Histone Deposition Proteins: Links between the DNA Replication Machinery and Epigenetic Gene Silencing

Franco, Alexa A.; Kaufman, Paul D.

doi:10.1101/sqb.2004.69.17

Cited by 4 publications

(4 citation statements)

References 89 publications

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“…If there is a blockage in the spreading of heterochromatic marks from the RD29A promoter to the 35S promoter, then already established H3K9 methylation at 35S would need to be maintained following cell division, in order to effectively repress the NPTII gene. During DNA replication, there is a redistribution of histones, resulting in a dilution of histone modifications in daughter chromatids [39]. To regain the level of histone modifications, the histones from the parent provide a molecular bookmark that enables the formation of new modifications in histones.…”

Section: Discussionmentioning

confidence: 99%

Mutations in a Conserved Replication Protein Suppress Transcriptional Gene Silencing in a DNA- Methylation-Independent Manner in Arabidopsis

et al. 2005

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Mutations in the DNA glycosylase/lyase ROS1 cause transcriptional silencing of the linked RD29A-LUC and 35S-NPTII transgenes in Arabidopsis. We report here that mutations in the Arabidopsis RPA2 locus release the silencing of 35S-NPTII but not RD29A-LUC in the ros1 mutant background. The rpa2 mutation also leads to enhanced expression of some transposons. Neither DNA methylation nor siRNAs at any of the reactivated loci are blocked by rpa2. Histone H3 methylation at lysine 4 was increased and histone H3 methylation at lysine 9 was decreased at the 35S promoter in the ros1rpa2 mutant compared to the ros1 background. RPA2 encodes a nuclear protein similar to the second subunit of the replication protein A conserved from yeast to mammals. Ectopic expression of the Arabidopsis RPA2 could complement the yeast rfa2 (rpa2) mutant. These results suggest an essential role of RPA2 in the maintenance of transcriptional gene silencing at specific loci in a DNA-methylation-independent manner. In addition, we found that rpa2 mutants are hypersensitive to the genotoxic agent methyl methanesulphonate, and the RPA2 protein interacts with ROS1 in vitro and in vivo, suggesting that RPA2 also functions together with ROS1 in DNA repair.

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Section: Discussionmentioning

confidence: 99%

Mutations in a Conserved Replication Protein Suppress Transcriptional Gene Silencing in a DNA- Methylation-Independent Manner in Arabidopsis

et al. 2005

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“…The first is simply that the presence behind replication forks of partially assembled nucleosome arrays and stretches of naked DNA somehow causes catastrophic DNA damage and/or chromosome segregation defects. The second model invokes the existence of a chromatin assembly surveillance mechanism that, in wild‐type cells, prevents the generation of long stretches of immature nucleosome arrays by slowing down or pausing replication forks [35]. This would provide time for the de novo nucleosome assembly pathway to catch up with the replisome in order to deposit histones immediately behind replication forks.…”

Section: The Two Faces Of Histone Chaperones: Nucleosome Assembly Andmentioning

confidence: 99%

Clothing up DNA for all seasons: Histone chaperones and nucleosome assembly pathways

Rocha

Verreault

2008

FEBS Letters

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In eukaryotes, the packaging of DNA into chromatin is essential for cell viability. Several important DNA metabolic events require the transient disruption of chromatin structure, but cells have evolved a number of elaborate pathways that operate throughout the cell cycle to prevent the deleterious effects of chromatin erosion. In this review, we describe a number of distinct nucleosome assembly pathways that function during DNA replication, transcription, cellular senescence and early embryogenesis. In addition, we illustrate some of the physiological consequences associated with defects in nucleosome assembly pathways.

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“…

The orderly deposition of histones onto DNA is mediated by conserved assembly complexes, including Chromatin Assembly Factor-1 (CAF-1) and the Hir proteins [1][2][3][4]. CAF-1 and the Hir proteins operate in distinct but functionally overlapping histone deposition pathways in vivo [5,6].

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confidence: 99%

“…Histone deposition by HIR and Asf1/H3/H4 complexes. (A) 30 fmol of a 250 bp radiolabeled DNA probe was incubated with 100 fmol of histones H3/H4 (lanes 2-4), 100 fmol of preformed Asf1/H3/H4 complex (lanes 5-9), 100 fmol of Asf1 (lanes 10-12) and either 1.75 μL (lane 6), 3.5 μL (lane 7), or 7 μL (lanes 3, 8, 11, 13) of HIR complex or HIR dialysis buffer (7 μL; lanes4,9,12,14). Reaction products were resolved on a native 4% polyacrylamide gel and detected by autoradiography.…”

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confidence: 99%

Replication-Independent Histone Deposition by the HIR Complex and Asf1

et al. 2005

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The orderly deposition of histones onto DNA is mediated by conserved assembly complexes, including chromatin assembly factor-1 (CAF-1) and the Hir proteins . CAF-1 and the Hir proteins operate in distinct but functionally overlapping histone deposition pathways in vivo . The Hir proteins and CAF-1 share a common partner, the highly conserved histone H3/H4 binding protein Asf1, which binds the middle subunit of CAF-1 as well as to Hir proteins . Asf1 binds to newly synthesized histones H3/H4 , and this complex stimulates histone deposition by CAF-1 . In yeast, Asf1 is required for the contribution of the Hir proteins to gene silencing . Here, we demonstrate that Hir1, Hir2, Hir3, and Hpc2 comprise the HIR complex, which copurifies with the histone deposition protein Asf1. Together, the HIR complex and Asf1 deposit histones onto DNA in a replication-independent manner. Histone deposition by the HIR complex and Asf1 is impaired by a mutation in Asf1 that inhibits HIR binding. These data indicate that the HIR complex and Asf1 proteins function together as a conserved eukaryotic pathway for histone replacement throughout the cell cycle.

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Histone Deposition Proteins: Links between the DNA Replication Machinery and Epigenetic Gene Silencing

Cited by 4 publications

References 89 publications

Mutations in a Conserved Replication Protein Suppress Transcriptional Gene Silencing in a DNA- Methylation-Independent Manner in Arabidopsis

Mutations in a Conserved Replication Protein Suppress Transcriptional Gene Silencing in a DNA- Methylation-Independent Manner in Arabidopsis

Clothing up DNA for all seasons: Histone chaperones and nucleosome assembly pathways

Replication-Independent Histone Deposition by the HIR Complex and Asf1

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