Histomorphometric study of ethanolic extract of Graptophyllum pictum (L.) Griff. leaves on croton oil-induced hemorrhoid mice: A Javanese traditional anti-hemorrhoid herb

Kusumawati, Idha; Rullyansyah, Subhan; Rohmania,; Rizka, Aisyah Farah; Hestianah, Eka Pramyrtha; Matsunami, Katsuyoshi

doi:10.1016/j.jep.2021.114765

Cited by 4 publications

(7 citation statements)

References 29 publications

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“…pictum has been used traditionally to treat haemorrhoids [10][11][12]. The GPLE has been proven for its anti-haemorrhoid activity [11,12], and flavonoid compound was presumed to be the active ingredients due to its anti-inflammatory and anti-oxidant properties [12,13,16]. Flavonoids work as phlebotonic agent [32,33], that improve haemorrhoid symptoms [34][35][36][37].…”

Section: Resultsmentioning

confidence: 99%

“…Graptophyllum pictum (L.) Griff has been used as traditional medicine as contraceptive, delivery aid; to treat wound swelling, ulcers, abscesses, haemorrhoids [5,6], renal impairments [7], rheumatism, urinary infection, scabies, hepatomegaly, ear diseases and as fertility enhancer [8]. In Indonesia, G. pictum has been used to treat tonsilitis, abscess, rheumatism, breast engorgement, breast abscess [9] and haemorrhoid [10][11][12]. The biological properties of this plant such as anti-haemorrhoid [11,12], analgesic anti-inflammation [13], immunomodulator [14], antioxidant [15,16], anti-plaque [17], oxytocic and anti-implantation [5], antidiabetic activities [18] and nephroprotective effects [6,7] have been scientifically proven.…”

Section: Introductionmentioning

confidence: 99%

“…In Indonesia, G. pictum has been used to treat tonsilitis, abscess, rheumatism, breast engorgement, breast abscess [9] and haemorrhoid [10][11][12]. The biological properties of this plant such as anti-haemorrhoid [11,12], analgesic anti-inflammation [13], immunomodulator [14], antioxidant [15,16], anti-plaque [17], oxytocic and anti-implantation [5], antidiabetic activities [18] and nephroprotective effects [6,7] have been scientifically proven. In addition, these plant leaves contain various chemical components such as tannin, alkaloid, steroid and glycoside [8], phenol (including flavonoid, anthraquinone) [8,12,13,16], saponin, coumarin and sugar [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…The biological properties of this plant such as anti-haemorrhoid [11,12], analgesic anti-inflammation [13], immunomodulator [14], antioxidant [15,16], anti-plaque [17], oxytocic and anti-implantation [5], antidiabetic activities [18] and nephroprotective effects [6,7] have been scientifically proven. In addition, these plant leaves contain various chemical components such as tannin, alkaloid, steroid and glycoside [8], phenol (including flavonoid, anthraquinone) [8,12,13,16], saponin, coumarin and sugar [12,13]. G. pictum leaves extract (GPLE) had been reported for its anti-haemorrhoid activity, and it was assumed that the activity is due to the flavonoid compound which has anti-inflammatory and antioxidant properties [12,13,16].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, these plant leaves contain various chemical components such as tannin, alkaloid, steroid and glycoside [8], phenol (including flavonoid, anthraquinone) [8,12,13,16], saponin, coumarin and sugar [12,13]. G. pictum leaves extract (GPLE) had been reported for its anti-haemorrhoid activity, and it was assumed that the activity is due to the flavonoid compound which has anti-inflammatory and antioxidant properties [12,13,16]. In addition, micronized purified flavonoid fraction was also reported to reduce rectal discomfort, pain and secondary haemorrhage following the haemorrhoidectomy [19].…”

Section: Introductionmentioning

confidence: 99%

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Acute Toxicity Assessment of Graptophyllum Pictum (L.) Griff. Leaves Ethanolic Extract and Its Nanoformulations: Comparative Study of Phytosome and Cyclodextrin Inclusion Complex

