Histomorphometric evaluation of seminiferous tubules and stereological assessment
            of germ cells in testes following administration of aqueous leaf-extract of Lawsonia
            inermis on aluminium-induced oxidative stress in adult Wistar rats

Ts, Olawuyi; Vo, Ukwenya; Aga, Jimoh; Kb, Akinola

doi:10.5935/1518-0557.20180080

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…Severe hypospermatogenesis or absolute arrest of spermatogenesis as the bundle of spermatozoa seen is distorted compared to those of baseline. These findings were in line with the reports from Olawuyi et al (2019) and Oteiza et al (1999). In this current study, we observed swelling, congestion, and few areas of ischemic necrosis.…”

Section: Discussionsupporting

confidence: 94%

The mitigating effect of Ananas comosus on aluminum-induced oxidative stress on the testes of adult male Wistar rats

Leko

Olawuyi

Okon

2021

JoBAZ

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Background The mitigating effect of Ananas comosus (pineapple juice) extract on an aluminum-induced testicular toxicity in male Wistar rats was examined in this study. Thirty healthy adult male Wistar rats with an average weight of 200 g were grouped into six groups; distilled water and 1 ml of pineapple juice extracts were administered to the control and treated animals respectively for 3 weeks. The control group was given rat pellets and distilled water. Negative control was given 100mg/kg/d Aluminum Chloride and pellets; Group 1 was given 100mg/kg/d and 1ml of pineapple juice in distilled water orally; Group 2 was given 100mg/kg/d Aluminum Chloride and 1.5 ml of in distilled water orally; group 3 was given 100 mg/kg/day aluminum chloride and 2 ml of pineapple juice in distilled water orally; group 4 was given 100 mg/kg/day aluminum chloride and 2.5 ml of pineapple juice in distilled water orally. Testicular histology, semen parameters, and testosterone were assessed. Results This study showed a significant (P < 0.05) decrease in testicular volume, motile sperm count, concentration, total count, progressive evaluation, and morphology in the negative control group relative to the normal control and extract control groups. In the groups co-treated with aluminum chloride and Ananas comosus extract, there was improvement in sperm volume, motility, total count, progressive assessment, and morphology. There was also a statistical decrease (P < 0.05) in testosterone hormone in the negative control group, but there was an increase in the aluminum chloride and Ananas comosus extract co-treated groups. Similarly, in co-treated aluminum chloride and Ananas comosus extract, the degenerative seminiferous tubule histoarchitecture due to aluminum chloride in the negative control group was enhanced. Conclusion Based on this current study, it was evident that aluminum chloride induced oxidative stress and retarded reproduction in males whereas Ananas comosus mitigated reproduction in males by improving sperm parameters and microarchitecture of the testes.

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Section: Discussionsupporting

confidence: 94%

The mitigating effect of Ananas comosus on aluminum-induced oxidative stress on the testes of adult male Wistar rats

Leko

Olawuyi

Okon

2021

JoBAZ

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“…Aluminum can produce reproductive toxicity in female and male rodents. Studies that were GLP compliant and followed Sakr et al (2017) 8 339 Adedosu et al (2018) 16 339 Mouro et al (2018) 0.000134, 0.00067, 20, or 80 0.015, 0.075, 2240, or 8960 Sun et al (2018) 25, 52, or 103 3103, 6205, or 12,411 Gomes et al (2019 40 323 Olawuyi et al (2019) 0.2 4.2 Yuan et al (2019) 15, 30 or 59 1653, 3307, or 6613 Cao et al (2020) 20 560 G€ uvenç et al 202014 962 Sajjad et al (2020) 20.2 909 E. Study found some statistically significant results from higher exposure Melograna and Yokel (1984) 3400 68,000 Oneda et al (1994) 114, 285, 570, or 114069,312, 173,280, 346,560, or 693,120 Llobet et al (1995 3600, 7200, or 14,400 72,000, 144,000, or 288,000 Guo et al (2001) 13,000 or 35,000 182,000 or 490,000 Guo et al (2002) 13,000 or 35,000 182,000 or 490,000 Guo et al (2005a) 35,000 420,000 Guo et al (2005b) 1470 or 2730 20,580 or 38,220 Guo et al (2006) 1470 or 7350 20,580 or 102,900 Reza and Palan (2006) 10,100 202,000 Khattab (2007) 3030 or 6060 53,025 or 105,050 Kutlubay et al (2007) 339 4790 Guo et al (2009) 1414 or 7070 19,796 or 98,980 Cui et al (2009) 7214, 10,821, or 14,428 72,140, 108,210, or 144,280 Buraimoh et al (2012b 768 42,986 Abdel-Moneim (2013) 5050 5050 Chen et al (2014) 577, 1154, or 17311731, 3464, or 5194 D'Souza et al (2014) 6600, 13,200, or 19,800 46,200, 92,400, or 138,600 Maghraoui et al (2014 1778 24,892 Al…”

