Histomorphological, VEGF and TGF-β immunoexpression changes in the diabetic rats’ ovary and the potential amelioration following treatment with metformin and insulin

Ali, Eyad M.T.; Abdallah, Hesham I.; El-Sayed, Salwa M.

doi:10.1007/s10735-020-09880-x

Cited by 6 publications

(4 citation statements)

References 92 publications

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“…Future studies in the chicken should investigate the mechanisms in which metformin is able to improve the follicular hierarchy. Consistent with our findings, previous studies have reported a 'normalization' of the ovarian follicular profile in both rats (Di Pietro et al 2015, Rencbar et al 2018, Ali et al 2020) and humans (Fleming et al 2002, Morley et al 2017 and has been reported to increase ovulation rates (Nestler et al 1998, 2002, Fleming et al 2002 as well as a return to normal menstrual cyclicity (Velazquez et al 1994, Morin-Papunen et al 1998, Moghetti et al 2000, van Santbrink et al 2005 in women with PCOS.…”

Section: Discussionsupporting

confidence: 93%

Metformin Improves Ovarian Function and Increases Egg Production in Broiler Breeder Hens

Weaver

Ramachandran

2022

Reproduction

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Broiler breeder hens, the parent stock of commercial broiler chickens, have poor reproductive efficiency associated with aberrant and excessive recruitment of ovarian follicles which results in sub-optimal egg production, fertility, and hatchability. The reproductive dysfunction observed in these hens resembles polycystic ovary syndrome in women, a condition wherein metformin is prescribed as a treatment. The main objectives of this study were to determine the effect of metformin on body weight, abdominal fat pad weight, ovarian function, and plasma steroid hormone concentrations. Broiler breeder hens were treated with 0, 25, 50, or 75 mg/kg body weight of metformin mixed in the diet for 40 weeks (n= 45 hens/treatment; 25-65 weeks of age). At 65 weeks of age, hens that received the highest dose of metformin had significantly lower body and abdominal fat pad weights (p < 0.05) than the control. Metformin treatment, at all levels, normalized the preovulatory and prehierarchical ovarian follicular hierarchy. Metformin (50 or 75 mg/kg body weight) significantly increased the total number of eggs laid per hen during the entire production period and these hens had significantly greater fertility and hatchability at 65 weeks of age compared to the control (p < 0.05). Metformin treatment at all levels altered the plasma profile of reproductive hormones, with significantly lower plasma testosterone concentrations and a decreased testosterone to androstenedione ratio in hens that received metformin (p < 0.05). Future studies should focus on the mechanisms underlying the beneficial effects of metformin in improving the reproductive efficiency of broiler breeder hens.

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Section: Discussionsupporting

confidence: 93%

Metformin Improves Ovarian Function and Increases Egg Production in Broiler Breeder Hens

Weaver

Ramachandran

2022

Reproduction

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“…Our findings about the decline in the number of all types of follicles in the HFD group were consistent with the previous results of research in diabetics [ 36 , 37 ].…”

Section: Discussionsupporting

confidence: 93%

Effect of apple vinegar on folliculogenesis and ovarian kisspeptin in a high-fat diet-induced nonalcoholic fatty liver disease in rat

et al. 2022

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Background Non-alcoholic fatty liver disease (NAFLD) adversely affects reproduction. We aimed to study the effect of a high-fat diet (HFD), supplemented with apple vinegar, on folliculogenesis in a rat model of NAFLD. Methods Female rats were randomly divided into four groups (N = 28): Standard diet (SD), SD + vinegar, HFD, and HFD + vinegar groups. At the end of the study, biochemical tests were assessed in serum. HOMA-IR (Homeostatic model assessment-Insulin resistance) was calculated. Sex hormones were determined using an ELISA kit; ovary follicle counts were studied using histological methods. The proliferation index of granulosa cells was determined using immunohistochemistry. Kisspeptin expression in the ovary was detected using RT-PCR. Results The HFD induced steatohepatitis and NAFLD. The ovaries in the rat model of NAFLD were atrophied. The ovaries had less count of developing follicles and corpus luteum, and more degenerated and cystic follicles in comparison with the SD group. Vinegar + HFD consumption decreased ALT, compared to the HFD group (P = 0.004). Steatohepatitis was reduced in the Vinegar + HFD group (P = 0.001). Vinegar + HFD considerably reduced HOMA-IR (p = 0.01). The HFD + vinegar diet could increase estradiol (P = 0.001), without significantly affecting progesterone or testosterone. In addition, an increase of primordial follicles as an ovarian reserve and also primary follicles were determined in the HFD + vinegar group. There were no statistical differences in the granulosa cell proliferation index in various follicle types between groups. HFD + vinegar significantly enhanced ovarian kisspeptin expression (p = 0.04). Conclusions The vinegar diet in a rat model of NAFLD raises estradiol, primordial, and small primary follicles, and increases ovarian kisspeptin expression indirectly. Insulin resistance and obesity were improved by apple vinegar, and anti-glycemic and anti-lipidemic effects were also determined. The supplementation of apple vinegar in NAFLD might be useful for ovary. However, it requires further investigation.

