Histone lysine methylation exhibits a distinct distribution during spermatogenesis in pigs

An, Junhui; Qin, Jinzhou; Wan, Yi Ching Esther; Zhang, Yaqing; Hu, Yuan; Zhang, Chunfang

doi:10.1016/j.theriogenology.2015.07.013

Cited by 14 publications

(10 citation statements)

References 28 publications

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“…Male germline cells drastically change their morphology during spermatogenesis. Previous studies have shown the localization of H3K79me2/3 in spermatocytes and spermatids as well as the accumulation of H3K4me, H3K9me and H3K27me during transformation from spermatogonia to elongated spermatids [34,35,[44][45][46][47]; however, comprehensive detailed subcellular localization patterns have not been fully examined. Here, we first investigated the localization of major histone modifications from preleptotene spermatocytes to elongated spermatids according to 12 stages based on a previous report [48].…”

Section: Morphological Profiles Showing Histone Modification Patternsmentioning

confidence: 99%

Comprehensive histochemical profiles of histone modification in male germline cells during meiosis and spermiogenesis: Comparison of young and aged testes in mice

et al. 2020

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Human epidemiological studies have shown that paternal aging as one of the risk factors for neurodevelopmental disorders, such as autism, in offspring. A recent study has suggested that factors other than de novo mutations due to aging can influence the biology of offspring. Here, we focused on epigenetic alterations in sperm that can influence developmental programs in offspring. In this study, we qualitatively and semiquantitatively evaluated histone modification patterns in male germline cells throughout spermatogenesis based on immunostaining of testes taken from young (3 months old) and aged (12 months old) mice. Although localization patterns were not obviously changed between young and aged testes, some histone modification showed differences in their intensity. Among histone modifications that repress gene expression, histone H3 lysine 9 trimethylation (H3K9me3) was decreased in the male germline cells of the aged testis, while H3K27me2/3 was increased. The intensity of H3K27 acetylation (ac), an active mark, was lower/higher depending on the stages in the aged testis. Interestingly, H3K27ac was detected on the putative sex chromosomes of round spermatids, while other chromosomes were occupied by a repressive mark, H3K27me3. Among other histone modifications that activate gene expression, H3K4me2 was drastically decreased in the male germline cells of the aged testis. In contrast, H3K79me3 was increased in M-phase spermatocytes, where it accumulates on the sex chromosomes. Therefore, aging induced alterations in the amount of histone modifications and in the differences of patterns for each modification. Moreover, histone modifications on the sex chromosomes and on other chromosomes seems to be differentially regulated by aging. These findings will help elucidate the epigenetic mechanisms underlying the influence of paternal aging on offspring development.

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Section: Morphological Profiles Showing Histone Modification Patternsmentioning

confidence: 99%

Comprehensive histochemical profiles of histone modification in male germline cells during meiosis and spermiogenesis: Comparison of young and aged testes in mice

et al. 2020

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“…Furthermore, porcine SSCs grown in the 0.2% (w/v) agarose‐based 3D hydrogels also showed the strongest transcriptional up‐regulation of OCT4 and THY1 and translational up‐regulation of NANOG and TRA‐1‐60. Moreover, significant transcriptional down‐regulation of C‐KIT , differentiation marker of spermatogonia (An et al, ), was detected in porcine SSCs cultured in 0.2% (w/v) agarose‐based 3D hydrogels compared with those in 2D microenvironments (Supporting Information Figure S3). Differentiation into spermatozoa still occurred in recipient mouse testis transplanted with undifferentiated porcine SSCs maintained in 0.2% (w/v) agarose‐based 3D hydrogels, as determined by the presence of spermatozoa with a round‐shaped head (Honaramooz et al, ) (Supporting Information Figure S4A) and a mitochondria containing pig‐specific mtDNA (Yoshida et al, ) (Supporting Information Figure S4B).…”

Section: Resultsmentioning

confidence: 99%

Porcine spermatogonial stem cells self‐renew effectively in a three dimensional culture microenvironment

