Histone acetyltransferase 1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells

Barman, Hirak Kumar; Yasuda, Takami; Ono, Tatsuya; Nishijima, Hitoshi; Sanematsu, Fumiyuki; Shibahara, Kei-ichi; Nakayama, Tatsuo

doi:10.1016/j.bbrc.2006.05.079

Cited by 69 publications

(68 citation statements)

References 50 publications

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“…In this regard, budding and fission yeast as well as DT40 vertebrate cells deficient in Hat1, the HAT enzyme that acetylates such positions on free histone H4, are sensitive to DBS DNA-damaging agents. 92,93 Consistent with the notion that Hat1 has a role in nucleosome assembly during DSB repair, mutant cells that are defective in both Hat1 and Asf1 have been shown to be epistatic, suggesting that they function together in the same pathway. 92 However, as indicated above, the recruitment of Saccharomyces cerevisiae Hat1 to the DSB repair sites, with kinetics similar to other recombinational repair factors, suggests that Hat1 may act, in addition to chromatin restoration, to facilitate DNA repair.…”

Section: Restoring Chromatin Structuresupporting

confidence: 55%

“…Thus, inactivation of Hat1 generates yeast and vertebrate cells that are sensitive to DNA DSB-induced agents and defective in HR repair. 92,93 The inability of Hat1 to acetylate nucleosomal substrates and its role RPA protein. This protein is replaced by Rad51 to form nucleoprotein filaments, which are involved in searching for and invading DNA strands in the homologous locus (donor locus).…”

Section: Chromatin Remodeling Activities During Dna Repairmentioning

confidence: 99%

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Chromatin dynamics coupled to DNA repair

Huertas

Sendra²,

Muñoz³

2009

Epigenetics

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In order to protect and preserve the integrity of the genome, eukaryotic cells have developed accurate DNA repair pathways involving a coordinated network of DNA repair and epigenetic factors. The DNA damage response has to proceed in the context of chromatin, a packaged and compact structure that is flexible enough to regulate the accession of the DNA repair machinery to DNA-damaged sites. Chromatin modifications and ATP-remodeling activities are both necessary to ensure efficient DNA repair. Here we review the current progress of research into the importance of chromatin modifications and the ATP-remodeling complex to the DNA damage response, with respect to the sensing and signaling of DNA lesions, DNA repair and the processes that restore chromatin structure.

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Section: Restoring Chromatin Structuresupporting

confidence: 55%

Section: Chromatin Remodeling Activities During Dna Repairmentioning

confidence: 99%

Chromatin dynamics coupled to DNA repair

Huertas

Sendra²,

Muñoz³

2009

Epigenetics

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“…Chicken DT40 cells with a homozygous HAT1 deletion showed a moderate decrease in histone H4 lysine 5 and 12 acetylation specifically in the pool of soluble histones (Barman et al, 2006). However, pulse-labeling experiments will need to be done to confirm that the effect of the HAT1 deletion is actually on the newly synthesized molecules rather than on mature histones that have been displaced from chromatin.…”

Section: Are Type B Histone Acetyltransferases Cytoplasmic or Nuclear?mentioning

confidence: 99%

Hat1: the emerging cellular roles of a type B histone acetyltransferase

2007

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Hat1 is the sole known example of a type B histone acetyltransferase. While it has long been presumed that type B histone acetyltransferases participate in the acetylation of newly synthesized histones during the process of chromatin assembly, definitive evidence linking these enzymes to this process has been scarce. This review will discuss recent results that have begun to shed light on the roles of Hat1 and also address several outstanding questions relating to the cellular function of this enzyme.

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“…When synthesized in cytoplasm, H4 is acetylated at K5 and K12 (Sobel et al, 1995;Benson et al, 2006), these acetyl-groups are removed within 20 min after chromatin assembly (Taddei et al, 1999). In DT40 cells, HAT1 mediates H4 acetylation at these two sites (Barman et al, 2006). However, HAT1 is not required for replication coupled chromatin assembly in vivo (Barman et al, 2006).…”

Section: Restoration Of Chromatin Structure Behind Replication Forkmentioning

confidence: 99%

“…In DT40 cells, HAT1 mediates H4 acetylation at these two sites (Barman et al, 2006). However, HAT1 is not required for replication coupled chromatin assembly in vivo (Barman et al, 2006). Thus, the role of K5 and K12 acetylation in nucleosome assembly remains to be uncovered.…”

Section: Restoration Of Chromatin Structure Behind Replication Forkmentioning

confidence: 99%

Nucleosome assembly and epigenetic inheritance

2010

Protein Cell

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In eukaryotic cells, histones are packaged into octameric core particles with DNA wrapping around to form nucleosomes, which are the basic units of chromatin (Kornberg and Thomas, 1974). Multicellular organisms utilise chromatin marks to translate one single genome into hundreds of epigenomes for their corresponding cell types. Inheritance of epigenetic status is critical for the maintenance of gene expression profile during mitotic cell divisions . During S phase, canonical histones are deposited onto DNA in a replication-coupled manner . To understand how dividing cells overcome the dilution of epigenetic marks after chromatin duplication, DNA replication coupled (RC) nucleosome assembly has been of great interest. In this review, we focus on the potential influence of RC nucleosome assembly processes on the maintenance of epigenetic status.

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Histone acetyltransferase 1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells

Cited by 69 publications

References 50 publications

Chromatin dynamics coupled to DNA repair

Chromatin dynamics coupled to DNA repair

Hat1: the emerging cellular roles of a type B histone acetyltransferase

Nucleosome assembly and epigenetic inheritance

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