Histomorphometric Study of Intratrabecular Osteons in the Iliac Bone in Three Metabolic Bone Diseases.

Abstract

Full-thickness biopsy specimens of iliac bones were submitted to morphometric analysis of intratrabecular osteons to study whether and how much the number and density of osteons increase in some metabolic bone diseases such as osteomalacia (OM), primary hyperparathyroidism (PHPT) and renal osteodystrophy (ROD). The biopsies were taken from 16 patients with OM of various causes, 18 with PHPT, 12 with ROD, and from 41 control cases. All the specimens were methacrylate-embedded, sectioned undecalcified and stained with solochrome cyanine or HE. Morphometry included measurements of the density of osteons and the volume of bone and osteoid, partially assisted with a semiautomatic digital image analyzer. Intratrabecular osteons were found to be more numerous in patients with metabolic disorder than in control. It was shown by discriminant analysis that the elevated number of osteons/cm2 tissue area contributes to the differentiation of abnormal from normal bones. The presence of blood vessels in the osteons indicated the biological significance of osteon formation which extends the metabolic surface of trabeculae, providing a basis for trabecular hypertrophy. intratrabecular osteons; metabolic bone disease; tunneling resorption; iliac bone; histomorphometry Intratrabecular resorption cavities and osteons have been the subject of several studies (Jaffe

Sexual dimorphism and age dependence of osteocyte lacunar density for human vertebral cancellous bone

“…It is also possible that tissue microarchitecture can affect the relationship between osteocyte network and age. Similar to cortical bone, cancellous bone from the iliac crest, proximal femur, and calcaneus regions contains some intratrabecular osteons (Lozupone, 1985; Lozupone and Favia, 1990, 1995; Sato and Byers, 1994) and, like cortical bone (Vashishth et al, 2000), may involve different spatial distribution of osteocytes and different magnitude of age‐related changes than vertebral cancellous bones. In fact, Qiu et al (2002b) show that in iliac crest cancellous bone, the osteocyte density and lacunar number are highest at the surface and these do not decrease with age while the osteocyte density in the central regions are lowest and these undergo an exponential age‐related decrease similar to cortical bone.…”