Histone deacetylase inhibition-mediated neuronal differentiation via the Wnt signaling pathway in human adipose tissue-derived mesenchymal stem cells

Jang, Sujeong; Jeong, Han-Seong

doi:10.1016/j.neulet.2018.01.006

Cited by 29 publications

(40 citation statements)

References 38 publications

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“…These results have been further validated with human placenta-derived MSCs and have been proven to be mediated by inhibition of HDAC2 [130]. Recently, HDAC inhibitors including MS-275, NaBu, TSA, and VPA were reported to promote neuronal differentiation of MSCs through activation of the Wnt signaling pathway [131].…”

Section: Neuronal Differentiationmentioning

confidence: 62%

Control of mesenchymal stem cell biology by histone modifications

Ren

Huang

et al. 2020

Cell Biosci

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Mesenchymal stem cells (MSCs) are considered the most promising seed cells for regenerative medicine because of their considerable therapeutic properties and accessibility. Fine-tuning of cell biological processes, including differentiation and senescence, is essential for achievement of the expected regenerative efficacy. Researchers have recently made great advances in understanding the spatiotemporal gene expression dynamics that occur during osteogenic, adipogenic and chondrogenic differentiation of MSCs and the intrinsic and environmental factors that affect these processes. In this context, histone modifications have been intensively studied in recent years and have already been indicated to play significant and universal roles in MSC fate determination and differentiation. In this review, we summarize recent discoveries regarding the effects of histone modifications on MSC biology. Moreover, we also provide our insights and perspectives for future applications.

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Section: Neuronal Differentiationmentioning

confidence: 62%

Control of mesenchymal stem cell biology by histone modifications

Ren

Huang

et al. 2020

Cell Biosci

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“…The forward and reverse PCR oligonucleotide primers that were selected to amplify the cDNA are listed in Table 1 (Bioneer Co., Daejeon, Korea). RT-PCR products were verified by electrophoresis on 2% agarose gels (Sigma Chemical Co.) The amount of cDNA was normalized based on the level of the ubiquitously expressed glyceraldehyde 3-phosphate ( GAPDH ) to analyze the relative expression of mRNAs [1] , [2] , [3] , [4] , [5] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Data for the effect of histone deacetylase inhibitors on voltage- and ligand-gated ion channel gene expression in neurogenic induced-human adipose tissue-derived mesenchymal stem cells

Jang

Jeong

2018

Data in Brief

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This data article contains descriptive and experimental data on ion channel gene expressions following the histone deacetylase (HDAC) inhibitor treatment of neural induced human adipose tissue-derived mesenchymal stem cells (NI-hADSCs). Following treatment of the HDAC inhibitors, such as MS-275, NaB, TSA, or VPA, the phenotypes of NI-hADSCs exhibit neuron-like features and the neurofilament-L (NFL)-positive cells were increased. The expression of the ion channel marker genes, such as SCN5A, KCNA4, and CACNA1G, was highly increased following treatment with the HDAC inhibitors; however, the expression of others was either decreased or unchanged. For further details and experimental findings please refer to the research article by Jang and Jeong. Histone deacetylase inhibition-mediated neuronal differentiation via the Wnt signaling pathway in human adipose tissue-derived mesenchymal stem cells (Jang and Jeong, 2018) [1].

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“…The mechanism of action of VPA is a rather complex one, but it is likely that it mainly elicits developmental and behavioral alterations by inhibition of histone deacetylase (Moldrich et al, 2013), potentially affecting gene expression and transcription. It engages, among others, the Wnt1 signaling pathway, a robust ''caudalizing'' factor in the rostrocaudal patterning of CNS (Wiltse, 2005;Jang and Jeong, 2018). Prenatal VPA treatment altered the expression in neural stem/progenitor cells of genes associated with cell migration, including CXC motif chemokine receptor 4 (Cxcr4), leading to the ectopic localization of newborn neurons in the hilus of the hippocampus (Sakai et al, 2018).…”

Section: Transcriptional Effectsmentioning

confidence: 99%

Gestational Exposure to Sodium Valproate Disrupts Fasciculation of the Mesotelencephalic Dopaminergic Tract, With a Selective Reduction of Dopaminergic Output From the Ventral Tegmental Area

et al. 2020

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Gestational exposure to valproic acid (VPA) is known to cause behavioral deficits of sociability, matching similar alterations in human autism spectrum disorder (ASD). Available data are scarce on the neuromorphological changes in VPA-exposed animals. Here, we focused on alterations of the dopaminergic system, which is implicated in motivation and reward, with relevance to social cohesion. Whole brains from 7-day-old mice born to mothers given a single injection of VPA (400 mg/kg b.wt.) on E13.5 were immunostained against tyrosine hydroxylase (TH). They were scanned using the iDISCO method with a laser light-sheet microscope, and the reconstructed images were analyzed in 3D for quantitative morphometry. A marked reduction of mesotelencephalic (MT) axonal fascicles together with a widening of the MT tract were observed in VPA treated mice, while other major brain tracts appeared anatomically intact. We also found a reduction in the abundance of dopaminergic ventral tegmental (VTA) neurons, accompanied by diminished tissue level of DA in ventrobasal telencephalic regions (including the nucleus accumbens (NAc), olfactory tubercle, BST, substantia innominata). Such a reduction of DA was not observed in the non-limbic caudateputamen. Conversely, the abundance of TH+ cells in the substantia nigra (SN) was increased, presumably due to a compensatory mechanism or to an altered distribution of TH+ neurons occupying the SN and the VTA. The findings suggest that defasciculation of the MT tract and neuronal loss in VTA, followed by diminished dopaminergic input to the ventrobasal telencephalon at a critical time point of embryonic development (E13-E14) may hinder the patterning of certain brain centers underlying decision making and sociability.

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Histone deacetylase inhibition-mediated neuronal differentiation via the Wnt signaling pathway in human adipose tissue-derived mesenchymal stem cells

Cited by 29 publications

References 38 publications

Control of mesenchymal stem cell biology by histone modifications

Control of mesenchymal stem cell biology by histone modifications

Data for the effect of histone deacetylase inhibitors on voltage- and ligand-gated ion channel gene expression in neurogenic induced-human adipose tissue-derived mesenchymal stem cells

Gestational Exposure to Sodium Valproate Disrupts Fasciculation of the Mesotelencephalic Dopaminergic Tract, With a Selective Reduction of Dopaminergic Output From the Ventral Tegmental Area

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