Histomorphological testicular changes and decrease in the sperm count in pubertal rats induced by a high-sugar diet

León-Ramírez, Yeimy Mar De; Lara-García, Miguel; Pacheco, Pablo; Lara-García, Omar; Martı́nez–Gómez, Margarita; Cuevas-Romero, Estela; Rodríguez-Antolín, Jorge; Nicolás-Toledo, Leticia

doi:10.1016/j.aanat.2021.151678

Cited by 5 publications

(12 citation statements)

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“…After decapitation, animals were placed in a supine position and made a longitudinal incision on the abdominal cavity. Both testes with the epididymis and PAT were harvested and weighed (g tissue/100 g BW) (De León-Ramírez et al, 2021). eosin (H&E) (De León-Ramírez et al, 2021).…”

Section: Obtaining Tissuesmentioning

confidence: 99%

“…Both testes with the epididymis and PAT were harvested and weighed (g tissue/100 g BW) (De León-Ramírez et al, 2021). eosin (H&E) (De León-Ramírez et al, 2021). Sections were visualized and photographed using a light microscope (Zeiss Axio Imager A1) at ×2.5, ×4, ×10, and ×40.…”

Section: Obtaining Tissuesmentioning

confidence: 99%

“…Although the daily recommendation is 5%-10% of carbohydrates from the total energy intake in adults and children (Organization for Economic Co-operation and Development (OECD), 2017), the worldwide consumption of sugars has increased from 169 to almost 180 million metric tons (Murphy and Johnson, 2003; United States Department of Agriculture (USDA), 2020). This increase in the sugar consumption is present from gestation (Gamba et al, 2019;Casas et al, 2020) and breastfeeding (Zou et al, 2012), continuing throughout childhood (Dubois et al, 2007;De León-Ramírez et al, 2021) and adult life (Kumar et al, 2014). During gestation and breastfeeding, nutrition through the maternal diet plays a critical role (Cervantes-Rodríguez et al, 2014;Nicolás-Toledo et al, 2018;Pedrana et al, 2020), it is already well known that imbalances in the adequate consumption of macronutrients, such as proteins (Pedrana et al, 2020) and carbohydrates (Casas et al, 2020), negatively affect the development and maturation of different organs in later stages (Cervantes-Rodríguez et al, 2014;Nicolás-Toledo et al, 2018;Corona-Quintanilla et al, Online ahead of print) because both the glucose and fructose cross the placenta (Holmberg et al, 1956) and fetal development depends on transport of glucose through the mother's blood (Regnault et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Both high-calorie diets (Jastrzębska et al, 2014;De León-Ramírez et al, 2021) and a BMI > 25 kg/m 2 (overweight and obesity) (Organization for Economic Co-operation and Development (OECD), 2017; Morales et al, 2014) is associated with male reproductive tract diseases related to some metabolic disorders (metabolic syndrome, insulin resistance, and dyslipidemias) (Nicolás-Toledo et al, 2018;De León-Ramírez et al, 2021). In animal models, high-carbohydrate diets have been established depending on the type and amount of mono, di, or polysaccharide used (Rodríguez-Correa et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Also, it has already been described that consumption of these diets during pregnancy and postnatal stages can affect the function of the striated muscles associated with copulation (Corona-Quintanilla et al, Online ahead of print) and the expression of factors related to fat, such as the insulin receptor and the development of spermatogonias (Mao et al, 2018). In addition, postnatal consumption negatively affects testicular histology, injuring the intra and extra tubular epithelium (De León-Ramírez et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Maternal and Offspring Sugar Consumption Increases Perigonadal Adipose Tissue Hypertrophy and Negatively Affects the Testis Histological Organization in Adult Rats

Gabriela

Nicolás-Toledo²,

Cervantes-Rodríguez³

et al. 2022

Front. Cell Dev. Biol.

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Sugar intake has been associated with the development of male reproductive pathologies because of the increase and dysfunction in different adipose tissue depots. The establishment of these dysfunctions in the early stages of development is unknown. We evaluated the effect of maternal (pregnancy and lactation) and male offspring (from weaning to adulthood) consumption of 5% sucrose on perigonadal adipose tissue (PAT) and testis in adulthood. Moreover, two rat groups were compared, both including pregnant and lactating females: Control (C—drinking tap water) and sugar (S—consuming 5% sucrose solution). From weaning to adulthood with male offspring, four subgroups were formed: Control Mother → Control and Sugar offspring (CC, CS) and Sugar Mother → Control and Sugar offspring (SC, SS). At 120 postnatal days, the testes and PAT were collected and morphologically described. Furthermore, we quantified the number and cross-sectional area of perigonadal adipocytes and their distribution. We found that the males from SC and SS groups showed high PAT weight (p < 0.005), a high number (p < 0.05), and a relative frequency of large adipocytes (p < 0.05), establishing these results during gestational and lactation stages, and enhancing in adulthood since postnatal diet and its interaction. More macrophages, mast cells, and Leydig cells were observed in the interstitial space of the testis for the CS, SC, and SS groups, concluding that consumption of a high-carbohydrate maternal diet, program hypertrophy processes in adult PAT, developing and enhancing with sugar consumption during postnatal life. Furthermore, they are associated with inflammatory processes within the interstitial space of the testis.

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Section: Obtaining Tissuesmentioning

confidence: 99%

Section: Obtaining Tissuesmentioning

confidence: 99%