Histomorphometric characterization of the intertubular compartment in the testes of the bat Sturnira lilium

Morais, Danielle Barbosa; Barros, Mirlaine S.; Freitas, Mariella Bontempo; Paula, Tarcízio Antônio Rêgo de; Matta, S. L. P.

doi:10.1016/j.anireprosci.2014.03.008

Cited by 12 publications

(26 citation statements)

References 31 publications

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“…molossus [27] and in S . lilium [28], however higher than in Myotis levis [35] and within the recorded for other mammals [22, 29–33]. The Leydigosomatic index was similar to that observed in other bats and higher than the observed in larger animals [21, 27, 28, 30, 32, 33].…”

Section: Discussionsupporting

confidence: 72%

“…In vampire bat, the percentage of Leydig cells was lower compared to frugivorous and insectivorous bats and consequently the percentage of lymphatic space was larger [21, 27, 28], yet within the numbers reported for other mammals [22, 29–33]. The larger investment in Leydig cells found in bats might also be associated to their reproductive behavior, as high levels of testosterone would be required for the maintenance of spermatogenesis and male dominance in harem colonies [23, 27, 28]. This steroidogenic capacity is reinforced by the large number of mitochondria in their cytoplasm, being that the shape of this organelle frequently varies from spherical to elongated or tubular in steroidogenic cells [34].…”

Section: Discussionmentioning

confidence: 99%

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The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view

et al. 2017

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Among all bat species, Desmodus rotundus stands out as one of the most intriguing due to its exclusively haematophagous feeding habits. However, little is known about their spermatogenic cycle. This study aimed at describing the spermatogenic process of common vampire bats through testicular histomorphometric characterization of adult specimens, spermatogenic production indexes, description of stages of the seminiferous epithelium cycle and estimative of the spermatogenic process duration. Morphometrical and immunohistochemical analyzes for bromodeoxiuridine were conducted under light microscopy and ultrastructural analyzes were performed under transmission electron microscopy. Vampire bats showed higher investment in gonadal tissue (gonadosomatic index of 0.54%) and in seminiferous tubules (tubulesomatic index of 0.49%) when compared to larger mammals. They also showed a high tubular length per gram of testis (34.70 m). Approximately half of the intertubular compartment was found to be comprised by Leydig cells (51.20%), and an average of 23.77x106 of these cells was found per gram of testis. The germline cells showed 16.93% of mitotic index and 2.51% of meiotic index. The overall yield of spermatogenesis was 60% and the testicular spermatic reserve was 71.44x107 spermatozoa per gram of testis. With a total spermatogenesis duration estimated at 37.02 days, vampire bats showed a daily sperm production of 86.80x106 gametes per gram of testis. These findings demonstrate a high sperm production, which is commonly observed in species with promiscuous mating system.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view

et al. 2017

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“…In other studies, similar weights were found for the three species (A. lituratus ~65 g- Duarte & Talamoni, 2010;P. lineatus ~ 25 g-Beguelini, Puga, Taboga, et al, 2013;and S. lilium 20-22.6 g-Álvarez-Guerrero, Medrano, & Moreno-Mendoza, 2014;Morais, Barros, Freitas, Paula, & Matta, 2014) in other biomes (especially Atlantic Forest). The only difference between dry and rainy periods was in the body weight of S. lilium animals, which was higher in the dry period.…”

Section: Discussionsupporting

confidence: 74%

“…In our study, A. lituratus is the largest species (65.1–67.7 g), followed by P. lineatus (20.5–25.7 g) and S. lilium (21.3–23.0 g), which have similar body weights. In other studies, similar weights were found for the three species ( A. lituratus ~65 g—Beguelini, Puga, Taboga, & Morielle‐Versute., ; Duarte & Talamoni, ; P. lineatus ~ 25 g—Beguelini, Puga, Taboga, et al, ; and S. lilium 20–22.6 g—Álvarez‐Guerrero, Medrano, & Moreno‐Mendoza, ; Morais, Barros, Freitas, Paula, & Matta, ) in other biomes (especially Atlantic Forest). The only difference between dry and rainy periods was in the body weight of S. lilium animals, which was higher in the dry period.…”

Section: Discussionmentioning

confidence: 97%

Evaluation of reproductive parameters in male Neotropical bats during dry and rainy months in a specific area of the Cerrado biome

