Histomorphometric and Biochemical Correlates of Arterial Procollagen Gene Expression During Vascular Repair After Experimental Angioplasty

Karim, M.Asad; Miller, D. Douglas; Farrar, M. A.; Eleftheriades, Elias G.; Reddy, B.H.; Breland, Clyniece M.; Samarel, Allen M.

doi:10.1161/01.cir.91.7.2049

Cited by 58 publications

(27 citation statements)

References 25 publications

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“…[29,30,31] In fact, the fibrosis of the aortic wall would be due to the presence of collagen, [32] which is dominant in the proliferative intima. [33] Our qualitative and quantitative study on the phenotypic expression of collagen has shown that cultured SMCs of the normal and diabetic Psammomys obesus synthesise more type I than type III collagen, in accord with the results of Layman et al [34] [36] have shown that SMC produced essentially collagen I and III. Ang et al [37] have observed an activation of mRNA coding 1 (I) and czl (III) chains of procollagen in rabbit synthetic SMCs. Compared with control, the diabetic state induced a large increase in type I and type III collagen biosynthesis and secretion.…”

Section: Discussionsupporting

confidence: 86%

“…Karim et al. [33] have noted equally an increase of mRNA coding for 01 (I) zl (III) chains after 30 days of experimental angioplasty. Katsuda et al [32] have shown the coexistence of the two phenotypes in the intimal lesions, but type I collagen would stimulate the SMC migration, t38] During the development of the atherosclerotic process, many authors showed that collagen molecules are also subject to qualitative alterations.…”

Section: Discussionmentioning

confidence: 94%

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Non Insulin Dependent Diabetes in Sand Rat (Psammomys obesus) and Production of Collagen in Cultured Aortic Smooth Muscle Cells. Influence of Insulin

Aouichat-Bouguerra

Bourdillon²,

Dahmani

et al. 2000

Journal of Diabetes Research

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 94%

Non Insulin Dependent Diabetes in Sand Rat (Psammomys obesus) and Production of Collagen in Cultured Aortic Smooth Muscle Cells. Influence of Insulin

Aouichat-Bouguerra

Bourdillon²,

Dahmani

et al. 2000

Journal of Diabetes Research

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“…Restenosis emerges from the migration of smooth muscle cells from the arterial media to the intima, where they change to a synthetic phenotype, produce extracellular matrix, and proliferate, thereby resulting in a stenosis within the vessel lumen (21,22). There has been a consensus that inhibition of smooth muscle cell proliferation can reduce intimal hyperplasia after angioplasty (23,24).…”

Section: Introductionmentioning

confidence: 99%

Elucidating the Inhibitory Mechanisms of Magnolol on Rat Smooth Muscle Cell Proliferation

Chen

et al. 2005

J Pharmacol Sci

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Abstract. The pathological mechanism of percutaneous transluminal coronary angioplastyinduced restenosis has been attributed to outgrowth of vascular smooth muscle cells. Pretreatment with antioxidants has been shown to reduce restenosis. Magnolol, an active compound of Magnolia officinalis, has exhibited approximately 1,000 times more potent antioxidant effects than alpha-tocopherol. In this study, we demonstrate, using cytometric analysis, an approximate 61% reduction of smooth muscle cells progressing to the S-phase by 0.05 mg / ml of magnolol. A BrdU incorporation assay also showed a significant reduction (73%) of DNA synthesis using 0.05 mg / ml of magnolol. The protein level of the proliferating cell nuclear antigen was suppressed by approximately 48% using 0.05 mg / ml of magnolol. This was in agreement with the promoter activity of nuclear factor-kappa B, which was also attenuated by 0.05 mg / ml of magnolol. Since receptor interacting protein and caspase-3 protein expression levels were both increased by magnolol in a dose-dependent manner, the apoptotic pathway may mediate the inhibition of cell growth. Our finding that malondialdehyde formation was significantly inhibited by 0.05 mg / ml of magnolol further supported the antioxidant effect of magnolol. These studies suggest that magnolol might be a potential pharmacological reagent in preventing balloon injuryinduced restenosis.

