Histone H1 Is Required for Proper Regulation of Pyruvate Decarboxylase Gene Expression in
            <i>Neurospora</i>
            <i>crassa</i>

Folco, H. Diego; Freitag, Michael; Ramón, Ana; Temporini, Esteban D.; Alvarez, María Elena; García, Irene; Scazzocchio, Claudio; Selker, Eric U.; Rosa, Alberto L.

doi:10.1128/ec.2.2.341-350.2003

Cited by 38 publications

(53 citation statements)

References 74 publications

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“…Previous studies have shown that this GFP fusion allows clear visualization of ascospores, due to the strong fluorescence of the H1-GFP protein in their nuclei (27). Microscopic analysis of strains expressing the H1-GFP fusion protein confirmed the morphological differences observed in wild-type gpr-1 ϩ (N2276) and ⌬gpr-1 mutant (H1GFP) strains with regard to missing ostioles and ruptures in the perithecial wall (Fig.…”

supporting

confidence: 51%

“…Vector pMF280 contains the N. crassa histone H1 protein as a carboxyterminal GFP fusion, under the control of the ccg-1 promoter (27). Strain N2276 contains this vector targeted to the his-3 locus in an otherwise wild-type background (27).…”

Section: Methodsmentioning

confidence: 99%

“…Strain N2276 contains this vector targeted to the his-3 locus in an otherwise wild-type background (27). pMF280 was also electroporated into the ⌬gpr-1 his-3 strain 28-6-1 (Table 1).…”

Section: Methodsmentioning

confidence: 99%

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The Predicted G-Protein-Coupled Receptor GPR-1 Is Required for Female Sexual Development inthe Multicellular Fungus Neurospora crassa

Kryštofová

Borkovich

2006

Eukaryot Cell

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G-protein-coupled receptors (GPCRs) control important aspects of asexual and sexual development in eukaryotic organisms. We have identified a predicted GPCR in the filamentous fungus Neurospora crassa with similarity to cyclic AMP-receptor like GPCRs from Dictyostelium discoideum and GCR1 from Arabidopsis thaliana. Expression of gpr-1 is highest in female reproductive structures, and deletion of gpr-1 leads to defects during sexual development. Unfertilized female structures (protoperithecia) from ⌬gpr-1 strains are weakly pigmented, small, and submerged in the agar. The perithecia produced after fertilization have deformed beaks that lack ostioles, the openings through which ascospores are discharged. Localization studies using a GPR-1-green fluorescent protein fusion protein showed that GPR-1 is targeted to female reproductive structures. Genetic epistasis experiments with the three G␣ genes were inconclusive due to the early block in mating exhibited by ⌬gna-1 strains. Phenotypic analysis of mutants from a high-throughput N. crassa knockout project allowed identification of BEK-1, a homeodomain transcription factor that is a potential target of GPR-1. The perithecial defects of ⌬bek-1 strains are similar to those of the ⌬gpr-1 strain, and epistasis analysis indicates that bek-1 could function downstream of gpr-1 during postfertilization events. The effect must be posttranscriptional, as bek-1 transcript levels are not affected in ⌬gpr-1 strains. The lack of ostioles in ⌬gpr-1 and ⌬bek-1 mutants has an undesirable effect on the ability to spread progeny (ascospores) by the normal ejection mechanism and would severely compromise the fitness of these strains in nature.

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supporting

confidence: 51%

Section: Methodsmentioning

confidence: 99%

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The Predicted G-Protein-Coupled Receptor GPR-1 Is Required for Female Sexual Development inthe Multicellular Fungus Neurospora crassa

Kryštofová

Borkovich

2006

Eukaryot Cell

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“…Similar to other fungi (403), Neurospora has only one histone H1 gene (Table 4) (242). Studies with Neurospora, S. cerevisiae, Tetrahymena, Aspergillus nidulans, and Ascobolus immersus revealed that H1 is not essential in any of these organisms (44,252,640,729,817) but, rather, is implicated in the regulation of nitrogen and carbon metabolism (242,329,727).…”

Section: Chromatin Structure and Gene Regulationmentioning

confidence: 99%

Lessons from the Genome Sequence ofNeurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