Park

Kim

et al. 2017

Cell Biology International

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Generally, self-renewal of spermatogonial stem cells (SSCs) is maintained in vivo in a three-dimensional (3D) microenvironment consisting of the seminiferous tubule basement membrane, indicating the importance of the 3D microenvironment for in vitro culture of SSCs. Here, we report a 3D culture microenvironment that effectively maintains porcine SSC self-renewal during culture. Porcine SSCs were cultured in an agarose-based 3D hydrogel and in 2D culture plates either with or without feeder cells. Subsequently, the effects of 3D culture on the maintenance of undifferentiated SSCs were identified by analyzing cell colony formation and morphology, AP activity, and transcriptional and translational regulation of self-renewal-related genes and the effects on proliferation by analyzing cell viability and single cell-derived colony number. The 3D culture microenvironment constructed using a 0.2% (w/v) agarose-based 3D hydrogel showed the strongest maintenance of porcine SSC self-renewal and induced significant improvements in proliferation compared with 2D culture microenvironments. These results demonstrate that self-renewal of porcine SSCs can be maintained more effectively in a 3D than in a 2D culture microenvironment. Moreover, this will play a significant role in developing novel culture systems for SSCs derived from diverse species in the future, which will contribute to SSC-related research.

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“…Compared with yaks, the level of H3K27me3 was no significant difference in cattle-yaks testes. Research has shown that H3K27me3 is abundant in spermatogonial cells [12]. However, there were few spermatogonium cells in cattle-yak testicles, suggesting that the lack of difference in H3K27me3 between yaks and cattle-yaks is probably because of the antagonistic effect of H3K36me3 on H3K27me3 [12].…”

Section: Discussionmentioning

confidence: 99%

“…Epigenetics is characterized by RNA interference, chromatin remodeling, DNA methylation, and histone modifications, which are important regulators in a number of biological processes [11]. Epigenetics also plays important roles in spermatogenesis and meiosis, and histone modifications are involved in these processes [12][13][14]. Histones are chromosomal proteins and serve as the structural unit for packaging of DNA.…”

Section: Introductionmentioning

confidence: 99%

“…Methylation is an important form of histone modification and has been shown to be essential for spermatogenesis, including both addition and removal of methylation by some methyltransferases and demethylases, respectively [14,19]. Research showed that H3K4me2/3, H3K27me3, and H4K20ME1/2/3 are dynamically distributed throughout the various stages of spermatogenesis [12]. G9a is a methyltransferase that monomethylates and dimethylates H3K9, which then mediates epigenetic gene silencing and is pivotal for meiotic prophase progression [20].…”

Section: Introductionmentioning

confidence: 99%

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Cloning and Expression Analysis of Two Kdm Lysine Demethylases in the Testes of Mature Yaks and Their Sterile Hybrids

Shen

Huang

Jin

et al. 2020

Animals

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The objective of this study was to explore the molecular mechanism for male sterility of yak hybrids based on two demethylases. Total RNA was extracted from the testes of adult yaks (n = 10) and yak hybrids (cattle–yaks, n = 10). The coding sequences (CDS) of two lysine demethylases (KDMs), KDM1A and KDM4B, were cloned by RT-PCR. The levels of KDM1A and KDM4B in yaks and cattle–yaks testes were detected using Real-time PCR and Western blotting for mRNA and protein, respectively. In addition, the histone methylation modifications of H3K36me3 and H3K27me3 were compared between testes of yaks and cattle–yaks using ELISA. The CDS of KDM1A and KDM4B were obtained from yak testes. The results showed that the CDS of KDM1A exhibited two variants: variant 1 has a CDS of 2622 bp, encoding 873 amino acids, while variant 2 has a CDS of 2562 bp, encoding 853 amino acids. The CDS of the KDM4B gene was 3351 bp in length, encoding 1116 amino acids. The mRNA and protein expression of KDM1A and KDM4B, as well as the level of H3K36me3, were dramatically decreased in the testes of cattle–yaks compared with yaks. The present results suggest that the male sterility of cattle–yaks might be associated with reduced histone methylation modifications.

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Histone lysine methylation exhibits a distinct distribution during spermatogenesis in pigs

Cited by 14 publications

References 28 publications

Comprehensive histochemical profiles of histone modification in male germline cells during meiosis and spermiogenesis: Comparison of young and aged testes in mice

Comprehensive histochemical profiles of histone modification in male germline cells during meiosis and spermiogenesis: Comparison of young and aged testes in mice

Porcine spermatogonial stem cells self‐renew effectively in a three dimensional culture microenvironment

Cloning and Expression Analysis of Two Kdm Lysine Demethylases in the Testes of Mature Yaks and Their Sterile Hybrids

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