitation under biochemical conditions that are known to induce the sperm capacitation process. Additional research efforts are necessary in order to recommend unequivocally the usage of sildenafil, vardenafil, or avanafil for the alleviation of male infertility.

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Histopathologic and Ultrastructural Changes in Seminiferous Tubules of Adult Male Albino Rats Following Daily Administration of Different Doses of Tadalafil

Cited by 6 publications

References 27 publications

The long-term protective effect of tadalafil on spermatogenesis following testicular ischemia-reperfusion injury in a rat model

The long-term protective effect of tadalafil on spermatogenesis following testicular ischemia-reperfusion injury in a rat model

Chronic Administration of Tadalafil Improves the Symptoms of Patients with Amicrobic MAGI: An Open Study

PDE5 inhibitors and male reproduction: Is there a place for PDE5 inhibitors in infertility clinics or andrology laboratories?

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