Histone-like protein H-NS regulates biofilm formation and virulence of Actinobacillus pleuropneumoniae

Dalai, Baolige; Zhou, Rui; Wan, Yun; Kang, Mingsong; Li, Lü; Li, Tingting; Zhang, Sihua; Chen, Huanchun

doi:10.1016/j.micpath.2008.11.005

Cited by 17 publications

(20 citation statements)

References 35 publications

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“…In this study, we have mapped the transposon insertion sites in these 61 mutants and found that all are located in one of two Tn10 hot spots within the gene encoding a homologue of H-NS, a DNA-binding protein involved in regulating the structure and function of the bacterial chromosome (19)(20)(21). A recent report indicated that mutation of hns in A. pleuropneumoniae leads to increased biofilm formation and that providing the cloned hns gene in trans restores the nonadherent phenotype (17). However, Dalai et al (17) did not investigate the genes involved in biofilm formation but rather went on to show that mutation of hns resulted in decreased expression of apxIIA and attenuation of virulence.…”

Section: Discussionmentioning

confidence: 98%

“…A recent report indicated that mutation of hns in A. pleuropneumoniae leads to increased biofilm formation and that providing the cloned hns gene in trans restores the nonadherent phenotype (17). However, Dalai et al (17) did not investigate the genes involved in biofilm formation but rather went on to show that mutation of hns resulted in decreased expression of apxIIA and attenuation of virulence. We also found increased biofilm formation by our hns mutants.…”

Section: Discussionmentioning

confidence: 99%

“…It is not clear why there is a discrepancy in expression levels of apxIIA, but it may be due to differences in medium components and growth conditions. In the Dalai et al (17) study, virulence was assessed as a 50% lethal dose (LD 50 ) following intraperitoneal injection in mice. The mouse is not the natural host for this bacterium nor is the peritoneum the natural site of infection.…”

Section: Discussionmentioning

confidence: 99%

“…In Salmonella and E. coli, H-NS is known to regulate hundreds of genes (36,39,42), whereas in other species, the number of regulated genes may be more restricted (58). Recently, Dalai et al (17) reported that H-NS regulates biofilm formation in A. pleuropneumoniae, but they did not determine the mechanism of regulation. In this paper, we investigate the role of H-NS and E in regulation of biofilm development in A. pleuropneumoniae.…”

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confidence: 99%

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Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ ^E and H-NS

Bossé

Sinha

et al. 2010

J Bacteriol

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Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-␤-1,6-N-acetyl-D-glucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form biofilms on the surfaces of polystyrene plates, is lost following serial passage in broth culture, and repeated passage of the nonadherent variants on solid media does not result in reversion to the adherent colony phenotype. In order to investigate the regulation of PGA expression and biofilm formation in A. pleuropneumoniae, we screened a bank of transposon mutants of the nonadherent serovar 1 strain S4074T and identified mutations in two genes, rseA and hns, which resulted in the formation of the adherent colony phenotype. In other bacteria, including the Enterobacteriaceae, H-NS acts as a global gene regulator, and RseA is a negative regulator of the extracytoplasmic stress response sigma factor E . Transcription profiling of A. pleuropneumoniae rseA and hns mutants revealed that both E and H-NS independently regulate expression of the pga operon. Transcription of the pga operon is initiated from a E promoter site in the absence of H-NS, and upregulation of E is sufficient to displace H-NS, allowing transcription to proceed. In A. pleuropneumoniae, H-NS does not act as a global gene regulator but rather specifically regulates biofilm formation via repression of the pga operon. Positive regulation of the pga operon by E indicates that biofilm formation is part of the extracytoplasmic stress response in A. pleuropneumoniae.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ ^E and H-NS

Bossé

Sinha

et al. 2010

J Bacteriol

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“…chrysanthemi, Sal. typhimurium and Actinobacillus pleuropneumoniae have been shown to be involved in virulence (Müller et al, 2006;Hildebrand et al, 2006;Nasser et al, 2001;Harrison et al, 1994;Dalai et al, 2009). Here, we present genetic evidence to demonstrate that the xrvA gene of Xoo plays an important role in virulence and regulates the expression of a number of virulence genes.…”

Section: Introductionmentioning

confidence: 51%

The xrvA gene of Xanthomonas oryzae pv. oryzae, encoding an H-NS-like protein, regulates virulence in rice

et al. 2009

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Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight disease in rice, one of the most serious rice diseases. The xrvA gene from Xoo strain 13751 encodes a protein containing a histone-like nucleoid-structuring protein (H-NS) domain. The expression of xrvA in strain 13751 was enhanced in XOM2 minimal medium. Mutation of the xrvA gene of strain 13751 led to a significant reduction in virulence in the host plant rice, a delayed hypersensitive response in the nonhost castor-oil plant, a decrease in extracellular polysaccharide and diffusible signal factor production, and an increase in intracellular glycogen accumulation. Northern hybridization analyses revealed that the virulence-associated genes hrpG, hrpX, rpfC, rpfF, rpfG and gumB were downregulated in the xrvA mutant compared to the wild-type and complemented strains. Interestingly, increase of copy number of xrvA in the wild-type strain 13751 resulted in a strain showing similar phenotypes as the xrvA mutant and a reduction of the expression of gumB, hrpX, rpfC, rpfF and rpfG. These findings indicate that the xrvA gene, which is highly conserved in the sequenced strains of Xanthomonas, encodes an important regulatory factor for the virulence of Xoo.

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Actinobacillus

MacInnes

2010

Pathogenesis of Bacterial Infections in Animals

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Histone-like protein H-NS regulates biofilm formation and virulence of Actinobacillus pleuropneumoniae

Cited by 17 publications

References 35 publications

Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ ^E and H-NS

Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ ^E and H-NS

The xrvA gene of Xanthomonas oryzae pv. oryzae, encoding an H-NS-like protein, regulates virulence in rice

Actinobacillus

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Histone-like protein H-NS regulates biofilm formation and virulence of Actinobacillus pleuropneumoniae

Cited by 17 publications

References 35 publications

Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ E and H-NS

Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ E and H-NS

The xrvA gene of Xanthomonas oryzae pv. oryzae, encoding an H-NS-like protein, regulates virulence in rice

Actinobacillus

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Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ ^E and H-NS

Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ ^E and H-NS