Borkovich

Alex

Yarden

et al. 2004

Microbiol Mol Biol Rev

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595

618

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We present an analysis of over 1,100 of the ∼10,000 predicted proteins encoded by the genome sequence of the filamentous fungus Neurospora crassa. Seven major areas of Neurospora genomics and biology are covered. First, the basic features of the genome, including the automated assembly, gene calls, and global gene analyses are summarized. The second section covers components of the centromere and kinetochore complexes, chromatin assembly and modification, and transcription and translation initiation factors. The third area discusses genome defense mechanisms, including repeat induced point mutation, quelling and meiotic silencing, and DNA repair and recombination. In the fourth section, topics relevant to metabolism and transport include extracellular digestion; membrane transporters; aspects of carbon, sulfur, nitrogen, and lipid metabolism; the mitochondrion and energy metabolism; the proteasome; and protein glycosylation, secretion, and endocytosis. Environmental sensing is the focus of the fifth section with a treatment of two-component systems; GTP-binding proteins; mitogen-activated protein, p21-activated, and germinal center kinases; calcium signaling; protein phosphatases; photobiology; circadian rhythms; and heat shock and stress responses. The sixth area of analysis is growth and development; it encompasses cell wall synthesis, proteins important for hyphal polarity, cytoskeletal components, the cyclin/cyclin-dependent kinase machinery, macroconidiation, meiosis, and the sexual cycle. The seventh section covers topics relevant to animal and plant pathogenesis and human disease. The results demonstrate that a large proportion of Neurospora genes do not have homologues in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. The group of unshared genes includes potential new targets for antifungals as well as loci implicated in human and plant physiology and disease

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“…The his-3 targeting vector pMF272 has been used for overexpression of green fluorescent protein (GFP) fusion proteins under the control of the N. crassa ccg-1 promoter (Folco et al 2003). For overexpression of PRE-2-GFP, the entire pre-2 ORF was amplified from the G18A5 cosmid in PCRs using primers 13 and 14.…”

Section: Northern Analysismentioning

confidence: 99%

Roles for Receptors, Pheromones, G Proteins, and Mating Type Genes During Sexual Reproduction in Neurospora crassa

Kim

Wright²,

Park³

et al. 2012

Genetics

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Here we characterize the relationship between the PRE-2 pheromone receptor and its ligand, CCG-4, and the general requirements for receptors, pheromones, G proteins, and mating type genes during fusion of opposite mating-type cells and sexual sporulation in the multicellular fungus Neurospora crassa. PRE-2 is highly expressed in mat a cells and is localized in male and female reproductive structures. Dpre-2 mat a females do not respond chemotropically to mat A males (conidia) or form mature fruiting bodies (perithecia) or meiotic progeny (ascospores). Strains with swapped identity due to heterologous expression of pre-2 or ccg-4 behave normally in crosses with opposite mating-type strains. Coexpression of pre-2 and ccg-4 in the mat A background leads to self-attraction and development of barren perithecia without ascospores. Further perithecial development is achieved by inactivation of Sad-1, a gene required for meiotic gene silencing. Findings from studies involving forced heterokaryons of opposite mating-type strains show that presence of one receptor and its compatible pheromone is necessary and sufficient for perithecial development and ascospore production. Taken together, the results demonstrate that although receptors and pheromones control sexual identity, the mating-type genes (mat A and mat a) must be in two different nuclei to allow meiosis and sexual sporulation to occur.

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Histone H1 Is Required for Proper Regulation of Pyruvate Decarboxylase Gene Expression in Neurospora crassa

Cited by 38 publications

References 74 publications

The Predicted G-Protein-Coupled Receptor GPR-1 Is Required for Female Sexual Development inthe Multicellular Fungus Neurospora crassa

The Predicted G-Protein-Coupled Receptor GPR-1 Is Required for Female Sexual Development inthe Multicellular Fungus Neurospora crassa

Lessons from the Genome Sequence ofNeurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

Roles for Receptors, Pheromones, G Proteins, and Mating Type Genes During Sexual Reproduction in Neurospora crassa

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