Histone Deacetylases and SAP18, a Novel Polypeptide, Are Components of a Human Sin3 Complex

Zhang, Yi; Iratni, Rabah; Erdjument‐Bromage, Hediye; Tempst, Paul; Reinberg, Danny

doi:10.1016/s0092-8674(00)80216-0

Cited by 533 publications

(504 citation statements)

References 43 publications

Supporting

Mentioning

483

Contrasting

Order By: Relevance

“…reported activation by Myc (rather than repression) and the presence of a Myc binding site with regulatory region. a Bush et al, 1998; Boyd and Farnham, 1997;Boyd et al, 1998;c As noted in the text, Myc,Max dimers have been reported to bind an MrDb pseudogene in vivo Grandori et al, 1996;d Tavtigian et al, 1994;Tsuneoka et al, 1997; Grandori et al, 1996;f Tavtigian et al, 1994;Shim et al, 1997; Eilers et al, 1991;h Galaktionov et al, 1996;i Weihua et al, 1997;j Tsuneoka et al, 1997;k Reisman et al, 1993;Tavtigian et al, 1994;l Miltenberger et al, 1995; (Hassig et al, 1997;Laherty et al, 1997;Zhang et al, 1997;Sommer et al, 1997).…”

Section: Trrap Links Myc Transactivation To Chromatin Remodeling and mentioning

confidence: 95%

The Myc oncoprotein: a critical evaluation of transactivation and target gene regulation

1999

View full text Add to dashboard Cite

Section: Trrap Links Myc Transactivation To Chromatin Remodeling and mentioning

confidence: 95%

The Myc oncoprotein: a critical evaluation of transactivation and target gene regulation

1999

View full text Add to dashboard Cite

“…A cDNA fragment confirmed to represent T-plastin 24 was cloned from a liver cDNA library. 25 The IMAGE cDNA clone 201315 (GenBank accession number R99689), corresponding to Sin3-associated polypeptide 18 kDa (SAP18), 26 and a cDNA fragment (GenBank accession number X89832), corresponding to mitochondrial DNA for loop attachment sequence, 27 were purchased from the American Type Culture Collection (Manassas, VA). Finally, to evaluate the amounts of RNA loaded, the filters were hybridized to kinaselabeled oligonucleotides specific for human 18S rRNA or a conserved sequence of 28S rRNA.…”

Section: Differential Display Analysismentioning

confidence: 99%

Differential display analysis of breast carcinoma cells enriched by immunomagnetic target cell selection: Gene expression profiles in bone marrow target cells

Ree

Engebraaten

Hovig

et al. 2001

Intl Journal of Cancer

View full text Add to dashboard Cite

The red bone marrow (BM) is an important indicator organ of hematogenous micrometastatic spread of carcinomas. Characterization of biological properties specific for BM micrometastatic cells, however, is technically challenging due to the limited number of target cells usually available for the purpose. This report provides referrals to qualitative gene expression profiling of BM micrometastatic cells enriched by immunomagnetic selection. First, an experimental strategy was used to study regulatory mechanisms involved when BM micrometastatic cells colonize distant organs. The MA-11 cells, originating from BM micrometastases in a breast cancer patient clinically devoid of overt metastatic disease, were injected into immunodeficient rats. Metastatic MA-11 cells were subsequently immunoselected from the resulting in vivo lesions. The selected cell populations were compared to the injected cells by differential display analysis, and several genes possibly involved in tumor cell invasion and proliferation were confirmed as differentially expressed among the various MA-11 cell populations. A direct approach to qualitative gene expression profiling of BM micrometastatic cells was also explored. Carcinoma cells were immunoselected from BM and axillary lymph nodes obtained from breast cancer patients, and the isolated cell populations were compared by differential display analysis. Two candidate genes, identified as factors involved in cellular growth control, appeared as differentially expressed by the target cells from BM. Our study provides detailed information on how to combine an immunomagnetic selection procedure and differential display analysis to reveal gene expression profiles that may characterize BM micrometastatic cells. © 2002 Wiley-Liss, Inc. Key words: bone marrow; micrometastasis; breast cancer; immunomagnetic cell preparation; differential cloningClinical and experimental evidence suggests that epithelial tumor cells are able to disseminate to secondary organs at an early stage of primary tumor development. The red bone marrow (BM) compartment represents an important indicator organ of hematogenous micrometastatic spread of carcinomas. According to current concepts, however, disseminated tumor cells detected in the BM are not able to grow as distant metastatic lesions unless they possess certain biological characteristics, among which the ability of invasion into and proliferation within the target organ is considered highly significant. [1][2][3] The classical, experimental works on mechanisms of tumor metastasis demonstrated that only a small subset of cells within the parental population is capable of metastasizing 4 and that the cellular composition of secondary tumors differs from that of the primaries. 5 Subsequent reports introduced the concept of dynamic heterogeneity, which argues that although the metastatic phenotype is a genetically controlled trait, it is inherently unstable. 6 Indeed, cellular properties that are crucial for initiation of distant tumor growth may be obscured by the heterog...

