Histomorphometric alteration of knee articular cartilage and serum alkaline phosphatase in young female mice by chronic supplementation with soybean

Fazelipour, Simin; Tootian, Zahra; Matini, Esfandiar; Hadipour-Jahromy, Mahsa

doi:10.1002/ptr.3327

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…The relative area of the cartilage and the chondrocyte counts were determined using a photomicroscope at a magnification of ×400 from each scanned section (28). The relative area of the cartilage was calculated using AxioVision software, by drawing a circle with a diameter extending from zone 1 to zone 3.…”

Section: Methodsmentioning

confidence: 99%

A promising novel formulation for articular cartilage regeneration: Preclinical evaluation of a treatment that produces SOX9 overexpression in human synovial fluid cells

Delgado‐Enciso

García²,

Hernández

et al. 2017

Mol Med Report

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Osteoarthritis (OA) is a chronic disorder of synovial joints, in which there is progressive softening and disintegration of the articular cartilage. OA is the most common form of arthritis, and is the primary cause of disability and impaired quality of life in the elderly. Despite considerable medical necessity, no treatment has yet been proven to act as a disease-modifying agent that may halt or reverse the structural progression of OA. The replacement of the joint with a prosthesis appears to be the best option in the advanced stages of the disease. A formulation (BIOF2) for cartilage regeneration has been recently developed. The present study evaluated the effects of BIOF2 on gene expression in human cell cultures, followed by efficacy trials in three OA animal models. Human synovial fluid cells that were exposed to the formulation exhibited increased transcription factor SOX-9 (SOX9; chondrogenic factor) expression, and decreased mimecan (mineralization inducer) and macrophage-stimulating protein receptor (osteoclastogenic factor) expression. The intra-articular application of BIOF2 in the animal models significantly increased cartilage thickness from 12 to 31% at 28 days, compared with articular cartilage treated with saline solution. The articular area and number of chondrocytes additionally increased significantly, maintaining an unaltered chondrocyte/mm2 proportion. Evaluation of the histological architecture additionally displayed a decrease in the grade of articular damage in the groups treated with BIOF2. In conclusion, BIOF2 has proven to be effective for treating OA in animal models, most likely due to SOX9 overexpression in articular cells.

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Section: Methodsmentioning

confidence: 99%

A promising novel formulation for articular cartilage regeneration: Preclinical evaluation of a treatment that produces SOX9 overexpression in human synovial fluid cells

Delgado‐Enciso

García²,

Hernández

et al. 2017

Mol Med Report

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The Effect of Cost Effective and Useful Diets on Blood Parameters in Female Mice

Minaei¹,

Fazelipour²,

Tootian³

et al. 2017

FNS

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The high rate of cardiovascular disease is due to excessive intake of animal protein. The aim of this study was to investigate the effects of the replacement of the soybean as a cost effective protein on the blood parameters. 110 immature female Balb/c mice three weeks of age were randomized, into eleven groups of 10 animals each based on following diets: 1) low protein, 2) full protein without soybean, 3) full protein with 20% soybean, and 4) full protein with 40% soybean,. The animals received their diet orally on a daily basis for three and six months. At the end of the study period, the blood samples were collected and blood parameters were measured. The data were analyzed with SPSS software using one-way ANOVA and Tukey's test. Results: In the groups that received 20% and 40% soybean diet, the cholesterol, LDH, glucose, creatinine and urea levels showed meaningful decrease, and total protein level showed a significant increase in comparison with the other groups (P < 0.01). However, the mean values of ALT, AST, ALP, triglyceride, calcium and phosphorus did not show significant change among experimental groups (P < 0.01). Results of the present study indicated that soybean as a cost effective protein could be suitable replacement for animal protein and soybean may have beneficial effects on health like reduction of cholesterol, LDH and urea.

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Histomorphometric alteration of knee articular cartilage and serum alkaline phosphatase in young female mice by chronic supplementation with soybean

Cited by 2 publications

References 20 publications

A promising novel formulation for articular cartilage regeneration: Preclinical evaluation of a treatment that produces SOX9 overexpression in human synovial fluid cells

A promising novel formulation for articular cartilage regeneration: Preclinical evaluation of a treatment that produces SOX9 overexpression in human synovial fluid cells

The Effect of Cost Effective and Useful Diets on Blood Parameters in Female Mice

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