Histone Methyltransferase aflrmtA gene is involved in the morphogenesis, mycotoxin biosynthesis, and pathogenicity of Aspergillus flavus

Liu, Yu; He, Yizi; Li, Xing; Fasoyin, Opemipo Esther; Hu, Yule; Liu, Yaju; Yuan, Jun; Zhang, Zhuang; Wang, Shihua

doi:10.1016/j.toxicon.2017.01.013

Cited by 25 publications

(28 citation statements)

References 31 publications

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“…flavus infection of peanut and maize seeds [24]. Recent studies also showed that histone modification, including the histone acetyltransferase AflGcnE [79] and the histone methyltransferase aflrmtA [80], affect the A . flavus pathogenicity during colonization of maize and peanut seeds.…”

Section: Resultsmentioning

confidence: 99%

Proteome-wide profiling of protein lysine acetylation in Aspergillus flavus

2017

PLoS ONE

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Protein lysine acetylation is a prevalent post-translational modification that plays pivotal roles in various biological processes in both prokaryotes and eukaryotes. Aspergillus flavus, as an aflatoxin-producing fungus, has attracted tremendous attention due to its health impact on agricultural commodities. Here, we performed the first lysine-acetylome mapping in this filamentous fungus using immune-affinity-based purification integrated with high-resolution mass spectrometry. Overall, we identified 1383 lysine-acetylation sites in 652 acetylated proteins, which account for 5.18% of the total proteins in A. flavus. According to bioinformatics analysis, the acetylated proteins are involved in various cellular processes involving the ribosome, carbon metabolism, antibiotic biosynthesis, secondary metabolites, and the citrate cycle and are distributed in diverse subcellular locations. Additionally, we demonstrated for the first time the acetylation of fatty acid synthase α and β encoded by aflA and aflB involved in the aflatoxin-biosynthesis pathway (cluster 54), as well as backbone enzymes from secondary metabolite clusters 20 and 21 encoded by AFLA_062860 and AFLA_064240, suggesting important roles for acetylation associated with these processes. Our findings illustrating abundant lysine acetylation in A. flavus expand our understanding of the fungal acetylome and provided insight into the regulatory roles of acetylation in secondary metabolism.

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Section: Resultsmentioning

confidence: 99%

Proteome-wide profiling of protein lysine acetylation in Aspergillus flavus

2017

PLoS ONE

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“…Recently, it has been demonstrated that there is a biological relationship between DNA and histone methylation, which both are correlated with different chemical reactions. This relationship has a vital function in gene silencing from fungi to mammals [96][97][98][99][100]. The histone modification markers, such as H3K4me3, may serve as indirect regulators to influence DNA methylation [61,101].…”

Section: Dna Methylation and Histone Methylation In Fungimentioning

confidence: 99%

“…PRMT5 is the primary methyltransferase in charge of histone arginine methylation (H3R8me), and the decreased levels of PRMT5 can diminish the binding between DNMT3A and chromatin, reduce DNA methylation, and subsequently facilitate genetic transcription [98]. Current evidence suggests that DNA and histone methylation commonly regulate fungal development and mycotoxin biosynthesis [99]. Histone methylation is readily reversible and generally precedes DNA methylation in N. crassa, while DNA methylation is relatively stable and conduces to form a stable heterochromatic state [97,100].…”

Section: Dna Methylation and Histone Methylation In Fungimentioning

confidence: 99%

The Pattern and Function of DNA Methylation in Fungal Plant Pathogens

Zhang

et al. 2020

Microorganisms

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To successfully infect plants and trigger disease, fungal plant pathogens use various strategies that are dependent on characteristics of their biology and genomes. Although pathogenic fungi are different from animals and plants in the genomic heritability, sequence feature, and epigenetic modification, an increasing number of phytopathogenic fungi have been demonstrated to share DNA methyltransferases (MTases) responsible for DNA methylation with animals and plants. Fungal plant pathogens predominantly possess four types of DNA MTase homologs, including DIM-2, DNMT1, DNMT5, and RID. Numerous studies have indicated that DNA methylation in phytopathogenic fungi mainly distributes in transposable elements (TEs), gene promoter regions, and the repetitive DNA sequences. As an important and heritable epigenetic modification, DNA methylation is associated with silencing of gene expression and transposon, and it is responsible for a wide range of biological phenomena in fungi. This review highlights the relevant reports and insights into the important roles of DNA methylation in the modulation of development, pathogenicity, and secondary metabolism of fungal plant pathogens. Recent evidences prove that there are massive links between DNA and histone methylation in fungi, and they commonly regulate fungal development and mycotoxin biosynthesis.

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“…(Satterlee et al, 2016) aflrmtA Histone methyltransferase gene aflrmtA represses conidiation and up-regulates the AFB 1 biosynthesis in A. flavus on peanut and corn seeds as well as protease and lipase activities, but downregulates the amylase activity. (Li et al, 2017) nsdC The transcription factor required for normal asexual development and AF production in A. flavus. RNA sequencing analysis of the nsdC knockout mutant and isogenic control strain identified a number of differentially transcribed genes related to development and production of secondary metabolites, including AF, penicillin and aflatrem.…”

Section: Gene Function Referencementioning

confidence: 99%

Aflatoxins: biosynthesis, prevention and eradication

Šimončicová

Kaliňáková

Kryštofová

2017

Acta Chimica Slovaca

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Filamentous fungi belonging to Aspergilli genera produce many compounds through various biosynthetic pathways. These compounds include a spectrum of products with beneficial medical properties (lovastatin) as well as those that are toxic and/or carcinogenic which are called mycotoxins. Aspergillus flavus, one of the most abundant soil-borne fungi, is a saprobe that is able growing on many organic nutrient sources, such as peanuts, corn and cotton seed. In many countries, food contamination by A. flavus is a huge problem, mainly due to the production of the most toxic and carcinogenic compounds known as aflatoxins. In this paper, we briefly cover current progress in aflatoxin biosynthesis and regulation, pre-and postharvest preventive measures, and decontamination procedures.

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Histone Methyltransferase aflrmtA gene is involved in the morphogenesis, mycotoxin biosynthesis, and pathogenicity of Aspergillus flavus

Cited by 25 publications

References 31 publications

Proteome-wide profiling of protein lysine acetylation in Aspergillus flavus

Proteome-wide profiling of protein lysine acetylation in Aspergillus flavus

The Pattern and Function of DNA Methylation in Fungal Plant Pathogens

Aflatoxins: biosynthesis, prevention and eradication

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