show abstract

“…Indeed SMRT and N-CoR interact strongly with unliganded receptors and are released upon ligand binding. A series of recent reports (Alland et al, 1997;Hassig et al, 1997;Heinzel et al, 1997;Kadosh and Struhl, 1997;Laherty et al, 1997;Zhang et al, 1997) have shown that SMRT/ N-CoR co-repressors are part of a multiprotein complex including the Mad co-repressors mSin3A and B (Ayer et al, 1995;Schreiber-Agus et al, 1995) and the histone deacetylases HDAC1/HD1 and HDAC2/mRPD3 (Taunton et al, 1996;Yang et al, 1996). These studies, that strengthen the major role of histone acetylation/deacetylation in transcriptional regulation, provide the basis for the existence of a common repression pathway between nuclear receptors and basic region-helix ± loop ± helix-leucine zipper (bHLH-Zip) proteins.…”

Section: Introductionmentioning

confidence: 99%

Histone deacetylase associated with mSin3A mediates repression by the acute promyelocytic leukemia-associated PLZF protein

et al. 1998

View full text Add to dashboard Cite

The PLZF gene was identi®ed ®rst by its fusion with the retinoic acid receptor a gene in the t(11;17) translocation associated with a retinoic acid resistant form of acute promyelocytic leukemia (APL). It encodes a kruÈppel-like zinc ®nger protein with a POZ domain shared with a subset of regulatory proteins including the BCL6 leukemogenic protein. PLZF, like BCL6, strongly represses transcription initiated from di erent promoters. Here we show that PLZF associates in vitro and in vivo with the Mad co-repressor mSin3A and the histone deacetylase HDAC1. Two domains in PLZF and the PAH1 structure of mSin3A mediate these interactions. Trichostatin A, a speci®c inhibitor of histone deacetylases, signi®cantly reduces PLZF repression. These data strongly suggest that, like nuclear receptors and Mad, PLZF represses transcription by recruiting a histone deacetylase through the SMRT-mSin3-HDAC co-repressor complex. We also show that BCL6 associates with HDAC1 indicating that this type of regulation might be common to POZ/Zinc ®nger proteins involved in human leukemias. This work supports a role for deregulated histone deacetylation in the development of both lymphoid and myeloid neoplasia in human and suggests that targeted histone deacetylase inhibitors may be useful for treatment of certain types of malignancies.

show abstract

Histone Deacetylases and SAP18, a Novel Polypeptide, Are Components of a Human Sin3 Complex

Cited by 533 publications

References 43 publications

The Myc oncoprotein: a critical evaluation of transactivation and target gene regulation

The Myc oncoprotein: a critical evaluation of transactivation and target gene regulation

Differential display analysis of breast carcinoma cells enriched by immunomagnetic target cell selection: Gene expression profiles in bone marrow target cells

Histone deacetylase associated with mSin3A mediates repression by the acute promyelocytic leukemia-associated PLZF protein

Contact Info

Product

Resources

About