Section: Discussionmentioning

confidence: 99%

Aluminum reproductive toxicity: a summary and interpretation of scientific reports

Yokel

2020

Critical Reviews in Toxicology

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Publications addressing aluminum (Al)-induced reproductive toxicity were reviewed. Key details were compiled in summary tables. Approximate systemic Al exposure, a measure of bioavailability, was calculated for each exposure, based on the Al percentage in the dosed Al species, Al bioavailability, and absorption time course reports for the exposure route. This was limited to laboratory animal studies because no controlled-exposure human studies were found. Intended Al exposure was compared to unintended dietary Al exposure. The considerable and variable Al content of laboratory animal diets creates uncertainty about reproductive function in the absence of Al. Aluminum-induced reproductive toxicity in female mice and rats was evident after exposure to !25-fold the amount of Al consumed in the diet. Generally, the additional daily Al systemic exposure of studies that reported statistically significant results was greater than 100-fold above the typical human daily Al dietary consumption equivalent. Male reproductive endpoints were significantly affected after exposure to lower levels of Al than females. Increased Al intake increased fetus, placenta, and testes Al concentrations, to a greater extent in the placenta than fetus, and, in some cases, more in the testes than placenta. An adverse outcome pathway (AOP) was constructed for males based on the results of the reviewed studies. The proposed AOP includes oxidative stress as the molecular initiating event and increased malondialdehyde, DNA and spermatozoal damage, and decreased blood testosterone and sperm count as subsequent key events. Recommendations for the design of future studies of reproductive outcomes following exposure to Al are provided.

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“…During the developmental process of spermatogonia, their morphology, function, and quantity are controlled by various biological and metabolic factors, including testicular hormones, DNA damage repair, mitochondrial function, and autophagy [32,33]. The proliferation, apoptosis, and differentiation of spermatogonia can impact sperm vitality and ultimately contribute to the development of asthenozoospermia [34]. Studies have shown that Cuscuta chinensis Lam.…”

Section: Discussionmentioning

confidence: 99%

C9orf72 Overexpression in Asthenozoospermia: Implications for Spermatogonia Proliferation through mitophagy and glucose metabolism

Lu,

Xu,

Zhao

et al. 2023

Preprint

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Background The role of the C9orf72 gene in sperm function and asthenozoospermia remains unclear. This study aimed to investigate whether the expression of C9orf72 was abnormal in patients with asthenozoospermia and its effects on spermatogonia proliferation. Methods Semen samples were collected from 27 men with asthenozoospermia and 31 normal men. Immunofluorescence and Western blotting were employed to detect the protein expression of C9orf72 in semen samples. Mouse spermatogonia (GC-1 spg) transfected with C9orf72-overexpressing lentivirus were assessed for cell viability, apoptosis, ROS levels, mitochondrial membrane potential changes, colocalization between autophagosomes and mitochondria, glucose content and glucose energy metabolism-related protein expression. The effect of the mitophagy inhibitor Mdivi-1 on C9orf72-induced apoptosis in GC-1 spg cells was investigated. Results The results showed that the C9orf72 protein was significantly upregulated in asthenozoospermic semen samples and negatively correlated with sperm progressive motility. Overexpression of C9orf72 inhibited proliferation, increased apoptosis, and elevated ROS levels in GC-1 spg cells. Enhanced mitophagy was observed in C9orf72-overexpressing cells, as indicated by mitochondrial membrane potential reduction, decreased MitoTracker Red CMXRos-labeled mitochondria, and increased LC-3-labeled autophagy. Moreover, glucose energy metabolism was impaired in C9orf72-overexpressing cells, with decreased glucose uptake and reduced protein expression of GLUT1, GLUT3, HK2 and LDHA. The mitophagy inhibitor Mdivi-1 partially reversed C9orf72-induced apoptosis in GC-1 spg cells. Conclusions This study demonstrates that C9orf72 is significantly overexpressed in asthenozoospermic semen samples and negatively correlated with sperm progressive motility. C9orf72 overexpression inhibits spermatogonia proliferation, and the mechanism by which it inhibits the proliferation of spermatogonia may be through promoting mitophagy and inhibiting glucose metabolism, representing a potential regulatory mechanism underlying asthenozoospermia.

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Histomorphometric evaluation of seminiferous tubules and stereological assessment of germ cells in testes following administration of aqueous leaf-extract of Lawsonia inermis on aluminium-induced oxidative stress in adult Wistar rats

Cited by 5 publications

References 23 publications

The mitigating effect of Ananas comosus on aluminum-induced oxidative stress on the testes of adult male Wistar rats

The mitigating effect of Ananas comosus on aluminum-induced oxidative stress on the testes of adult male Wistar rats

Aluminum reproductive toxicity: a summary and interpretation of scientific reports

C9orf72 Overexpression in Asthenozoospermia: Implications for Spermatogonia Proliferation through mitophagy and glucose metabolism

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