Histone Deacetylase Inhibitor (Trapoxin A) Enhances Stemness Properties in Adipose Tissue Derived Mesenchymal Stem Cells

Mousazadeh, Leila; Zarghami, Nosratollah; Hashemzadeh, Shahryar; Aval, Sedigheh Fekri; Hasanifard, Leili; Herizchi, Roya

doi:10.1055/s-0044-102007

Cited by 6 publications

(3 citation statements)

References 31 publications

(38 reference statements)

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“…Among these, FR901375 chromopeptide A, FR901375, largazole, spiruchostatin A, HC-toxin, trapoxin, and azumamide are currently investigated for their anti-tumorigenic potential [252]. Trapoxin in particular appears to have a high affinity with HDAC8 [260] and has been shown to be inducing stemness genes (such as NANOG, SOX2, and TERT) in adipose tissue derived mesenchymal cells in vitro [261].…”

Section: Group Iv-cyclic Peptidesmentioning

confidence: 99%

Histone Deacetylases (HDACs): Evolution, Specificity, Role in Transcriptional Complexes, and Pharmacological Actionability

Milazzo

Mercatelli

Muzio

et al. 2020

Genes

193

139

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Histone deacetylases (HDACs) are evolutionary conserved enzymes which operate by removing acetyl groups from histones and other protein regulatory factors, with functional consequences on chromatin remodeling and gene expression profiles. We provide here a review on the recent knowledge accrued on the zinc-dependent HDAC protein family across different species, tissues, and human pathologies, specifically focusing on the role of HDAC inhibitors as anti-cancer agents. We will investigate the chemical specificity of different HDACs and discuss their role in the human interactome as members of chromatin-binding and regulatory complexes.

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Section: Group Iv-cyclic Peptidesmentioning

confidence: 99%

Histone Deacetylases (HDACs): Evolution, Specificity, Role in Transcriptional Complexes, and Pharmacological Actionability

Milazzo

Mercatelli

Muzio

et al. 2020

Genes

193

139

View full text Add to dashboard Cite

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“…After about 24 to 48 hours, the cells were passaged. AMSCs were obtained as described elsewhere 19‐21 . AMSCs cells were cultured in DMEM (Biosera, Iran), improved with 10% FBS and treated with penicillin 100 IU/mL and streptomycin 100 μg/mL at a cell incubator 37°C and 5% CO 2 , until reaching 80% confluence.…”

Section: Methodsmentioning

confidence: 99%

The effect of exogenous ciliary neurotrophic factor on cell cycle and neural differentiation markers of in vitro model cells: New insights for future therapeutic approaches

Ghasemi

Motlagh

Zarghami

2021

Cell Biochemistry & Function

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Retinoblastoma is known as childhood rare malignancy of the retina. Ciliary neurotrophic factor (CNTF) was previously found to reduce degeneration and promote retina survival. This work investigated the effects of CNTF supplementation on in‐vitro model cells including retinoblastoma (Y79) and adipose‐derived mesenchymal stem cells (AMSCs) viability, proliferation, gene expression and cell cycle. A drop of viability was detected in Y79 treated with CNTF in a dose‐dependent manner (P < .05). However, the proliferation of AMSCs was increased at lower concentrations of CNTF (5 ng/mL), but declined in higher doses (50 and 100 ng/mL). The BrdU assay confirmed the MTT assay results. Cell cycle was arrested in both Y79 and AMSCs in the G0/G1 phase by CNTF treatment. A considerable down‐regulation of Bcl2, CycD1 and N‐Myc genes expression (P < .05) inversely, P15 and P21 genes up‐regulation in treated Y79 cells was observed. Besides, stemness genes' transcription was reduced in AMSCs (P < .05), and levels of neuronal‐specific markers such as neuron‐specific enolase (NSE) and neuronal nuclei (NeuN) were increased (P < .05). The findings of this study suggest a promising potential of CNTF in terms of arresting Y79 retinoblastoma cells, and differentiation‐inducing to AMSCs, which could be valuable for managing future innovative treatments targeting retinoblastoma. Significance of the study We demonstrate that CNTF has the potential to reduce proliferation of Y79 cells and induce the cell cycle arrest of them. Also, down‐regulation of oncogenes (such as N‐Myc) while up‐regulation of tumour suppressor genes (such as P21) was detected by exposure of Y79 cells to CNTF. Furthermore, we observed the cell cycle arrest, reduction of stemness gene and up‐regulation of neural differentiation markers in AMSCs treated with CNTF. These results support the probable promising effects of CNTF for controlling retinoblastoma.

