Histone H2B ubiquitylation: Connections to transcription and effects on chromatin structure

Fetian, Tasniem; Grover, Aakash; Arndt, Karen M.

doi:10.1016/j.bbagrm.2024.195018

Cited by 1 publication

(2 citation statements)

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“…H2Bub levels dropped considerably upon depletion of Paf1, Ctr9, and Rtf1. This observation suggests that, in the absence of ongoing H2B ubiquitylation via interactions between Paf1C and the H2B ubiquitylation machinery, 14 the kinetics of deubiquitylation are sufficiently swift to remove pre-existing H2Bub. The strongest effect on H2Bub levels was caused by Rtf1 depletion, which was expected given the direct role of the Rtf1 histone modification domain in stimulating H2B ubiquitylation through its interaction with Rad6.…”

Section: Resultsmentioning

confidence: 96%

“…13 Rtf1 is critical for the co-transcriptional mono-ubiquitylation of H2B (H2Bub) on K123 in S. cerevisiae (K120 in humans) and other histone post-translational modifications (PTMs), which require H2Bub for their deposition, H3K4me2/3 and H3K79me2/3. 14 In stimulating H2Bub, Rtf1 interacts directly with Rad6, the ubiquitin conjugase for H2B. 15,16 Rtf1 also governs the occupancy of a chromatin remodeler, Chd1, on gene bodies in yeast and stimulates elongation rate after RNAPII pause release in metazoans.…”

Section: Introductionmentioning

confidence: 99%

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Multiple direct and indirect roles of Paf1C in elongation, splicing, and histone post-translational modifications

Francette,

Arndt

2024

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Paf1C is a highly conserved protein complex with critical functions during eukaryotic transcription. Previous studies have shown that Paf1C is multi-functional, controlling specific aspects of transcription, ranging from RNAPII processivity to histone modifications. However, it is unclear how specific Paf1C subunits directly impact transcription and coupled processes. We have compared conditional depletion to steady-state deletion for each Paf1C subunit to determine the direct and indirect contributions to gene expression in Saccharomyces cerevisiae. Using nascent transcript sequencing, RNAPII profiling, and modeling of transcription elongation dynamics, we have demonstrated direct effects of Paf1C subunits on RNAPII processivity and elongation rate and indirect effects on transcript splicing and repression of antisense transcripts. Further, our results suggest that the direct transcriptional effects of Paf1C cannot be readily assigned to any particular histone modification. This work comprehensively analyzes both the immediate and extended roles of each Paf1C subunit in transcription elongation and transcript regulation.

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Section: Resultsmentioning

confidence: 96%