Histone Genes in Trypanosomatids

Galanti, Norbel; Galindo, Mario; Sabaj, Valeria; Espinoza, Ingrid; Toro, G. Cecilia

doi:10.1016/s0169-4758(97)01162-9

Cited by 58 publications

(45 citation statements)

References 42 publications

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“…Indeed, at least five of the T cell-stimulating Leishmania Ags reported herein (␣-and ␤-tubulin, histone H2b, elongation factor 2a, and ribosomal protein S4) represent cytoplasmic or nuclear proteins that are abundantly expressed in the promastigote as well as amastigote life stage of the parasite (31,32). Furthermore, ␣-and ␤-tubulin are also major components of the leishmanial flagella.…”

Section: Discussionmentioning

confidence: 93%

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Probst

Stromberg

Ghalib

et al. 2001

The Journal of Immunology

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Persistent immunity against Leishmania infections in humans is mediated predominantly by CD4+ T cells of the Th1 phenotype. Herein we report the expression cloning of eight Leishmania Ags using parasite-specific T cell lines derived from an immune donor. The Ags identified by this technique include the flagellar proteins α- and β-tubulin, histone H2b, ribosomal protein S4, malate dehydrogenase, and elongation factor 2, as well as two novel parasite proteins. None of these proteins have been previously reported as T cell-stimulating Ags from Leishmania. β-tubulin-specific T cell clones generated against Leishmania major amastigotes responded to Leishmania-infected macrophages and dendritic cells. IFN-γ enzyme-linked immunospot analysis demonstrated the presence of T cells specific for several of these Ags in PBMC from self-healing cutaneous leishmaniasis patients infected with either Leishmania tropica or L. major. The responses elicited by Leishmania histone H2b were particularly striking in terms of frequency of histone-specific T cells in PBMC (1 T cell of 6000 PBMC) as well as the percentage of responding donors (86%, 6 of 7). Ags identified by T cells from immune donors might constitute potential vaccine candidates for leishmaniasis.

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Section: Discussionmentioning

confidence: 93%

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Probst

Stromberg

Ghalib

et al. 2001

The Journal of Immunology

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“…Despite the fact that histones are among the most highly conserved protein along the evolutionary scale, histones of trypanosomatids have accumulated substantial sequence differences, mainly at the N-and C-terminal region. 16 In fact, the specificity of the humoral response ellicited against parasite histones during Leishmania infection is because of the localization of the main antigenic determinants in those divergent regions. 17 This helped to develop a recombinant multiepitope protein containing the antigenic determinants of the H2A histone that represent a valuable tool for the serodiagnosis of canine visceral leishmaniosis.…”

mentioning

confidence: 99%

A DNA aptamer population specifically detects Leishmania infantum H2A antigen

Ramos¹,

Piñeiro²,

Soto

et al. 2007

Laboratory Investigation

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“…Trypanosoma cruzi core histones presented divergent amino acid sequences and dissimilar electrophoretic mobilities when compared to higher eukaryotes [3][4][5]8]. These characteristics of T. cruzi core histones are quite likely related to the failure of its chromatin to condense into chromosomes [27].…”

Section: Discussionmentioning

confidence: 99%

“…However, these chromosomal proteins present a high degree of divergence in lower eukaryotes, such as Entamoeba [1], Trichomonas [2], Trypanosoma [3][4][5][6][7][8], Leishmania and Crithidia [9], dinoflagellates [10], ciliata [11] and Giardia [12]. Histone divergence during evolution is expressed at the amino acid sequence level in core histones and mostly at the structural level in H1 histones.…”

Section: Introductionmentioning

confidence: 99%

Chromatin from two classes of platyhelminthes display both protist H1 and higher eukaryote core histones

et al. 2004

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Histones from the parasitic platyhelminthes, Echinococcus granulosus and Fasciola hepatica, were systematically characterized. Core histones H2A, H2B, H3 and H4, which were identified on the basis of amino acid sequencing and mass spectrometry data, showed conserved electrophoretic patterns. Histones H1, identified on the basis of physicochemical properties, amino acid composition and amino acid sequencing, showed divergence, both in their number and electrophoretic mobilities, between the two species and among other organisms. According to these data, core histones but not H1 histones, would be stabilized during evolution at the level of platyhelminthes.

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Histone Genes in Trypanosomatids

Cited by 58 publications

References 42 publications

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

A DNA aptamer population specifically detects Leishmania infantum H2A antigen

Chromatin from two classes of platyhelminthes display both protist H1 and higher eukaryote core histones

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