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“…The lesions contain a fibromuscular cap covering a core composed of extracellular lipid and cell debris and show marked depositions of collagenous matrix. [37][38][39][40][41] In the present study we focused on the expression of network-forming collagen type VIII mRNA in comparison with fibrillar collagens (types I and III collagen), and in relation to TGF-␤1 and MMP-I in rabbit iliac arteries after a 1% cholesterol diet with and without previous balloon injury. 37,38 The mRNA expression was followed by in situ hybridization, for type VIII collagen also by northern blot analysis, and with immunohistochemistry to examine the corresponding protein.…”

mentioning

confidence: 99%

Expression of Type VIII Collagen After Cholesterol Diet and Injury in the Rabbit Model of Atherosclerosis

Plenz

Dorszewski

Breithardt

et al. 1999

ATVB

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Abstract-This study presents an analysis of the expression of type VIII collagen mRNA in response to cholesterol diet and balloon injury in the rabbit iliac artery. The design of the animal experiments was as follows: 28 male New Zealand White rabbits were divided into the 3 different treatment groups. Group 1 received regular chow; group 2 was fed with a 1% cholesterol diet for 6 weeks and normal chow for 5 weeks; and group 3 underwent balloon injury, then 6 weeks of a 1% cholesterol diet, which was followed by 5 weeks of normal chow. The expression pattern of type VIII collagen mRNA was compared with that of the fibrillar collagen types I and III, transforming growth factor-␤1, a factor known to exert the most potent stimulatory effect on collagen synthesis in vitro, and matrix metalloproteinase 1, a collagen-degrading enzyme. The cholesterol diet resulted in an upregulation of type VIII collagen, fibrillar collagens, transforming growth factor-␤1, and matrix metalloproteinase I in the adventitia. Although the number of type VIII collagen mRNA-expressing cells in the media increased, no significant difference in overall expression levels was detectable by northern blot analysis. The ratio of medial smooth muscle cells expressing type VIII collagen mRNA to those expressing type I and type III collagen mRNA (CVIII:CI:CIII) changed from 1:1.88:0.03 in the normal media to 1:0.78:0.29. When cholesterol feeding was preceded by balloon injury, type VIII collagen mRNA expression concomitant with the fibrillar collagens was further upregulated over and above that level reported after cholesterol diet alone. In general, low levels of transforming growth factor-␤1 mRNA correlated with high expression of matrix metalloproteinase I. Our study indicates that a cholesterol diet resulted in a balanced reorganization of the collagen composition but did not result in marked collagen accumulation. This may provide an extracellular environment that favors migration and proliferation processes during early atherogenesis. It also demonstrates that type VIII collagen is highly expressed and deposited at later stages, and this may be linked to processes such as tissue reorganization during vascular repair and plaque stabilization. Key Words: rabbit Ⅲ balloon injury Ⅲ extracellular matrix remodeling Ⅲ collagen Ⅲ collagenase Ⅲ transforming growth factor-␤ Ⅲ stenosis A therosclerosis involves pathological changes in the normal structure and function of the arterial wall that depend critically on interactions between vessel wall cells and their extracellular environment. The principal vascular cell type responsible for extracellular matrix (ECM) metabolism is the smooth muscle cell (SMC). 1,2 Medial SMCs, expressing the contractile phenotype, are embedded in an ECM composed of basement membrane molecules, elastin, and collagens. 3 These cells show low proliferative activity and do not migrate. During early atherogenesis, SMCs differentiate from the contractile to the synthetic phenotype. This transition is accompanied by the onset of SMC...

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Histomorphometric and Biochemical Correlates of Arterial Procollagen Gene Expression During Vascular Repair After Experimental Angioplasty

Cited by 58 publications

References 25 publications

Non Insulin Dependent Diabetes in Sand Rat (Psammomys obesus) and Production of Collagen in Cultured Aortic Smooth Muscle Cells. Influence of Insulin

Non Insulin Dependent Diabetes in Sand Rat (Psammomys obesus) and Production of Collagen in Cultured Aortic Smooth Muscle Cells. Influence of Insulin

Elucidating the Inhibitory Mechanisms of Magnolol on Rat Smooth Muscle Cell Proliferation

Expression of Type VIII Collagen After Cholesterol Diet and Injury in the Rabbit Model of Atherosclerosis

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