2021
DOI: 10.1038/s42003-021-01707-z
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Histone variant H2A.B-H2B dimers are spontaneously exchanged with canonical H2A-H2B in the nucleosome

Abstract: H2A.B is an evolutionarily distant histone H2A variant that accumulates on DNA repair sites, DNA replication sites, and actively transcribing regions in genomes. In cells, H2A.B exchanges rapidly in chromatin, but the mechanism has remained enigmatic. In the present study, we found that the H2A.B-H2B dimer incorporated within the nucleosome exchanges with the canonical H2A-H2B dimer without assistance from additional factors, such as histone chaperones and nucleosome remodelers. High-speed atomic force microsc… Show more

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Cited by 17 publications
(5 citation statements)
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References 72 publications
(125 reference statements)
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“…Deposition pattern of H2A.Bbd mostly overlaps with regions marked by H4 acetylation, indicating an active gene transcription region in the genome [ 173 , 174 ]. In addition to that, H2A.Bbd-H2B dimer is spontaneously exchanged within nucleosome with the assistance of a NAP1 histone chaperone [ 175 , 176 ]. Functionally, recent reports have shown that knockdown of H2A.Bbd resulted in an altered chromatin structure in male mouse’s post-meiotic germ cells, yet without affecting spermatogenesis [ 174 , 177 , 178 ].…”
Section: The 19 Histone H2a Variantsmentioning
confidence: 99%
“…Deposition pattern of H2A.Bbd mostly overlaps with regions marked by H4 acetylation, indicating an active gene transcription region in the genome [ 173 , 174 ]. In addition to that, H2A.Bbd-H2B dimer is spontaneously exchanged within nucleosome with the assistance of a NAP1 histone chaperone [ 175 , 176 ]. Functionally, recent reports have shown that knockdown of H2A.Bbd resulted in an altered chromatin structure in male mouse’s post-meiotic germ cells, yet without affecting spermatogenesis [ 174 , 177 , 178 ].…”
Section: The 19 Histone H2a Variantsmentioning
confidence: 99%
“…Nucleosome composition in turn affects the binding dynamics of distinct histone subunits in specific regions. For instance, H2A/H2B heterodimers exchange more readily as compared to H3/H4 tetramers [18][19][20] .…”
Section: Mainmentioning
confidence: 99%
“…Such a histone chaperone function of the nucleosome would result in stabilized nucleosomes at the cost of freely and spontaneously exchanged histones, although spontaneous histone exchange between nucleosomes has never been reported. To support this hypothesis, spontaneous exchange of nucleosomal H2A-H2B with free H2A-H2B has been reported ( 16 , 21 , 22 ). Histone exchange among nucleosomes on a relevant timescale would imply that histone modifications and variants can diffuse and redistribute themselves spontaneously in chromatin after serving their purpose.…”
mentioning
confidence: 93%