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“…One method for the nuclear qualitative study of apoptosis is a fluorescent dye DAPI which binds to DNA and is detectable by appropriate microscopic. We used a protocol as previously reported for DAPI staining [68], shortly: the MCF-7 or MDA-MB-231 cells were plated in 6 well format vessels at a density of 5 ×10 5 and let them attach and grow for 24 hours. After treatment with MTX, Au@SiO 2 NPs and MTX-FA loaded Au@SiO 2 NPs with and without laser treatment the cells were washed with PBS (Sigma) and then subjected to fixation by 10% formaldehyde (Merck), next: cells were permeabilized with Triton X-100 (Sigma) for 15 minutes.…”

Section: Apoptosis Study By Fluorescence Microscopymentioning

confidence: 99%

Novel Chemo-Photothermal Therapy in Breast Cancer Using Methotrexate-Loaded Folic Acid Conjugated Au@SiO2 Nanoparticles

et al. 2020

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Low level laser therapy (LLLT) is known as a safe type of phototherapy to target tumor tissue/cells. Besides, using targeted nanoparticles increases the successfulness of cancer therapy. This study was designed for investigating the combined effect of folate (FA)/Methotrexate (MTX) loaded silica coated gold (Au@SiO 2) nanoparticles (NPs) and LLLT on the fight against breast cancer. NPs were synthesized and characterized using FTIR, TEM and DLS-Zeta. The NPs had spherical morphology with mean diameter of around 25 nm and positive charge (+13.3 mV) while after conjugation with FA and MTX their net charge reduced to around-19.7 mV. Our findings in cell uptake studies clearly showed enhanced cellular uptake of NPs after FA and MTX loaded NPs in both breast cancer cell lines especially on MDA-MB-231 due to high expression of folate receptors. The results indicated that LLLT had a proliferative effect on both breast cancer cell lines but in the presence of engineered breast cancer targeted nanoparticle, the efficacy of combination chemo-photothermal therapy was significantly increased using MTT assay (p<0.05), DAPI staining, and cell cycle findings. The highest apoptotic effect on breast cancer cell lines was observed in the cells exposed to a combination of MTX-FA loaded Au@SiO 2 NP and LLLT proved by DAPI staining and cell cycle(by increasing the cell arrest in subG0/G1). Taken together a combination of chemotherapy and LLLT improves the potential of breast cancer therapy with minimum side effects.

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Histone Deacetylase Inhibitor (Trapoxin A) Enhances Stemness Properties in Adipose Tissue Derived Mesenchymal Stem Cells

Abstract: The addition of low dose of TPX to the expansion medium could possibly enhance the stemness properties and prevent the quality decline of ex-vivo cultured ASCs.

Cited by 6 publications

References 31 publications

Histone Deacetylases (HDACs): Evolution, Specificity, Role in Transcriptional Complexes, and Pharmacological Actionability

Histone Deacetylases (HDACs): Evolution, Specificity, Role in Transcriptional Complexes, and Pharmacological Actionability

The effect of exogenous ciliary neurotrophic factor on cell cycle and neural differentiation markers of in vitro model cells: New insights for future therapeutic approaches

Novel Chemo-Photothermal Therapy in Breast Cancer Using Methotrexate-Loaded Folic Acid Conjugated Au@SiO2 Nanoparticles

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