Brito

Amaral

Aguiar

et al. 2020

Anat Histol Embryol

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The aim of this study was to analyse the reproductive aspects of male bats of three common species of the Phyllostomidae family: Artibeus lituratus, Platyrrhinus lineatus and Sturnira lilium, during dry and rainy months in a specific area of the Cerrado biome. Body weight was significantly higher during the dry months for S. lilium. The gonadosomatic index (GSI) and testicular weight were not significantly different between dry and rainy periods. The tubular parameters were significantly bigger in A. lituratus than in the other two species during both periods. No difference in the tubular/interstitial ratio was observed in any of the species during both periods. In both periods, all sperm cells and germ cell developmental stages were visible on seminiferous tubules whereas sperm cells were observed in epididymides of all sampled animals. The percentage of morphologically normal sperm was low (35%–60%), with no difference between periods. Spermatozoa from A. lituratus presented a leaf‐shaped head, while the head was round‐shaped in the other two species. In conclusion, our data suggest that males from the three studied species did not present reproductive latency during the most critical weather periods (dry and rainy months) in the metropolitan region of Brasilia, Brazil.

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“…Despite bat diversity, there is limited information on function, physiological control, and cyclicality of spermatogenesis (Krutzsch 2000). Testicular positioning is an aspect commonly reported in studies addressing male bat reproduction, as these organs present seasonal variation between the inguinal and abdominal region (Lima Junior et al 2014); however, sexual activity cannot be established only with the external morphological features of the gonads, reason why evaluation of the testis morphological parameters can present inferences about the dynamics of gonadal development and species reproductive capacity (Beguelini et al 2009, 2011, 2013d, 2016, Morais et al 2013a, 2014a, 2014b, Lima Junior et al 2014.…”

Section: Introductionmentioning

confidence: 99%

Seasonal influence on testicular morphophysiological parameters of bat Carollia perspicillata in fragments of the Atlantic Forest, northeastern Brazil

Arandas

Teixeira

et al. 2019

Pesq. Vet. Bras.

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ISSN 0100-736X (Print) ISSN 1678-5150 (Online) RESUMO.-[Influência sazonal sobre os parâmetros morfofisiológicos testiculares de Carollia perspicillata (Chiroptera: Phyllostomidae) em fragmentos florestais de Mata Atlântica, Nordeste do Brasil.] Os morcegos pertencem a ordem Chiroptera, família Phyllostomidae, e apresentam ampla diversidade de estratégias reprodutivas.Entretanto, as informações relacionadas à biologia reprodutiva dos machos são escassas, principalmente no Nordeste do Brasil. Dessa forma, o trabalho avaliou a histomorfometria sazonal testicular de Carollia perspicillata em fragmentos de Mata Atlântica de Pernambuco. Para tanto, os machos adultos foram coletados, eutanasiados para a remoção dos testículos, e posteriormente submetidos à técnica histológica de rotina. As análises histomorfométricas avaliaram as áreas de ocupação do compartimento tubular e intertubular, assim como a quantificação dos espermatócitos, espermátides arredondadas, espermátides alongadas, células de Sertoli e de Leydig. Os resultados indicaram que a espécie apresenta sazonalidade reprodutiva, visto que maiores médias significativas dos parâmetros testiculares foram encontradas na estação chuvosa, que é um período de maior disponibilidade de recursos alimentares. Tais ABSTRACT.-Arandas M.Bats belong to the order Chiroptera, family Phyllostomidae, and present a wide diversity of reproductive strategies. However, information on the reproductive biology of male bats is scarce, mainly in the Northeast Region of Brazil. Thus, this study evaluated the seasonal testicular histomorphometry of the bat Carollia perspicillata in fragments of the Atlantic Forest in Pernambuco state. To this end, adult males were collected, euthanized for removal of the testicles, and later submitted to a routine histological technique. Histomorphometric analysis included assessment of the areas of tubular and intertubular compartment occupation, as well as quantification of spermatocytes, rounded spermatids, elongated spermatids, and Sertoli and Leydig cells. Results indicated that this bat species presents reproductive seasonality, because significantly higher averages of the testicular parameters were observed in the rainy season, which is a period of greater availability of food resources. Such inferences indicate that there is a synchrony between peak spermatogenesis and hormonal inversion in the months of high precipitation; furthermore, a higher carrying capacity of the Sertoli cells is noted. C. perspicillata males possibly present greater sperm and androgenic activity in the rainy season, associated with increased tubular area and number of spermatogenic cells, as well as with the intertubular area and number of Leydig cells, respectively.

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Histomorphometric characterization of the intertubular compartment in the testes of the bat Sturnira lilium

Cited by 12 publications

References 31 publications

The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view

The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view

Evaluation of reproductive parameters in male Neotropical bats during dry and rainy months in a specific area of the Cerrado biome

Seasonal influence on testicular morphophysiological parameters of bat Carollia perspicillata in fragments of the Atlantic Forest, northeastern Brazil

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