Histone H3 Dynamics Reveal Domains with Distinct Proliferation Potential in the Arabidopsis Root

Otero, Sofía; Desvoyes, Bénédicte; Peiró, Ramón; Gutiérrez, Crisanto

doi:10.1105/tpc.15.01003

Cited by 77 publications

(102 citation statements)

References 48 publications

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“…Furthermore, ongoing DNA synthesis in cotyledons has been shown by incorporation in of the nucleotide analog 5‐ethynyl‐2′‐deoxyuridine (EdU) 7‐d‐old seedlings (Jiang & Berger, ), together suggesting that DNA‐synthesis‐dependent deposition can explain the observed increased H3.1 occupancy at repetitive sequences at 5 dag. To explain the increase in H3.1 occupancy specifically at heterochromatin, we can envisage that after a genome‐wide incorporation during S‐phase, H3.1 is exchanged to H3.3 either in a transcription‐dependent manner at the genic regions analyzed here or globally in euchromatin as described previously during the transition from cell proliferation to differentiation in the root (Otero et al ., ). Interestingly, most H3.1‐encoding genes are not expressed in the dry seed (Kawakatsu et al ., ; Narsai et al ., ), in agreement with absence of replicative activity (Barroco et al ., ) pointing towards a peculiar H3.1 : H3.3 balance in the dry seed that might favor chromatin decondensation during germination.…”

Section: Discussionmentioning

confidence: 97%

“…Chromatin assembly mediated by CAF-1 is required for CC formation H3.1 is deposited by the CAF-1 complex that operates in a DNA-synthesis dependent manner (Otero et al, 2016;Jiang & Berger, 2017), whereas the HIR complex is responsible for assembly of the replacement variant H3.3 Duc et al, 2015). FASCIATA 1 (FAS1), FASCIATA 2 (FAS2) and HISTONE REGULATOR A (HIRA), encoding two subunits of the Arabidopsis CAF-1 complex, and the central subunit of the HIR complex, respectively, are expressed during early postgermination development in cotyledons (Fig.…”

Section: H31 Occupancy Increases At Repetitive Sequences During CC Fmentioning

confidence: 99%

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Replication‐coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development

et al. 2018

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Developmental phase transitions are often characterized by changes in the chromatin landscape and heterochromatin reorganization. In Arabidopsis, clustering of repetitive heterochromatic loci into so-called chromocenters is an important determinant of chromosome organization in nuclear space. Here, we investigated the molecular mechanisms involved in chromocenter formation during the switch from a heterotrophic to a photosynthetically competent state during early seedling development. We characterized the spatial organization and chromatin features at centromeric and pericentromeric repeats and identified mutant contexts with impaired chromocenter formation. We find that clustering of repetitive DNA loci into chromocenters takes place in a precise temporal window and results in reinforced transcriptional repression. Although repetitive sequences are enriched in H3K9me2 and linker histone H1 before repeat clustering, chromocenter formation involves increasing enrichment in H3.1 as well as H2A.W histone variants, hallmarks of heterochromatin. These processes are severely affected in mutants impaired in replication-coupled histone assembly mediated by CHROMATIN ASSEMBLY FACTOR 1 (CAF-1). We further reveal that histone deposition by CAF-1 is required for efficient H3K9me2 enrichment at repetitive sequences during chromocenter formation. Taken together, we show that chromocenter assembly during post-germination development requires dynamic changes in nucleosome composition and histone post-translational modifications orchestrated by the replication-coupled H3.1 deposition machinery.

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Section: Discussionmentioning

confidence: 97%

Section: H31 Occupancy Increases At Repetitive Sequences During CC Fmentioning

confidence: 99%

Replication‐coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development

et al. 2018

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“…We used live imaging to study CDT1a dynamics during the cell cycle in the growing Arabidopsis root meristem cells by following the CDT1a-CFP signal in plants also expressing the constitutively expressed HTR5 histone H3.3-mRFP (Otero et al, 2016). Since mitotic cells did not contain CDT1a-CFP we started to follow the progression of mitotic cells into the next cell cycle.…”

Section: Cdt1a Protein Is Loaded In Early G1 and Degraded At The G1/smentioning

confidence: 99%

FBL17 targets CDT1a for degradation in early S-phase to prevent Arabidopsis genome instability

Desvoyes

Noir

Masoud

et al. 2019

Preprint

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Maintenance of genome integrity depends on controlling the availability of DNA replication initiation proteins, e.g., CDT1, a component of the pre-replication complexes that regulates chromatin licensing for replication. To understand the evolutionary history of CDT1 regulation, we have identified the mechanisms involved in CDT1 dynamics. During cell cycle, CDT1a starts to be loaded early after mitotic exit and maintains high levels until the G1/S transition. Soon after the S-phase onset, CDT1a is rapidly degraded in a proteasomedependent manner. Plant cells use a specific SCF-mediated pathway that relies on the FBL17 F-box protein for CDT1a degradation, which is independent of CUL4a-containing complexes. A similar oscillatory pattern occurs in endoreplicating cells, where CDT1a is loaded just after finishing the S-phase. CDT1a is necessary to maintain genome stability, an ancient strategy although unique proteins and mechanisms have evolved in different eukaryotic lineages to ensure its degradation during S-phase.

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“…Nuestro grupo ha estudiado la dinámica de incorporación de histona H3 durante el desarrollo de órganos [6] y ha podido identificar una población celular única que se encuentra desarrollando su último ciclo celular antes de iniciar la diferenciación [7].…”

Section: Summary Organogenesis Requires a Strict Coordination Betweenunclassified

Dinámica de la cromatina y proliferación celular durante el desarrollo

Gutiérrez¹

2017

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Histone H3 Dynamics Reveal Domains with Distinct Proliferation Potential in the Arabidopsis Root

Cited by 77 publications

References 48 publications

Replication‐coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development

Replication‐coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development

FBL17 targets CDT1a for degradation in early S-phase to prevent Arabidopsis genome instability

Dinámica de la cromatina y proliferación celular durante el desarrollo

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