Histone Demethylases as Counterbalance to H3K27me3 Silencing in Plants

Crevillén, Pedro

doi:10.1016/j.isci.2020.101715

Cited by 25 publications

(18 citation statements)

References 61 publications

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“…UTX and JMJD3 are less conserved in plants, but up to five jumonji proteins have been identified as bona fide H3K27me3 demethylases in Arabidopsis, including EARLY FLOWER-ING 6 (ELF6/JMJ11), RELATIVE OF EARLY FLOWERING 6 (REF6/JMJ12), JMJ13, JMJ30, and JMJ32 [51]. They have largely non-overlapping chromatin-targeting machineries and biological functions.…”

Section: Writers and Erasers Of H3k27me3 In Plantsmentioning

confidence: 99%

Dynamics of H3K27me3 Modification on Plant Adaptation to Environmental Cues

Shen

Lin

et al. 2021

Plants

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Given their sessile nature, plants have evolved sophisticated regulatory networks to confer developmental plasticity for adaptation to fluctuating environments. Epigenetic codes, like tri-methylation of histone H3 on Lys27 (H3K27me3), are evidenced to account for this evolutionary benefit. Polycomb repressive complex 2 (PRC2) and PRC1 implement and maintain the H3K27me3-mediated gene repression in most eukaryotic cells. Plants take advantage of this epigenetic machinery to reprogram gene expression in development and environmental adaption. Recent studies have uncovered a number of new players involved in the establishment, erasure, and regulation of H3K27me3 mark in plants, particularly highlighting new roles in plants’ responses to environmental cues. Here, we review current knowledge on PRC2-H3K27me3 dynamics occurring during plant growth and development, including its writers, erasers, and readers, as well as targeting mechanisms, and summarize the emerging roles of H3K27me3 mark in plant adaptation to environmental stresses.

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Section: Writers and Erasers Of H3k27me3 In Plantsmentioning

confidence: 99%

Dynamics of H3K27me3 Modification on Plant Adaptation to Environmental Cues

Shen

Lin

et al. 2021

Plants

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“…In maize T-CMS, the restorer gene RF2 encodes an ALDH that can complement the lack of ALDH activity in its mitochondria to reverse the male sterility caused by the CMS protein URF13 ( Liu et al, 2001 ). ASH/SDG genes are negative regulators of H3K27me3 (which is known to downregulate genes involved in cellular fate maintenance during development in plants and animals) ( Cartagena et al, 2008 ; Lee et al, 2015 ; Crevillen, 2020 ; Cai et al, 2021 ). ASH/SDG is located upstream of ALDH in the lysine degradation pathway (ko00310).…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

Yang

Nong

et al. 2021

Front. Plant Sci.

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Crosses that lead to heterosis have been widely used in the rapeseed (Brassica napus L.) industry. Cytoplasmic male sterility (CMS)/restorer-of-fertility (Rf) systems represent one of the most useful tools for rapeseed production. Several CMS types and their restorer lines have been identified in rapeseed, but there are few studies on the mechanisms underlying fertility restoration. Here, we performed morphological observation, map-based cloning, and transcriptomic analysis of the F2 population developed by crossing the CMS line WNJ01A with its restorer line Hui01. Paraffin-embedded sections showed that the sporogenous cell stage was the critical pollen degeneration period, with major sporogenous cells displaying loose and irregular arrangement in sterile anthers. Most mitochondrial electron transport chain (mtETC) complex genes were upregulated in fertile compared to sterile buds. Using bulked segregant analysis (BSA)-seq to analyze mixed DNA pools from sterile and fertile F2 buds, respectively, we identified a 6.25 Mb candidate interval where Rfw is located. Using map-based cloning experiments combined with bacterial artificial chromosome (BAC) clone sequencing, the candidate interval was reduced to 99.75 kb and two pentatricopeptide repeat (PPR) genes were found among 28 predicted genes in this interval. Transcriptome sequencing showed that there were 1679 DEGs (1023 upregulated and 656 downregulated) in fertile compared to sterile F2 buds. The upregulated differentially expressed genes (DEGs) were enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) lysine degradation pathway and phenylalanine metabolism, and the downregulated DEGs were enriched in cutin, suberine, and wax biosynthesis. Furthermore, 44 DEGs were involved in pollen and anther development, such as tapetum, microspores, and pollen wall development. All of them were upregulated except a few such as POE1 genes (which encode Pollen Ole e I allergen and extensin family proteins). There were 261 specifically expressed DEGs (9 and 252 in sterile and fertile buds, respectively). Regarding the fertile bud-specific upregulated DEGs, the ubiquitin–proteasome pathway was enriched. The top four hub genes in the protein–protein interaction network (BnaA09g56400D, BnaA10g18210D, BnaA10g18220D, and BnaC09g41740D) encode RAD23d proteins, which deliver ubiquitinated substrates to the 26S proteasome. These findings provide evidence on the pathways regulated by Rfw and improve our understanding of fertility restoration.

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“…In addition, H3K9 methylation status at some loci can also be modulated by elf6 and ref6 loss-of-function mutations ( Yu et al, 2008 ), indicating a potential link between the erasure of H3K9 and H3K27 methylation. Two JmjC domain-only proteins, JMJ30 and JMJ32, demethylate H3K27me2/3 jointly at the FLC locus to regulate flowering at elevated temperatures ( Gan et al, 2014 ; Crevillen, 2020 ). JMJ30 can also act as an eraser to remove H3K36me2/3 ( Yan et al, 2014 ) and H3K9me3 marks ( Lee et al, 2018 ), suggesting multiple roles of a single demethylase in various lysine demethylation.…”

Section: “Writers” “Readers” and “Erasers” For Histone H3 Lysine Methylationmentioning

confidence: 99%

Reprogramming of Histone H3 Lysine Methylation During Plant Sexual Reproduction

Fang

Shao

2021

Front. Plant Sci.

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Plants undergo extensive reprogramming of chromatin status during sexual reproduction, a process vital to cell specification and pluri- or totipotency establishment. As a crucial way to regulate chromatin organization and transcriptional activity, histone modification can be reprogrammed during sporogenesis, gametogenesis, and embryogenesis in flowering plants. In this review, we first introduce enzymes required for writing, recognizing, and removing methylation marks on lysine residues in histone H3 tails, and describe their differential expression patterns in reproductive tissues, then we summarize their functions in the reprogramming of H3 lysine methylation and the corresponding chromatin re-organization during sexual reproduction in Arabidopsis, and finally we discuss the molecular significance of histone reprogramming in maintaining the pluri- or totipotency of gametes and the zygote, and in establishing novel cell fates throughout the plant life cycle. Despite rapid achievements in understanding the molecular mechanism and function of the reprogramming of chromatin status in plant development, the research in this area still remains a challenge. Technological breakthroughs in cell-specific epigenomic profiling in the future will ultimately provide a solution for this challenge.

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Histone Demethylases as Counterbalance to H3K27me3 Silencing in Plants

Cited by 25 publications

References 61 publications

Dynamics of H3K27me3 Modification on Plant Adaptation to Environmental Cues

Dynamics of H3K27me3 Modification on Plant Adaptation to Environmental Cues

Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

Reprogramming of Histone H3 Lysine Methylation During Plant Sexual Reproduction

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