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“…The ovarian dysfunction encountered in broiler breeder hens has previously been associated with excessive follicular recruitment, leading to a double preovulatory follicular hierarchy and increased incidence of double ovulation, which are often non-viable. Consistent with our findings, previous studies have reported a 'normalization' of the ovarian follicular profile in both rats (Ali et al, 2020;Di Pietro et al, 2015;Rencbar et al, 2018) and humans (Fleming et al, 2002;Morley et al, 2017) and has been reported to increase ovulation rates (Fleming et al, 2002;Nestler et al, 2002Nestler et al, , 1998 as well as a return to normal menstrual cyclicity (Moghetti et al, 2000;Morin-Papunen et al, 1998;van Santbrink et al, 2005;Velazquez et al, 1994) in women with PCOS.…”

Section: Discussionsupporting

confidence: 92%

“…Consistent with our findings, previous studies have reported a ‘normalization’ of the ovarian follicular profile in both rats (Di Pietro et al, 2015; Rencbar et al, 2018; Ali et al, 2020) and humans (Fleming et al, 2002; Morley et al, 2017) and has been reported to increase ovulation rates (Nestler et al, 1998, 2002; Fleming et al, 2002) as well as a return to normal menstrual cyclicity (Velazquez et al, 1994; Morin-Papunen et al, 1998; Moghetti et al, 2000; van Santbrink et al, 2005) in women with PCOS.…”

Section: Discussionsupporting

confidence: 92%

Metformin Improves Ovarian Function and Increases Egg Production in Broiler Breeder Hens

Weaver

Ramachandran

2022

Preprint

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Broiler breeder hens, the parent stock of commercial broiler chickens, have poor reproductive efficiency associated with aberrant and excessive recruitment of ovarian follicles which results in sub-optimal egg production, fertility, and hatchability. The reproductive dysfunction observed in these hens resembles polycystic ovary syndrome in women, a condition wherein metformin is prescribed as a treatment. The main objectives of this study were to determine the effect of metformin on body weight, abdominal fat pad weight, ovarian function, and plasma steroid hormone concentrations. Broiler breeder hens were treated with 0, 25, 50, or 75 mg/kg body weight of metformin mixed in the diet for 40 weeks (n= 45 hens/treatment; 25-65 weeks of age). At 65 weeks of age, hens that received the highest dose of metformin had significantly lesser body and abdominal fat pad weights (p < 0.05) than the control. Metformin treatment, at all levels, normalized the preovulatory and prehierarchical ovarian follicular hierarchy and significantly increased the total number of eggs laid per hen during the entire production period in hens that received 50 or 75 mg/kg body weight compared to the control. Fertility and hatchability of eggs were not affected by metformin treatment. Metformin treatment at all levels altered the plasma profile of reproductive hormones, with significantly lower plasma testosterone concentrations and a decreased testosterone to androstenedione ratio in hens that received metformin (p < 0.05). Future studies should focus on the mechanisms underlying the beneficial effects of metformin in improving the reproductive efficiency of broiler breeder hens.

show abstract

Histomorphological, VEGF and TGF-β immunoexpression changes in the diabetic rats’ ovary and the potential amelioration following treatment with metformin and insulin

Cited by 6 publications

References 92 publications

Metformin Improves Ovarian Function and Increases Egg Production in Broiler Breeder Hens

Metformin Improves Ovarian Function and Increases Egg Production in Broiler Breeder Hens

Effect of apple vinegar on folliculogenesis and ovarian kisspeptin in a high-fat diet-induced nonalcoholic fatty liver disease in rat

Metformin Improves Ovarian Function and Increases Egg Production in Broiler Breeder Hens

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