KUSUMAWATI¹

2023

FARMACIA

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Graptophyllum pictum (L.) Griff has been used in traditional medicine to treat various diseases and shows great potential for clinical use, but is often limited by poor oral bioavailability and lack of information regarding its safety. In this study, the herbal extract was developed into two forms e.g., phytosome and cyclodextrin complex in order to enhance its bioavailability. This study also examines the toxicity of G. pictum leaves ethanolic extract (GPLE) and its nanoformulations. The GPLE phytosome (GPLE-P) was formed using hydration method. The GPLE cyclodextrin inclusion complex (GPLE-CDIC) was produced through co-precipitation methods. Both formulations produced vesicle in nanometer size. The formation of the vesicles was confirmed through FT-IR and differential scanning calorimetry (DSC) analysis. The in vitro toxicity test showed that GPLE and GPLE-P caused erythrocyte morphological changes at 500 μg/mL, while GPLE-CDIC at 250 μg/mL. Oral administration of all formulations did not cause any erythrocyte morphological changes. The complete blood cell analysis showed no significant difference in the number of blood components from the untreated group compared to the treated group. Both formulations can be safely administered orally without any immediate unwanted effect. RezumatGraptophyllum pictum (L.) Griff a fost folosită în medicina tradițională și prezintă un potențial clinic important, dar utilizarea sa este adesea limitată de biodisponibilitatea orală slabă și lipsa de informații privind siguranța ei. În acest studiu, extractul etanolic de frunze a fost folosit pentru obținerea a două forme farmaceutice diferite, fitozom și complex cu ciclodextrina, pentru a crește biodisponibilitatea. Acest studiu prezintă, de asemenea, toxicitatea extractului etanolic de frunze de G. pictum (GPLE) și nanoformulările sale. Fitozomul GPLE (GPLE-P) a fost obținut prin metoda hidratării. Complexul de incluziune al ciclodextrinei cu GPLE (GPLE-CDIC) a fost obținut prin metode de co-precipitare. Ambele formulări au condus la obținerea de vezicule cu dimensiune nanometrică, fiind confirmate prin analiza FT-IR și calorimetria cu scanare diferențială (DSC). Testul de toxicitate in vitro a arătat că GPLE și GPLE-P au cauzat modificări morfologice ale eritrocitelor la 500 μg/mL, în timp ce GPLE-CDIC la 250 μg/mL. Administrarea orală a tuturor formulărilor nu a provocat modificări morfologice ale eritrocitelor. Analiza completă a celulelor sanguine nu a arătat nicio diferență semnificativă în numărul de componente sanguine din grupul netratat comparativ cu grupul tratat. Ambele formulări pot fi administrate în siguranță pe cale orală, fără efecte imediat nedorite.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Acute Toxicity Assessment of Graptophyllum Pictum (L.) Griff. Leaves Ethanolic Extract and Its Nanoformulations: Comparative Study of Phytosome and Cyclodextrin Inclusion Complex

KUSUMAWATI¹

2023

FARMACIA

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Integrated genomic network analysis revealed potential of a druggable target for hemorrhoid treatment

Adikusuma,

Firdayani,

Irham

et al. 2023

Saudi Pharmaceutical Journal

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Phytochemical constituents, antioxidant and antibacterial activities of Plectocephalus varians (A. Rich.) C. Jeffrey ex Cufod root extracts

Gashaye¹,

Birhan²

2023

BMC Complement Med Ther

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Plants have been used to treat diverse types of diseases in different cultural groups around the globe. In this regard, the root of Plectocephalus varians (P. varians) is claimed to have a beneficiary effect in treating cancer and hemorrhoids in Ethiopia. Hence, this study aimed at the phytochemical investigation, antioxidant, and antibacterial activities of n-hexane, acetone, and methanolic extracts of P. varians root. The different crude extracts of P. varians were obtained through maceration technique. The total phenolic content (TPC) and total flavonoid contents (TFC) of the extracts were estimated using Folin-Ciocalteu Reagent (FCR) and aluminum chloride colorimetric assays, respectively. The antioxidant activities of the extracts were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) assays. The antibacterial activities of the extracts were assessed by using disc diffusion method. The results echoed the presence of alkaloids, saponins, flavonoids, steroids, phenols, tannins, anthraquinones, terpenoids, polyphenols, and glycosides in the root of P. varians. The methanolic root extract (MRE) had the highest TPC (107.18 mg GAE/g) and TFC (120.194 mg QE/g) followed by acetone root extract (ARE) (TPC = 98.68 mg GAE/g; TFC = 64.038 mg QE/g) and n-hexane root extract (HRE) (TPC = 12.39 mg GAE/g; TFC = 9.917 mg QE/g). The DPPH radical scavenging and FRAP assays demonstrated the antioxidant effects of HRE (IC50 = 681.75 ppm; EC50 = 60.65 ppm), ARE (IC50 = 165.73 ppm; EC50 = 51.67 ppm) and MRE (IC50 = 132.06 ppm; EC50 = 30.97 ppm) of P. varians. Furthermore, the root fractions elicited pronounced dose-dependent growth inhibition against Staphylococcus aureus, Streptococci pyogenes, Escherichia coli, and Klebsiella pneumoniae with mean zone of inhibition (MZI) ranging from 11 ± 0.38 to 20 ± 0.04 mm at 800 ppm. Overall, the present study provides ethnopharmacological evidence suggesting the medicinal importance of P. varians. The results also call for further bioassay-guided phytochemical screening and in vitro and/or in vivo bioactivity testing.

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Histomorphometric study of ethanolic extract of Graptophyllum pictum (L.) Griff. leaves on croton oil-induced hemorrhoid mice: A Javanese traditional anti-hemorrhoid herb

Cited by 4 publications

References 29 publications

Acute Toxicity Assessment of Graptophyllum Pictum (L.) Griff. Leaves Ethanolic Extract and Its Nanoformulations: Comparative Study of Phytosome and Cyclodextrin Inclusion Complex

Acute Toxicity Assessment of Graptophyllum Pictum (L.) Griff. Leaves Ethanolic Extract and Its Nanoformulations: Comparative Study of Phytosome and Cyclodextrin Inclusion Complex

Integrated genomic network analysis revealed potential of a druggable target for hemorrhoid treatment

Phytochemical constituents, antioxidant and antibacterial activities of Plectocephalus varians (A. Rich.) C. Jeffrey ex Cufod root extracts

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