Histomorphometric and Microtomographic Evaluation of the Effects of Hyperbaric Oxygen and Systemic Ozone, Used Alone and in Combination, on Calvarial Defect Healing in Rats

Kan, Bahadır; Şençimen, Metin; Bayar, Gürkan Raşit; Korkusuz, Petek; Coşkun, Abdullah Tuğrul; Korkmaz, Ahmet; Bahador, Elham; Zerener, Tamer

doi:10.1016/j.joms.2015.02.018

Cited by 13 publications

(11 citation statements)

References 28 publications

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“…Similarly, microtomographicanalysis showed larger volumes of new bone in all experimental groups compared with the control group. The use of hyperbaric oxygen and ozone, were shown to be effective in increasing bone healing 23) . Buyuk et al investigated the of effects of different concentrations of ozone therapy on bone regeneration in response to an expansion of the inter-premaxillary suture in rats.…”

Section: Discussionmentioning

confidence: 99%

Effects of Gaseous Ozone Treatment on Bone Regeneration in Femoral Defect Model in Rats

Duman

Davul

Gökçe

et al. 2017

J. Hard Tissue Biology.

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The purpose of this study was to investigate the effect of ozone therapy in rat femoral fractures.Following the right open femoral fractures and intramedullary Kirschner wire fixation of the fracture in 60 male Wistar albino rats, the animals were divided into two groups of 30 animals each: ozone therapy group (n=30); non-treatment (control) group (n=30). Ozone was administered by rectal insufflation. After the rats were sacrified fracture healing was assessed by biomechanical and histopathologic evaluation. Histological and mechanical findings indicated that the ozone therapy had a significantly positive effect on bone healing.

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Section: Discussionmentioning

confidence: 99%

Effects of Gaseous Ozone Treatment on Bone Regeneration in Femoral Defect Model in Rats

Duman

Davul

Gökçe

et al. 2017

J. Hard Tissue Biology.

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“…Insufficient work has been carried out to show a relationship between exposure to ozone application and calvarial defect in rats (11,14,15,64). Kan et al aimed to compare the efficacy of hyperbaric oxygen and systemic ozone, used separately and in combination, on the healing of bone defects.…”

Section: Discussionmentioning

confidence: 99%

“…However, they also indicated that partial intramembranous and endochondral ossification were observed during the calvarial bone defect healing process. Their histomorphometric analysis showed more new bone formation in all experimental groups compared with the control group on days 5, 15, and 30 (11). Similarly, the effects of systemic and topical ozone applications on alveolar bone healing after tooth extraction were investigated.…”

Section: Discussionmentioning

confidence: 99%

“…In an experimental study, they asserted that both hyperbaric oxygen and ozone depicted the same efficacy to augment bone healing and that the concomitant use of them would be more effective (11). Laçin et al (39) confirmed that ozone has healing effects on bone defects at 4and 8-week intervals, indicating that graft and ozone application is the best practice for new bone formation.…”

Section: Discussionmentioning

confidence: 99%

“…In an experimental study, it has been reported that hyperbaric oxygen and ozone are equally effective on bone development in rats induced with calvarial defects (11). In a clinical study with topical ozone application, alveolar bone healing is accelerated using postoperative long-term systemic ozone application (12).…”

Section: Introductionmentioning

confidence: 99%

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Effects of alloplastic graft material combined with a topical ozone application on calvarial bone defects in rats

et al. 2020

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Background:The present study presents the evaluation of the damage in the bone tissue resulting from a calvarial defect in rats and the efficiency of exposure to an ozone application with an alloplastic bone graft on the calvarial bone damage. Materials and methods:Wistar male rats (n = 56) were divided into four groups: a control group (n = 14), defect and ozone group (n = 14), defect and graft group (n = 14), and defect, graft, and ozone group (n = 14). Under anaesthesia, a circular full-thickness bone defect was created in all groups, and the experimental groups were further divided into two sub-groups, with seven rats in each group sacrificed at the end of the 4 th and 8 th weeks. Bone samples were dissected, fixed in 10% formalin solution, and decalcified with 5% ethylene-diaminetetraacetic acid (EDTA). After the routine follow-up on tissues, immunostaining of osteopontin and osteonectin antibodies was applied to sections and observed under a light microscope. Results:The control group exhibited osteopontin and osteonectin expression in fibroblasts and inflammatory cells at the end of the 4 th week with an acceleration at the 8 th week. Ozone administration elucidated new trabecular bone formation by increasing osteoblastic activity.Lastly, our observations underscore that a combination of allograft and ozone application increased the osteoblast, osteocyte, and bone matrix development at the 4 th and 8 th weeks. Conclusions:Exposure to an ozone application with an alloplastic bone graft on calvarial bone damage may induce osteoblastic activity, matrix development, mature bone cell formation, and new bone formation in rats.

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Topical Wound Treatments and Wound‐Care Products

Jacobsen¹

2016

Equine Wound Management

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Histomorphometric and Microtomographic Evaluation of the Effects of Hyperbaric Oxygen and Systemic Ozone, Used Alone and in Combination, on Calvarial Defect Healing in Rats

Cited by 13 publications

References 28 publications

Effects of Gaseous Ozone Treatment on Bone Regeneration in Femoral Defect Model in Rats

Effects of Gaseous Ozone Treatment on Bone Regeneration in Femoral Defect Model in Rats

Effects of alloplastic graft material combined with a topical ozone application on calvarial bone defects in rats

Topical Wound Treatments and Wound‐Care Products

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